Precise spatial localization of broadleaf species is crucial for efficient forest management and ecological studies. This study presents an advanced approach for segmenting and classifying broadleaf tree species, including Japanese oak (Quercus crispula), in mixed forests using multi-spectral imagery captured by unmanned aerial vehicles (UAVs) and deep learning. High-resolution UAV images, including RGB and NIR bands, were collected from two study sites in Hokkaido, Japan: Sub-compartment 97g in the eastern region and Sub-compartment 68E in the central region. A Mask Region-based Convolutional Neural Network (Mask R-CNN) framework was employed to recognize and classify single tree crowns based on annotated training data. The workflow incorporated UAV-derived imagery and crown annotations, supporting reliable model development and evaluation. Results showed that combining multi-spectral bands (RGB and NIR) with canopy height model (CHM) data significantly improved classification performance at both study sites. In Sub-compartment 97g, the RGB + NIR + CHM achieved a precision of 0.76, recall of 0.74, and F1-score of 0.75, compared to 0.73, 0.74, and 0.73 using RGB alone; 0.68, 0.70, and 0.66 with RGB + NIR; and 0.63, 0.67, and 0.63 with RGB + CHM. Similarly, at Sub-compartment 68E, the RGB + NIR + CHM attained a precision of 0.81, recall of 0.78, and F1-score of 0.80, outperforming RGB alone (0.79, 0.79, 0.78), RGB + NIR (0.75, 0.74, 0.72), and RGB + CHM (0.76, 0.75, 0.74). These consistent improvements across diverse forest conditions highlight the effectiveness of integrating spectral (RGB and NIR) and structural (CHM) data. These findings underscore the value of integrating UAV multi-spectral imagery with deep learning techniques for reliable, large-scale identification of tree species and forest monitoring.

The growing focus on the role of forests in carbon sequestration highlights the importance of accurately and efficiently measuring biophysical traits, such as diameter at breast height (DBH) and tree height. Understanding genetic contributions to trait variation is crucial for enhancing carbon storage through the genetic improvement of forest trees. Light detection and ranging (LiDAR) has been used to estimate DBH and tree height; however, few studies have explored the heritability of these traits or assessed the accuracy of biomass increment selection based on them. Therefore, this study aimed to leverage LiDAR to measure DBH and tree height, estimate tree heritability, and evaluate the accuracy of timber volume selection based on these traits, using 60-year-old larch as the study material. Unmanned aerial vehicle laser scanning (ULS) and backpack laser scanning (BLS) were compared against hand-measured values. The accuracy of DBH estimations using BLS resulted in a root mean square error (RMSE) of 2.7 cm and a coefficient of determination of 0.67. Conversely, the accuracy achieved with ULS was 4.0 cm in RMSE and a 0.24 coefficient of determination. The heritability of DBH was higher with BLS than with ULS and even exceeded that of hand measurements. Comparisons of timber volume selection accuracy based on the measured traits demonstrated comparable performance between BLS and ULS. These findings underscore the potential of using LiDAR remote sensing to quantitatively measure forest tree biomass and facilitate their genetic improvement of carbon-sequestration ability based on these measurements.