Alfalfa (Medicago sativa) has strong stress resistance, high nutritional value, good palatability for cattle, high yield and a drought tolerance mechanism, but long-term planting leads to soil desiccation. This research was carried out to examine the soil water conditions of alfalfa pastures with different planted ages, and determine the optimum time for alfalfa rotation in a plateau area of a sub-alpine monsoon climate. Soil water depletion, soil compaction and vegetation characteristics of alfalfa pastures of different ages (i.e., two, four and seven years) were assayed and compared with those of a cornfield which served as the control crop. Three 20 × 20 m plots and five random quadrats per plot were established at each field. Soil water contents at 0–400 cm depth and plant biomass were compared among different vegetation types, soil transects and planting years. The results showed that at the 250–400 cm depth, the soil water storage of the four- and seven-year-old alfalfa pastures was much lower than in the two-year-old alfalfa pasture and in the cornfield. Moreover, the degree of soil water storage deficit of the four- and seven-year-old alfalfa pastures was much higher than in the other fields. Soil compaction of alfalfa pastures increased with increasing planting age and reached a peak value in the seven-year-old alfalfa pasture. The highest above-ground biomass was observed in the four-year-old alpine alfalfa pasture. Thus, the best cultivation period for alfalfa pastures was four years from the perspective of higher yield and lower soil water consumption in pastoral sub-alpine areas. This study provided a basis for sustainable alfalfa pasture cultivation, timely harvest, rotation and water management measures to be implemented in alpine grazing lands.

Burrowing animals are a critical driver of terrestrial ecosystem functioning, but we know little about their effects on soil microbiomes. Here, we evaluated the effect of burrowing animals on microbial assembly processes and co-occurrence patterns using soil microbiota from a group of habitats disturbed by Plateau pikas (Ochtona curzoniae). Pika disturbance had different impacts on bacterial and fungal communities. Fungal diversity generally increased with patch area, whereas bacterial diversity decreased. These strikingly different species–area relationships were closely associated with their community assembly mechanisms. The loss of bacterial diversity on larger patches was largely driven by deterministic processes, mainly due to the decline of nutrient supply (e.g., organic C, inorganic N). In contrast, fungal distribution was driven primarily by stochastic processes that dispersal limitation contributed to their higher fungal diversity on lager patches. A bacterial co-occurrence network exhibited a positive relationship of nodes and linkage numbers with patch area, and the fungal network presented a positive modularity–area relationship, suggesting that bacteria tended to form a closer association community under pika disturbance, while fungi tended to construct a higher modularity network. Our results suggest that pikas affects the microbial assembly process and co-occurrence patterns in alpine environments, thereby enhancing the current understanding of microbial biogeography under natural disturbances.

Alpine grasslands are a critical component of the Qinghai–Tibet Plateau ecosystem, but their soil seed bank (SSB) patterns and driving mechanisms remain unclear under the influence of climate change and human activities. This study analyzed grazing exclusion (via fencing) and grazing effects using 12 sites in the alpine steppe (AS) and alpine desert steppe (AD) in northern Tibet to analyze the effects of fencing and grazing management, as well as hydrothermal and soil factors, on the SSB density and diversity. Linear regression models were applied to explore the relationships between the SSB density and environmental factors, while comparisons of the management modes revealed the potential impacts of fencing. The results show that fencing significantly increased the SSB density and diversity, especially in the AS, while grazing negatively impacted the SSB density and the Pielou evenness index. Hydrothermal factors strongly influenced the SSB in the AS, with the density positively correlated with precipitation and negatively with temperature, while responses in the AD were weak. Soil factors, such as the available phosphorus (SAP) and available potassium (SAK), were key to SSB formation in the AD, whereas ammonium nitrogen (NH4_N) and the pH were critical in the AS. Fencing optimized the hydrothermal conditions and nutrient availability, promoting SSB recovery, though its effects varied between the grassland types. This study provides scientific insights for alpine grassland restoration and sustainable management.