Bio-organic fertilizers can improve soil health and maintain microbial activity. The application of Aspergillus spp. can degrade organic matter, promote plant growth, and improve soil health by accelerating soil nutrients and biological activity. Previously, Aspergillus spp. has shown potential in phosphate solubilization and siderophore production. Still, a study gap remains, and focus has been placed on clarifying the impact of Aspergillus sp. AS2022 on soil microbial biomass and soil nutrient acceleration. For this purpose, a pot experiment was established with four different treatments: cow compost (CC), cow compost + AS2022 (CA), AS2022 (AS) strain only and control-only soil (CK). The amount of cow compost used was 180 mg N kg^-1. Results revealed that inoculation of AS2022 with cow compost stimulated nitrogen mineralization and enhanced available nitrogen and accelerated the soil enzyme activities, which proposed that it could contribute to wheat crop production through the initial and later phases of plant growth. Although a single application of AS2022 enhances plant growth compared to the control, it might be the release of secondary metabolites. However, our short-term findings showed that the combined application of beneficial fungal strain AS2022 along cow compost was suitable culture for wheat crop production and improvement of soil quality through organic matter degradation and accelerating soil nutrients.

| Mono-cropping agricultural activities and overuses of chemical-based fertilizers result in the deterioration of soil health and considerable economic loss. However, previous studies suggested that organic fertilizers can sustain soil health through improved soil microbial activities. Nevertheless, the effect of poultry compost and poultry fresh manure on P (Phosphatase) and C ( β -glycosidase) related enzymes activities, nitrogen mineralization, and fungal biomass in the mono-cropping soil are poorly understood. Though, the efforts were made to reveal how changes in the soil nitrogen mineralization, enzyme activities and fungal biomass influence plant growth. An incubation and pot experiments were carried out to investigate the effect of organic wastes [Poultry compost (PC), Poultry fresh manure (PFM), Chemical fertilizer (CF), and only soil (S)] was used at the quantity of 200 mg N kg -1 . We observed that soil amended with PC have produced more phosphatase and β -glycosidase enzyme activities and nitrogen mineralization (36.96 mg kg -1 ), indicating that it could be contributing to plant growth promotion. The fungal biomass increased after applying poultry compost, it also might be contributing to plant growth promotion. Therefore, poultry compost was a more suitable waste for mono-cropping soil systems than poultry fresh manure in soil enzyme activities, nitrogen mineralization, and fungal biomass. In conclusion, functional organic waste as an additive to conventional fertilization could save chemical fertilizer’s contribution and minimize soil deterioration by overuse of chemical fertilizers.