Populus deltoides is one of the primary tree species for bioenergy production in temperate regions. In arid/semi-arid northern China, the scarcity of water and nitrogen significantly limits the productivity of poplar plantations. The identification of relevant molecular markers can promote the breeding of resource-efficient varieties. In this study, 188 genotypes of P. deltoides from six provenances served as experimental material. Genetic differentiation analysis, analysis of molecular variance (AMOVA), principal coordinate analysis (PCoA), unweighted pair group method with arithmetic mean (UPGMA) clustering, and genetic structure analysis were performed using selected simple sequence repeat (SSR) markers. Based on these analyses, the association analysis of water-use efficiency (WUE) and nitrogen-use efficiency (NUE) were conducted using general linear model (GLM) and mixed linear model (MLM) approaches. The results showed that 15 pairs of SSR primers successfully amplified across all 188 individuals, with an average of 7.33 alleles (Na) observed per primer pair. The polymorphism information content (PIC) ranged from 0.060 to 0.897, with an average of 0.544, indicating high genetic diversity in the selected markers. The average inbreeding coefficient intra-population (Fis), inbreeding coefficient inter-population (Fit), and inter-population genetic fraction coefficient (Fst) values were 0.005, 0.135, and 0.132, respectively, indicating high heterozygosity, substantial inbreeding within populations, and moderate genetic differentiation, with an average gene flow (Nm) of 1.964, suggesting substantial gene flow between populations. Additionally, molecular variance was primarily within individuals (84.12%). Genetic structure analysis revealed four subgroups, with some degree of genetic admixture among the provenances. In the GLM model, 11 markers were significantly associated with five traits (p < 0.05), with an average contribution rate of 15.82%. Notably, SSR132 and SSR143 were significantly associated with multiple traits (p < 0.05). The MLM model identified two markers (SSR47 and SSR85) significantly associated with ground diameter (p < 0.05) and one marker (SSR80) significantly associated with NUE (p < 0.05). This study identifies loci associated with WUE and NUE, laying a foundation for future genetic improvement and marker-assisted breeding strategies in poplar.

This study aims to evaluate the growth characteristics of six Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) provenances (S1–S6) from different climatic regions in subtropical China in order to select superior provenances with strong adaptability, fast growth, and reasonable biomass allocation. These results will provide references for genetic improvement and resource utilization of Chinese fir plantations. A total of 385 trees, aged 26 to 48 years, were selected from the Chinese fir gene bank in Anhui. Wood core sampling was used to obtain tree ring width and early/latewood width data. Growth rate, fast-growth period, and biomass allocation of each provenance were analyzed using methods such as the logistic growth equation, BAI (basal area increment), latewood percentage, and biomass estimation. The fast-growth period of Chinese fir starts from the 2nd to the 4th year, with significant growth occurring around the 14th year and growth stabilizing between 30 and 50 years. Provenance S2 showed clear advantages in growth rate and biomass, while S6 was relatively weak. BAI analysis revealed that the provenances reached their growth peak around 10 years of age, with a gradual decline afterward, but S2 maintained higher growth levels for a longer period. Root-shoot ratio analysis showed that S2 had the most balanced ratio, promoting stable growth and efficient water and nutrient absorption, while S6 had a higher root-shoot ratio, indicating growth limitations. Furthermore, S2 demonstrated continuous biomass increase after 30 years, indicating excellent growth potential. This study provides quantitative analysis of the growth characteristics and adaptability of different Chinese fir provenances, offering scientific support for the construction and breeding of Chinese fir plantations, and contributing to enhancing the productivity and ecological adaptability of Chinese fir plantations for sustainable resource utilization.

In the subtropical mountainous area of Huangshan, China, 81 Torreya grandis were selected for research on growth and element distribution, with the aim of providing empirical and theoretical support for the promotion and management of T. grandis. Seven growth equations were constructed for tree height (H) and diameter at breast height (DBH) in age levels 40–2000. The most suitable Korf equation was selected by comparing the R-squared values. With R-squared values of 0.93 and 0.80, the equations H = 33.98 × Exp(−53.56/age^0.84) and DBH = 158.48 × Exp(−21.41/age^0.55) fit T. grandis growth best, respectively. The results show that T. grandis growth was relatively rapid before the age of 45 and then slowed. Among the carbon (C), nitrogen (N), and phosphorus (P) elements found in different T. grandis organs of the same age, the roots had the lowest C content, the leaves had the highest N content, and the branches had the lowest P content. The roots had the highest C:N and C:P, and the leaves had the highest N:P. The amount of N in the leaves and P in the branches both increased significantly with age, and the N:P ratio indicates that considering only one organ may not accurately indicate T. grandis nutrient limitations.

