LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES2-LIKEs (LBDs/ASLs) are plant-specific transcription factors that function downstream of auxin-regulated lateral root (LR) formation. Our previous research found that PpLBD16 positively regulates peach (Prunus persica) LR formation. However, the downstream regulatory network and target genes of PpLBD16 are still largely unknown. Here, we constructed a PpLBD16 homologous overexpression line and a PpLBD16 silenced line. We found that overexpressing PpLBD16 promoted peach root initiation, while silencing PpLBD16 inhibited peach root formation. Through RNA sequencing (RNA-seq) analysis of roots from PpLBD16 overexpression and silenced lines, we discovered that genes positively regulated by PpLBD16 were closely related to cell wall synthesis and degradation, ion/substance transport, and ion binding and homeostasis. To further detect the binding motifs and potential target genes of PpLBD16, we performed DNA-affinity purification sequencing (DAP-seq) analysis in vitro. PpLBD16 preferentially bound to CCNGAAANNNNGG (MEME-1), [C/T]TTCT[C/T][T/C] (MEME-2), and GCGGCGG (ABR1) motifs. By combined analysis of RNA-seq and DAP-seq data, we screened candidate target genes for PpLBD16. We demonstrated that PpLBD16 bound and activated the cell wall modification-related genes EXPANSIN-B2 (PpEXPB2) and SUBTILISIN-LIKE PROTEASE 1.7 (PpSBT1.7), the ion transport-related gene CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1 (PpCNGC1) and the polyphenol oxidase (PPO)-encoding gene PpPPO, thereby controlling peach root organogenesis and promoting LR formation. Moreover, our results displayed that PpLBD16 and its target genes are involved in peach LR primordia development. Overall, this work reveals the downstream regulatory network and target genes of PpLBD16, providing insights into the molecular network of LBD16-mediated LR development.

Microbial fertilizers have a significant role in promoting plant growth, resistance to environmental stresses, and soil remediation. Microbial fertilizers are mainly composed of beneficial microorganisms that contain specific functions. Focusing on the peach tree rhizosphere region, this study aimed to isolate and screen bacteria with efficient phosphate-solubilizing capacity for application in microbial fertilizers, as well as to dig deeper into the other properties of the strains to further explore the roles of these phosphate-solubilizing bacteria (PSBs) in terms of plant growth in order to provide valuable microbial resources for microbial fertilizer development. By collecting soil samples from peach tree rhizospheres, we initially screened 86 PSB strains using the plate method and determined the phosphate-solubilizing capacity (ranged from 0 to 14 μg/mL). Afterwards, 51 strains with strong phosphate-solubilizing capacity were selected for molecular identification; the strains belonged to 12 genera, with Bacillus and Burkholderia accounting for the majority. Concurrent evaluation of iron carriers and indoleacetic-3-acid (IAA) production capabilities identified strain WPD85 as exhibiting dual functionality with strong performance in both parameters. Subsequently, we combined the analysis of phosphate-solubilizing capacity and growth-promoting properties to select eight strains of PSBs; characterized them physiologically, biochemically, and molecularly; determined the biofilm-forming capacity; and conducted potting experiments. Notably, strain WPD103 exhibited exceptional biofilm-forming capacity (OD₅₉₅ = 1.09). Of particular interest, strain WPD16 demonstrated both an elevated inorganic phosphate solubilization index (D/d = 2.99) and remarkable iron carriers production capacity, while peach seedlings treated with WPD16 exhibited 119% enhancement in plant height increment compared to the control. This study enhances our understanding of PSB traits and identifies Burkholderia sp. WPD16 as a strategic candidate for developing targeted microbial fertilizers, offering a sustainable solution to reduce reliance on chemical inputs in orchard management.

