Emerging foodborne pathogens are challenging subjects of food microbiology with their antibiotic resistance and their impact on public health. Campylobacter jejuni, Salmonella spp. and Verotoxigenic Escherichia coli (VTEC) are significant emerging food pathogens, globally. The decrease in supply and increase in demand lead developed countries to produce animal products with a higher efficiency. The massive production has caused the increase of the significant foodborne diseases. The strict control of food starting from farm to fork has been held by different regulations. Official measures have been applied to combat these pathogens. In 2005 EU declared that, an EU-wide ban on the use of antibiotics as growth promoters in animal feed would be applied on 1 January 2006. The ban is the final step in the phasing out of antibiotics used for non-medical purposes. It is a part of the Commission's strategy to tackle the emergence of bacteria and other microbes resistant to antibiotics, due to their overexploitation or misuse. As the awareness raises more countries banned application of antibiotics as growth promoter, but the resistance of the emerging foodborne pathogens do not represent decrease. Currently, the main concern of food safety is counter measures against resistant bugs.
This study was conducted to identify microplastics (MPs) in drinking water from various sources in İstanbul that are known to pose potential health risks. One hundred drinking water samples were analysed. Samples were filtered with a glass filter (Ø: 1.0 μm). After filtration, microscopy was used, followed by SEM-EDS and ATR-FTIR identification to characterise MPs. Two shapes (fibers and fragments) and eight polymer types of MPs (ethylene propylene, neoprene, polyethylene, polyethylene terephthalate, polypropylene, polyvinyl chloride, polytetrafluoroethylene, vinyl chloride vinyl acetate copolymer) with sizes of 12–4892 µm (548 ± 777 µm) were detected. These MPs abundances ranged from 10 to 390 MP L−1 (134 ± 93 MP L−1). In the identification of MPs detected in filters by FTIR spectroscopy, bisphenol A, which is used in the production of various plastics and described as an important public health problem, was detected in 9.74% of MPs. Within the scope of the Sustainable Development Goals, UNEP has a specific objective of ensuring access to safe, affordable drinking water (SDG 6). With a clear statement, it should be emphasised that MPs are a significant barrier to the provision of safe drinking water, and a comprehensive plan for overcoming this barrier should be developed.
Bio-nanomaterials are gaining increasing attention due to their renewable and eco-friendly characteristics. Among these, nanocrystalline cellulose (NCC) stands out as one of the most advanced materials for applications in food, healthcare, composite production, and beyond. In this study, NCC was successfully extracted from cotton-based textile waste using a combination of chemical and mechanical methods. The cellulose fibers were first hydrolyzed using a dilute HCl solution, neutralized, and then dried, resulting in microcrystalline cellulose (MCC) with diameters ranging from 7 to 15 µm and lengths up to 300 µm (as observed via optical microscopy and scanning electron microscopy, SEM). To achieve nanoscale dimensions, NaOH/urea solution with mechanical treatment was applied, resulting in the successful extraction of NCC in the supernatant, particularly under room-temperature conditions. Dynamic light scattering (DLS) analysis confirmed the presence of nanostructures (average sizes ranging from 120 nm to 750 nm), and atomic force microscopy (AFM) analysis verified the nanoscale range (diameters between 2 and 4 nm and lengths from 200 nm to 1 µm). Fourier transform infrared (FTIR) spectroscopy revealed the conversion of cellulose I to cellulose II, confirming the successful transformation into NCC. For the first time, NCC was obtained from undyed cotton textile wastes using NaOH/urea treatment after HCl hydrolysis, eliminating the need for pre-treatment and intermediate steps.
1 October 2019 in Journal of Microbiological Methods
Background/aim: Ankaferd hemostat (ABS; Ankaferd blood stopper, Istanbul, Turkey) is a folkloric medicinal plant extract. The aim of this study was to determine the effect of Ankaferd hemostat (ABS) on the fate of Helicobacter pylori strains. The study also aims to determine alterations in the antimicrobial resistance of three different H. pylori strains in response to ABS exposure. Materials and methods: H. pylori Strain 1 was obtained from the culture collection ATCC 43504 and passaged three times for viability. Strain 2 was isolated from a gastric ulcer patient and Strain 3 was isolated from a gastritis patient. 1% of ABS was added to all of the strains and antimicrobial susceptibility was observed on 30 and 60 min after application. Results: The efficacy of ABS solutions in achieving significant logarithmic reduction in foodborne pathogens of H. pylori was observed in this study. This study showed that ABS has antibacterial (Anti-H. pylori) effects. Conclusion: Our present study indicated, for the first time, that ABS could act against H. pylori. ABS is clinically used for the management of GI bleeding due to benign and malignant GI lesions. Thus, the possible anti-H. pylori effect of ABS shall expand the therapeutic spectrum of the drug in GI lesions in relation to H. pylori infection such as peptic ulser disease (PUD) and lymphoid tissue ( MALT) lymphomagenesis.