The effectiveness of co-additives for improving livestock waste composting (reduction of air pollution and conservation of nutrients) was investigated. Biochar and Flue gas desulphurization gypsum (FGD gypsum) were used to supplement the composting of a mixture of slaughter waste, swine slurry, and sawdust. Different compositions of additives (0% or 5% each, 10% biochar or FGD gypsum) were tested in triplicate on the laboratory scale. In addition, the effects of two different aeration schemes (continuous and intermittent) were also investigated. Ammonia volatilization, physicochemical characteristics, and compost maturity indices were investigated. The results indicated that the use of the co-additive (Biochar and FGD gypsum) during composting of livestock waste led to a reduction of ammonia volatilization by 26–59% and to a 6.7–7.9-fold increase of nitrate accumulation. The total ammonia volatilization of intermittent aeration treatment was lower than that of continuous aeration using co-additives treatment. It was concluded that co-additives (biochar and FGD gypsum) might be utilized in livestock waste composting to reduce ammonia volatilization and improve nutrient conservation.

Journal of Animal Science and Technology (JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare.

The codigestion of slaughter waste with animal manure can improve its methane yield, and digestion parameters; however, limited studies are available for the effectiveness of anaerobic codigestion using swine slaughter waste (SSW) and swine slurry (SS). Hence, this study was conducted to determine the characteristics of SSW and the effect of anaerobic codigestion with (SS) and explored the potential of CH₄ production (M_max), the lag phase period (λ), and effective digestion time (T_eff). SSW contains fat and protein contents of 54% and 30% dry weight within 18.2% of solid matters, whereas SS showed only 6% and 28% within 4.1% of solid matters, respectively. During sole anaerobic digestion, SSW produced a high M_max (711 Nml CH₄/g VS_added) but had a long duration λ (~9 days); whereas SS produced a low M_max (516 Nml CH₄/g VS_added) but had a shorter duration λ (1 day). Codigestion increased the M_max from 22–84% with no significant T_eff compared to sole SS digestion. However, the low M_max of SS and high M_max of SSW, resulted in a 7–32% decrease in M_max at codigestion compared to SSW sole digestion. Codigestion improved the digestion efficiency as it reduced λ (3.3–8.5 days shorter) and T_eff (6.5–9.1 days faster) compared to SSW sole digestion. The substrate-to-inoculum ratio of 0.5 was better than 1; the volatile solid and micronutrient availability may be attributed to improved digestion. These results can be used for the better management of SSW and SS for bio-energy production on a large scale.

The objective of this study was to determine the potential of LAB with phytate degrading activity from fermented traditional food grain-based and legume-based. Lactic acid bacteria were isolated from different sources of traditional fermented food from Gunungkidul Yogyakarta Indonesia such as gembus tempeh (tofu waste), soybean tempeh, lamtoro tempeh (Leucaena bean) and kara tempeh. Isolation of LAB was performed using Total Plate Count (TPC) on de Man Rogosa Sharpe Agar (MRSA) medium supplemented with CaCO₃. They were screened for their ability to degrade myo-inositol hexaphosphate or IP6 by using qualitative streak platemethod with modified de Man Rogosa-MorpholinoPropanesulfonic Acid Sharpe (MRS-MOPS) medium contained sodium salt of phytic acid as substrate and cobalt chloride staining (plate assay) method. The selected isolates were further assayed for phytase activities using quantitative method with spectrophotometer and the two selected isolates growth were optimized. Furthermore, thhe isolates that shown the highest phytase activity was characterized and identified using API 50 CH kitand 16S rRNA gene sequencing. The results showed that there were 18 LAB isolates obtained from samplesand 13 isolates were able to degrade sodium phytate based on qualitative screening. According to quantitative assay, the highest phytate degrading activities were found in TG-2(23.562 U/mL) and TG-1 (19.641 U/mL) isolated from gembus tempeh. The phytate activity of TG-2 was optimum at 37 °C with agitation, while the phytate activity of TG-1 was optimum at 45 °C without agitation. Characterization and identification of TG-2 isolate with the highest phytate degrading activity using API 50 CH and 16S rRNA showed that TG-2had homology with Lactobacillus fermentum. It could be concluded that LAB from from fermented traditional food grain-based and legume-based produced the extracellular phytase. Keywords: lactic acid bacteria, tempeh, phytatedegrading activity

Objective: Our objective was to examine the relationships of supplemental rumen-protected lysine (RPL) or lysine + methionine (RPLM) on lactational performance, plasma amino acids (AA) concentration, and nitrogen use efficiency of lactating dairy cows by using a meta-analysis approach.Methods: A total of 56 articles comprising 77 experiments with either RPL or RPLM supplementation were selected and analyzed using a mixed model methodology by considering the treatments and other potential covariates as fixed effects and different experiments as random effects.Results: In early lactating cows, milk yield was linearly increased by RPL (β1 = 0.013; p<0.001) and RPLM (β1 = 0.014; p<0.028) but 3.5% fat-corrected milk (FCM) and energy-corrected milk (ECM) (kg/d) was increased by only RPL. RPL and RPLM did not affect dry matter intake (DMI) but positively increased (p<0.05) dairy efficiency (Milk yield/DMI and ECM/DMI). As a percentage, milk fat, protein, and lactose were unchanged by RPL or RPLM but the yield of all components was increased (p<0.05) by feeding RPL while only milk protein was increased by feeding RPLM. Plasma Lys concentration was linearly increased (p<0.05) with increasing supplemental RPL while plasma Met increased (p<0.05) by RPLM supplementation. The increase in plasma Lys had a strong linear relationship (R2 = 0.693 in the RPL dataset and R2 = 0.769 in the RPLM dataset) on milk protein synthesis (g/d) during early lactation. Nitrogen metabolism parameters were not affected by feeding RPL or RPLM, either top-dress or when supplemented to deficient diets. Lactation performance did not differ between AA-deficient or AA-adequate diets in response to RPL or RPLM supplementation.Conclusion: RPL or RPLM showed a positive linear relationship on the lactational performance of dairy cows whereas greater improvement effects were observed during early lactation. Supplementing RPL or RPLM is recommended on deficient-AA diet but not on adequate-AA diet.

Food Science of Animal Resources (Food Sci. Anim. Resour.) is an international, peer-reviewed bimonthly journal publishing original research and reviews on basic and applied aspects of the use of animal resources including the meat, egg, dairy, and edible insect/worm products. Food Sci. Anim. Resour. is published and distributed by the Korean Society for Food Science of Animal Resources.