Peach (Prunus persica (L.) Batsch) is a fruit tree of economic and nutritional importance, but it is very sensitive to drought stress, which affects its growth to a great extent. Lauric acid (LA) is a fatty acid produced in plants and associated with the response to abiotic stress, but the underlying mechanism remains unclear. In this study, physiological analysis showed that 50 ppm LA pretreatment under drought stress could alleviate the growth of peach seedlings. LA inhibits the degradation of photosynthetic pigments and the closing of pores under drought stress, increasing the photosynthetic rate. LA also reduces the content of O₂⁻, H₂O₂, and MDA under drought stress; our results were confirmed by Evans Blue, nitroblue tetrazolium (NBT), and DAB(3,3-diaminobenzidine) staining experiments. It may be that, by directly removing reactive oxygen species (ROS) and improving enzyme activity, i.e., catalase (CAT) activity, peroxidase (POD) activity, superoxide dismutase (SOD) activity, and ascorbate peroxidase (APX) activity, the damage caused by reactive oxygen species to peach seedlings is reduced. Peach seedlings treated with LA showed a significant increase in osmoregulatory substances compared with those subjected to drought stress, thereby regulating osmoregulatory balance and reducing damage. RNA-Seq analysis identified 1876 DEGs (differentially expressed genes) in untreated and LA-pretreated plants under drought stress. In-depth analysis of these DEGs showed that, under drought stress, LA regulates the expression of genes related to plant–pathogen interaction, phenylpropanoid biosynthesis, the MAPK signaling pathway, cyanoamino acid metabolism, and sesquiterpenoid and triterpenoid biosynthesis. In addition, LA may activate the Ca²⁺ signaling pathway by increasing the expressions of CNGC, CAM/CML, and CPDK family genes, thereby improving the drought resistance of peaches. In summary, via physiological and transcriptome analyses, the mechanism of action of LA in drought resistance has been revealed. Our research results provide new insights into the molecular regulatory mechanism of the LA-mediated drought resistance of peach trees.

Summary: Root development is crucial for the growth and yield of horticultural crops. Hydrogen sulfide (H₂S), an important gasotransmitter, has been shown to regulate lateral root (LR) formation in plants, including peach (Prunus persica). However, its specific regulatory mechanism remains largely unclear. Here, we show that the energy/metabolic sensor SUCROSE NON‐FERMENTING RELATED KINASE 1 (SnRK1) mediates the control of peach LR growth by H₂S. PpSnRK1 activity in peach roots is enhanced with H₂S to promote LR generation. Cys419, Cys430 and Cys505 residues in the catalytic α‐subunit of PpSnRK1 are modified by H₂S persulfidation. Reduced persulfidation inhibits the H₂S‐induced PpSnRK1 activity. Mutating Cys419 and Cys430 of PpSnRK1α severely impedes H₂S‐promoted LR formation. LATERAL ORGAN BOUNDARIES DOMAIN 16 (LBD16) is a transcription factor essential for LR initiation. Further evidence shows that PpSnRK1α interacts with PpLBD16 in the nucleus, thereby enhancing the transcription of LR development‐related genes PpCNGC1 (CYCLIC NUCLEOTIDE‐GATED ION CHANNEL 1) and PpEXPB2 (EXPANSIN‐B2). In peach roots, transcription of these two genes is markedly up‐regulated by H₂S‐induced PpSnRK1 activity. Silencing of PpLBD16, PpEXPB2 or PpCNGC1 significantly reduces exogenous H₂S‐induced LR formation. In situ hybridization analysis shows that they are strongly expressed in peach LR primordia along with PpSnRK1α. Our data reveal an interaction between H₂S signal and SnRK1 kinase, providing mechanistic insights into the shaping of agronomically important root systems.

Background/Objectives: The sucrose non-fermentation-related kinase 1 (SnRK1) protein complex in plants plays an important role in energy metabolism, anabolism, growth, and stress resistance. SnRK1 is a heterotrimeric complex. The SnRK1 complex is mainly composed of α, β, βγ, and γ subunits. Studies on plant SnRK1 have primarily focused on the functional α subunit, with the β regulatory subunit remaining relatively unexplored. The present study aimed to elucidate the evolutionary relationship, structural prediction, and interaction with the core α subunit of peach SnRK1β3 (PpSnRK1) subunit. Methods: Bioinformatics analysis of PpSnRK1 was performed through software and website. We produced transgenic tomato plants overexpressing PpSnRK1 (OEPpSnRK1). Transcriptome analysis was performed on OEPpSnRK1 tomatoes. We mainly tested the growth index and drought resistance of transgenic tomato plants. Results: The results showed that PpSnRK1 has a 354 bp encoded protein sequence (cds), which is mainly located in the nucleus and cell membrane. Phylogenetic tree analysis showed that PpSnRK1β3 has similar domains to other woody plants. Transcriptome analysis of OEPpSnRK1β3 showed that PpSnRK1β3 is widely involved in biosynthetic and metabolic processes. Functional analyses of these transgenic plants revealed prolonged growth periods, enhanced growth potential, improved photosynthetic activity, and superior drought stress tolerance. Conclusions: The study findings provide insight into the function of the PpSnRK1 subunit and its potential role in regulating plant growth and drought responses. This comprehensive analysis of PpSnRK1 will contribute to further enhancing our understanding of the plant SnRK1 protein complex.