Infectious diseases, the second leading cause of death worldwide, have traditionally been treated with antimicrobials. However, the emergence of drug-resistant microorganisms has driven the need for alternative therapies. This study aimed to assess the antibacterial efficacy of Capparis spinosa crude extracts and five essential oils (EOs) derived from Salvia officinalis, Eucalyptus globulus, Micromeria barbata, Origanum vulgare, and Juniperus excelsa. The EOs were extracted using hydro-distillation, and C. spinosa extracts were obtained using ethanol and acetone solvents. Microdilution assays revealed that O. vulgare EO exhibited the strongest activity against Listeria monocytogenes, Escherichia coli, Salmonella spp., and Brucella melitensis, while C. spinosa demonstrated significant antibacterial effects against L. monocytogenes and notable inhibition of Pseudomonas aeruginosa. The combination of EOs with antibiotics, including M. barbata, J. excelsa, S. officinalis, and E. globulus, enhanced the efficacy of the antibiotics against recalcitrant bacterial strains. The synergistic effects were evaluated through Fractional Inhibitory Concentration Index (FICI) analysis. These findings confirm that the antibacterial efficacy observed in the tested EOs, especially when used in synergy with antibiotics, offers a promising therapeutic strategy to combat antimicrobial resistance.

Background: The nasopharyngeal carriage of Streptococcus pneumoniae can be the source of transmission between humans and the starting step towards invasive pneumococcal diseases. Data on the carriage of pneumococci in children before and after the pneumococcal conjugate vaccines (PCV) integration in a country are essential for monitoring any change in pneumococcal carriage serotypes and their antimicrobial-resistance profiles. Methods: We investigated the epidemiology of S. pneumoniae carriage among children younger than five years old in Tripoli, Lebanon, in 2016, the same year of integration of PCV13 in the country’s Expanded Program on Immunization. Results: Of 104 participating children, 57 (54.8%) gave a positive culture for S. pneumoniae. Antimicrobial susceptibility testing revealed that 26.3% of isolates were multidrug-resistant. Resistance was detected mainly against oxacillin (77.2%), tetracycline (29.8%), erythromycin (22.8%), trimethoprim-sulfamethoxazole (22.8%), clindamycin (19.3%), minocycline (19.3%), and teicoplanin (1.8%). Serotyping analysis identified 14 distinct serotypes, with only 31.3% and 50% of isolates corresponding to vaccine serotypes covered by PCV13 and PCV20, respectively. The most common serotypes were 11A, 19F, 23A, and those of serogroup 24 (Sg24) accounted for 37.5% of the serotyped isolates. Conclusions: Our findings have revealed the circulation of a pool of pneumococci isolates with high levels of antibiotic resistance and different degrees of likelihood of causing invasive diseases in children under five years old in Tripoli in 2016. The overall limited PCV13 vaccine coverage in this study highlighted the need for vaccines with greater coverage in the immunization programs in Lebanon. Longitudinal national studies investigating the carriage of pneumococci in children are required to further assess the impact of the PCV vaccine on pneumococci carriage in children and steer new vaccine development.