Arbuscular mycorrhizal fungi (AMF) have been shown to play a major role in regulating the accumulation, transport, and toxicity of cadmium (Cd) in plant tissues. This review aims to highlight the current understanding of the mechanisms by which AMF alleviate Cd toxicity in plants. Cd accumulation in agricultural soils has become an increasing global concern due to industrial activities and the use of phosphatic fertilizers. Cd toxicity disrupts various physiological processes in plants, adversely affecting growth, photosynthesis, oxidative stress responses, and secondary metabolism. AMF alleviate Cd stress in plants through multiple mechanisms, including reduced Cd transport into plant roots, improved plant nutritional status, modulation of organic acid and protein exudation, enhanced antioxidant capacity, and maintenance of ion homeostasis. AMF colonization also influences Cd speciation, bioavailability, and compartmentalization within plant tissues. The expression of metal transporter genes, as well as the synthesis of phytochelatins and metallothioneins, are modulated by AMF during Cd stress. However, the efficacy of AMF in mitigating Cd toxicity depends on several factors, such as soil properties, plant species, AMF taxa, and experimental duration. Further knowledge of the intricate plant–AMF–Cd interactions is crucial for optimizing AMF-assisted phytoremediation strategies and developing Cd-tolerant and high-yielding crop varieties for cultivation in contaminated soils.

Salt stress poses a significant challenge to plant growth and restricts agricultural development. To delve into the intricate mechanisms involved in soybean’s response to salt stress and find targets to improve the salt resistance of soybean, this study integrated transcriptomic, proteomic, and metabolomic analyses to explore the regulatory networks involved in soybean salt tolerance. Transcriptomic analysis revealed significant changes in transcription factors, hormone-related groups, and calcium ion signaling. Notably, the biosynthetic pathways of cutin, suberine, and wax biosynthesis play an important role in this process. Proteomic results indicated salt-induced DNA methylation and the enrichment of phosphopyruvate hydrase post-salt stress, as well as its interaction with enzymes from various metabolic pathways. Metabolomic data unveiled the synthesis of various metabolites, including lipids and flavonoids, in soybean following salt stress. Furthermore, the integrated multiomics results highlighted the activation of multiple metabolic pathways in soybean in response to salt stress, with six pathways standing out prominently: stilbenoid, diarylheptanoid, and gingerol biosynthesis; carotenoid biosynthesis; carbon fixation in photosynthetic organisms; alanine, aspartate, and glutamate metabolism; thiamine metabolism; and pyruvate metabolism. These findings not only offer valuable insights into leveraging multiomics profiling techniques for uncovering salt tolerance mechanisms but also identify candidate genes for soybean improvement.

Soil salinization has emerged as a major factor negatively affecting soil quality and plant productivity. Proline, functioning as an osmotic regulator, has been proposed as an effective strategy for enhancing plant tolerance to salt stress. This study aimed to investigate the effects of exogenous proline on salt tolerance in soybeans. A hydroponic experiment was conducted with different salt treatments (without NaCl, -NaCl; with 100 mM NaCl, +NaCl) and with or without 150 mM proline (+Pro, -Pro). The results showed that proline application alleviated salt stress-induced reductions in plant growth, photosynthetic parameters, and chlorophyll content while aiding recovery from leaf chlorosis. Proline treatment improved ion homeostasis by reducing Na⁺ levels and increasing K⁺ and Ca²⁺ contents in the leaves. Salt stress increased malondialdehyde (MDA) and reactive oxygen species (ROS) levels, along with leaf peroxidase (POD) and catalase (CAT) activities, while decreasing superoxide dismutase (SOD) activity. Moreover, salt stress obviously enhanced proline accumulation, accompanied by increases in glutamate (Glu), glutamate-1-semialdehyde (GSA), and pyrroline-5-carboxylate (P5C) content, as well as the activities of pyrroline-5-carboxylate synthase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) in the glutamate pathway, while reducing proline dehydrogenase (ProDH) activity. Exogenous proline treatment further elevated proline content and increased key substances and enzyme activities in both the glutamate (Glu and P5C content, P5CS and P5CR activity) and ornithine (Orn content and OAT activity) pathways while also reducing ProDH activity. Collectively, our results revealed that exogenous proline contributed to an attenuation of salt stress in soybeans by regulating both the glutamate and ornithine pathways to stimulate endogenous proline accumulation, mediate Na⁺/K⁺ homeostasis, and inhibit oxidative damage.

Soil salinization is a major factor threatening global food security. Soil improvement strategies are therefore of great importance in mitigating the adverse effect of salt stress. Our study aimed to evaluate the effect of biochar (BC) and nitric acid-modified biochar (HBC) (1%, 2%, and 3%; m/m) on the properties of salinized soils and the morphological and physiological characteristics of pakchoi. Compared with BC, HBC exhibited a lower pH and released more alkaline elements, reflected in reduced contents of K⁺, Ca²⁺, and Mg²⁺, while its hydrophilicity and polarity increased. Additionally, the microporous structure of HBC was altered, showing a rougher surface, larger pore size, pore volume, specific surface area, and carboxyl and aliphatic carbon content, along with lower aromatic carbon content and crystallinity. Moreover, HBC application abated the pH of saline soil. Both BC and HBC treatments decreased the sodium absorption rate (SAR) of saline soil as their concentration increased. Conversely, both types of biochar enhanced the cation exchange capacity (CEC), organic matter, alkali-hydrolyzable nitrogen, and available phosphorus and potassium content in saline soils, with HBC demonstrating a more potent improvement effect. Furthermore, biochar application promoted the growth-related parameters in pakchoi, and reduced proline and Na⁺ content, whilst increasing leaf K⁺ content under salt stress. Biochar also enhanced the activity of key antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)) in leaves, and reduced hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) content. Collectively, modified biochar can enhance soil quality and promote plant growth in saline soils.