Poplars are crucial for timber supply and ecological protection in China. Enhancing the growth of poplar plantations and improving soil fertility in arid, and semi-arid poor soil regions are key aspects of sustainable forest management. Fertilization (FTL) and drip irrigation (DI) are among the most widely used methods globally for increasing yield and soil productivity. This study conducted field experiments on FTL and DI in a 10-year-old Populus × canadensis ‘Zhongliao 1’ (cultivation varieties of P. canadensis in northern China) plantation. DI limits were set according to soil moisture at 60% (S1), 70% (S2), and 80% (S3) of field capacity; nitrogen FTL rates were set at 100% of the baseline fertilization amount (100% BFA, N 643.20 g·year⁻¹, P 473.37 g·year⁻¹, and K 492.29 g·year⁻¹) (F1), 70% BFA (F2), 130% BFA (F3), and 160% BFA (F4). The treatments of drip irrigation and fertigation (DIF) were H1 (100% BFA, 60% FC), H2 (100% BFA, 80% FC), H3 (160% BFA, 60% FC), and H4 (160% BFA, 80% FC), along with a control group (CK) without any management, totaling 12 experimental combinations. The results showed that the H4 had the most significant promoting effect on the height, DBH, and volume increments. All treatments had little effect on the soil bulk density of the plantation but significantly impacted soil capillary porosity and pH. Compared to DI, soil nutrient and organic matter content were more sensitive to FTL. Appropriate FTL and DI can increase soil sucrase activity. Soil urease activity tended to increase with higher FTL rates, and higher DI levels also positively influenced urease activity. Excessive or insufficient soil moisture and nutrients negatively impacted soil cellulase and catalase activities. Correlation analysis revealed no significant correlation between the growth of P. × canadensis ‘Zhongliao 1’ and soil nutrient content, but significant or highly significant correlations existed between growth and soil porosity and related enzyme activities. Comprehensive evaluation using a membership function indicated that high FTL levels (F4) were more conducive to the simultaneous improvement of the growth and soil fertility of the plantation, followed by H4 and F1, suggesting that high FTL is the key factor affecting the growth of 10-year-old P. × canadensis ‘Zhongliao 1’ plantations and the restoration of stand productivity, with moisture being secondary.

Poplar is an important tree species for timber supply and ecological protection in northern China. Cultivating and selecting high-quality varieties and germplasm resources suitable for cultivation are key factors in enhancing the quality and productivity of poplar plantations in the arid and semi-arid northern regions with shorter growing seasons. This study conducted a field cultivation experiment on 10 progeny clones from the direct cross (D × M) of imported Populus deltoides ‘DD-109’ with Populus maximowiczii and 7 progeny clones from the reciprocal cross (M × D) using one-year-old rooted cuttings planted at a 4 m × 8 m spacing. Based on 17 years of annual growth observations, the study systematically compared growth characteristics, age of quantitative maturity, path relationships between traits, and early selection efficiency in the hybrid offspring. The results indicated that the D × M population had superior diameter at breast height (DBH), tree height (H), and volume (V) compared to the M × D population, while the height-to-diameter ratio (HDR) was lower. The growth rate of the 17 clones peaked from 10 to 14 years, with annual volume growth increments (PAIs) higher than mean annual volume increments (MAIs) during the early growth stages; the quantitative maturity age ranged between 12 and 16 years. The D × M population generally reached quantitative maturity earlier than the M × D population, with the fastest clone maturing in 12 years. Four clones (DM-9-17, DM-9-18, DM-9-14, and MD-61) showed values for V, DBH, H, and HDR above the hybrid group average. Path analysis demonstrated that DBH had the most significant direct and indirect effects on V, suggesting it as the best predictor for V. Using DBH as a reference, correlation and early selection efficiency analysis showed a strong relationship between growth characteristics at planting years 4–5 and later-stage performance, indicating this as the optimal period for early selection. These findings contribute to evaluating the production potential of P. deltoides ‘DD-109’ and P. maximowiczii germplasm in northern China and provide valuable guidance for selecting poplar clones suitable for local cultivation, accelerating breeding processes, and informing management planning for poplar plantations.