Ellagic acid, a polyphenolic compound present in fruit and berries, has recently been the object of extensive research for its antioxidant activity, which might be useful for the prevention and treatment of cancer, cardiovascular pathologies, and neurodegenerative disorders. Its protective role justifies numerous attempts to include it in functional food preparations and in dietary supplements, and not only to limit the unpleasant collateral effects of chemotherapy. However, ellagic acid use as a chemopreventive agent has been debated because of its poor bioavailability associated with low solubility, limited permeability, first pass effect, and interindividual variability in gut microbial transformations. To overcome these drawbacks, various strategies for oral administration including solid dispersions, micro and nanoparticles, inclusion complexes, self-emulsifying systems, and polymorphs were proposed. Here, we listed an updated description of pursued micro and nanotechnological approaches focusing on the fabrication processes and the features of the obtained products, as well as on the positive results yielded by in vitro and in vivo studies in comparison to the raw material. The micro and nanosized formulations here described might be exploited for pharmaceutical delivery of this active, as well as for the production of nutritional supplements or for the enrichment of novel foods.

Human neuroblastoma (NB), a pediatric tumor inclined to relapse, after an initial response to therapy, usually develops resistance. Since several chemotherapeutics exert anticancer effect by increasing reactive oxygen species (ROS), NB cells overproduce antioxidant compounds becoming drugs-resistant. A strategy to sensitize NB cells to chemotherapy involves reducing their antioxidant defenses and inducing ROS overproduction. Concerning this, although affected by several issues that limit their clinical application, antioxidant/pro-oxidant polyphenols, such as gallic acid (GA), showed pro-oxidant anti-cancer effects and low toxicity for healthy cells, in several kind of tumors, not including NB. Herein, for the first time, free GA, two GA-dendrimers, and the dendrimer adopted as GA reservoir were tested on both sensitive and chemoresistant NB cells. The dendrimer device, administered at the dose previously found active versus sensitive NB cells, induced ROS-mediated death also in chemoresistant cells. Free GA proved a dose-dependent ROS-mediated cytotoxicity on both cell populations. Intriguingly, when administered in dendrimer formulations at a dose not cytotoxic for NB cells, GA nullified any pro-oxidant activity of dendrimer. Unfortunately, due to GA, nanoformulations were inactive on NB cells, but GA resized in nanoparticles showed considerable ability in counteracting, at low dose, ROS production and oxidative stress, herein induced by the dendrimer.

The aim of this study was to investigate the potential of retrieving polyphenolic antioxidants directly from wet pomegranate marcs: the fresh by-products obtained after pomegranate juice processing. These by-products mainly consist of internal membranes (endocarp) and aril residues. Even if they are still edible, they are usually discharged during juice production and, thus, they represent a great challenge in an eco-sustainable industrial context. Green technologies, such as ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), have been employed to convert these organic residues into recycled products with high added value. UAE and MAE were used both in parallel and in series in order to make a comparison and to ensure exhaustive extractions, respectively. Water, as an environmentally friendly extraction solvent, has been employed. The results were compared with those ones coming from a conventional extraction. The most promising extract, in terms of total polyphenol yield and radical scavenging activity, has been tested both as a potential natural additive and as a functional ingredient after its incorporation in a real food model and in a real cosmetic matrix, respectively. This study represents a proposal to the agro-alimentary sector given the general need of environmental “responsible care”.

Ellagic acid (EA) is a bioactive natural polyphenol with antioxidant and several other beneficial properties, but unfortunately it cannot be exploited for in vivo therapeutic applications because of its poor water solubility and accordingly low bioavailability. Aiming to increase EA solubility, an EA solid microdispersion was realized by employing only water and low methoxylated pectin as a food compatible excipient by applying spray drying technology. It showed a 22% (w/w) Drug Loading (DL), a 30 times improved water solubility and remarkable radical scavenging activity. Subsequently, adopting non-PAMAM hydrophilic and amphiphilic dendrimers as nanocontainers, two EA nanodispersions were achieved (60–70 nm) with 46 and 53% (w/w) DL, water solubility 300 to 1000 times higher than that of free EA and good antioxidant capability. In both cases, toxic agents such as castor oil, Tween 80 or the extensively used but not so safe PEG400 were avoided and no previously used carriers, including β-cyclodextrins, were adopted. The prepared micro- and nanodispersions showed water solubility never obtained before and represent non-toxic EA reservoirs suitable for food and biomedical applications.

FINNOVER is an EU Interreg-Alcotra project that aims to bring new perspectives to floriculture enterprises by recovering useful bioproducts from the waste produced during processing of several aromatic species. In this study, a new operation strategy to recover lavender (Lavandula angustifolia Mill.) solid by-products remaining after the extraction of the essential oil was developed. Pulsed ultrasound-assisted extraction was employed as a sustainable and eco-compatible technology to extract, in a very short time (10 min), this agricultural waste using a food-grade solvent (a mixture of ethanol/water). All the extracts obtained from both flower and leaf waste and flower-only residues, exhibit a promising total phenolic content (38–40 mg gallic acid/g of dry waste), radical scavenging activity (107–110 mg Trolox/g of dry waste) and total flavonoid content (0.11–0.13 mg quercetin/g of dry waste). Moreover, the chromatographic analysis of these extracts has shown that this overlooked agriculture waste can represent a valuable source of multifunctional compounds. Particularly, they exhibit a content of polyphenols and flavonoids up to 200 times higher than the corresponding leachate, and they are a valuable source of gentisic acid (1.4–13 mg/g dry waste) representing a new low-cost ingredient usable in different fields (i.e., cosmetic).