Development zones are crucial spatial carriers driving economic growth and industrial upgrading, playing a key role in China’s development. After years of expansion, these zones face significant challenges in industrial land development and performance enhancement. This paper takes Suzhou Industrial Park (SIP) as a case, which is a model of Sino–Singaporean government cooperation. Using Landsat 4–5 TM data, socioeconomic data, and industrial land use data, spatial analysis and statistical modeling were employed to examine the evolution and phased patterns of industrial land use in SIP from 1994 to 2022. A performance evaluation system encompassing economic benefits, innovation-driven growth, development intensity, green development, and social security was developed to assess land use performance and its responses to spatial transformations. The results reveal that industrial land in SIP experienced a significant change in the intensity of land expansion from 1.031 to 0.352 during 1994–2022, and the peak circle density expanded from 3 km to 15 km. The mean value of the comprehensive performance score during 2017–2022 was 42.18, with the highest economic efficiency (40.54) and a lower innovation capacity (16.98). The development of industrial land in SIP presents the stage characteristics of monocentric polarization, polycentricity, and spatial diffusion toward a generalized development zone, showing significant path dependence, and the difference in the land use performance of different industrial types is obvious. In the future, the optimization and redevelopment of the stock of land should be strengthened to promote the optimization of the spatial layout of technology-intensive industries and the technological upgrading of labor-intensive industries, as well as achieving sustainable economic growth through innovation-driven, green development and enclave economy collaboration. This study provides a reference for the industrial layout and high-quality sustainable development of development zones.