Currently, there is a great interest in the research of basidial fungi as ligninolytic enzymes producers capable of plant waste degradation. These properties are promising in composite materials creation, which can be employed as bio-packaging, bioplastics, and building materials. The purpose of the work was to study the possibility of using sunflower seed hulls for the cultivation of lignin-destroying fungi for the further development of composite biodegradable materials based on the obtained products. For that point, Trametes polyzona (Pers.) Justo (2011) and Trichaptum abietinum (Dicks.) Ryvarden (1972) growth on solid nutrient media based on sunflower seeds hulls were explored. It was shown that the fungi cultivation duration of the Trametes polyzona is 10 days and of the Trichaptum abietinum KS10 is 7 days. The suitability of the obtained products for use in the composition of biodegradable composite materials was assessed visually. The most durable structure of the packaging composite was obtained on nutrient media based on milled sunflower seeds hulls with the oat bran addition.

The study of new strains of basidiomycetes as sources of biologically active substances is a promising direction in modern biotechnology. This work aims to isolate new cultures of the fungi Daedaleopsis tricolor, Pycnoporellus fulgens and Trichaptum abietinum from natural fruiting bodies and to improve their growth conditions on solid nutrient media. The identification of fungi was performed based on their morphological features and using the Sanger sequencing method. Cultivation was carried out by placing inoculum in the middle of a Petri dish and at the edge, which provided a more comprehensive definition of the characteristics of colonies and fungus hyphae. New strains were registered in Genbank Overview. The optimal cultivation temperature was 27 °C for all studied strains. The highest radial growth was observed on synthetic medium for D. tricolor (5.26 mm/day) and T. abietinum (7.5 mm/day), and on synthetic medium with lignin for P. fulgens (2.98 mm/day). The biomass amount of D. tricolor KS11 was 133.25 mg at 9 days of cultivation, that of P. fulgens KS12 was 86.73 mg at 16 days, and that of T. abietinum KS10 was 227.33 mg at 6 days. New strains of fungi can be used to obtain biologically active substances for the food, pharmaceutical and cosmetic industries.

Влияние состава среды на рост мицелия и биосинтез метаболитов Daedaleopsis tricolor