Global supply chains are facing many changes after the COVID-19 pandemic. This change impacts the way each supply chain measures its key performance indicators and determinants for its competitiveness. Aquaculture supply chains (SCA) play an essential role in global trading and fluctuated significantly during the COVID-19 pandemic when many aquaculture supply chains from emerging countries faced disruption. Therefore, after the COVID-19 pandemic, these supply chains have changed their measures and determinants to improve global competitiveness. This paper examines the change in this measure and determinants of the aquaculture supply chain in Vietnam, one of the world’s top 10 biggest exporting countries of aquaculture products. The paper reviews the list of measures and determinants of the aquaculture supply chain before and after the COVID-19 pandemic from the literature. It forms the framework under the shade of Resource-Based View theory. A total of 38 interviews with managers and CEOs of 36 enterprises and two government agencies in the aquaculture supply chain in Vietnam were conducted to explore the strategic changes in the measures of determinants to cope with the new circumstances of current global trade. The findings contribute to enriching the theory in the new VUCA (volatility, uncertainty, complexity, and ambiguity) business environment after the COVID-19 pandemic. In this context, SCA should be defined by supply chain virtues that are associated with the new business environment, such as SC resilience, SC sustainability, SC reliability, SC integration, etc. This finding implies a new horizon for RBV applications, highlighting their adaptability. It suggests that the measures defining competitive advantage in the new business environment should extend beyond the traditional tangible and intangible resources under business certainty. They should also encompass those that differentiate the firms and their supply chain during business turbulence.