Microbial communities in the intestinal tract are suggested to impact the ethiopathogenesis of Alzheimer’s disease (AD). The human microbiome might modulate neuroinflammatory processes and contribute to neurodegeneration in AD. However, the microbial compositions in AD patients at different stages of the disease are still not fully characterized. We used 16S rRNA analyses to investigate the oral and fecal microbiota in patients with AD and mild cognitive impairment (MCI) (n=84), at-risk individuals (APOE4 carriers) (n=17) and healthy controls (n=50) and investigated the relationship of microbial communities and disease specific markers via multivariate- and network-based approaches. We found a slightly decreased diversity in the fecal microbiota of AD patients (average Chao1 diversity for AD = 212 (SD=66); for Controls = 215 (SD=55)) and identified differences in bacterial abundances including Bacteroidetes, Ruminococcus, Sutterella, and Porphyromonadaceae. The diversity in the oral microbiota was increased in AD patients and at-risk individuals (average Chao1 diversity for AD = 174 (SD=60), for at-risk group = 195 (SD=49)). Gram-negative pro-inflammatory bacteria including Haemophilus, Neisseria, Actinobacillus and Porphyromonas were dominant oral bacteria in AD and MCI patients and the abundance correlated with the cerebrospinal fluid (CSF) biomarker. Taken together, we observed a strong shift in the fecal and the oral communities of patients with AD already prominent in prodromal and, in case of the oral microbiota, in at-risk stages. This indicates stage-dependent alterations in oral and fecal microbiota in AD which may contribute to the pathogenesis via a facilitated intestinal and systemic inflammation leading to neuroinflammation and neurodegeneration.

Background Diagnosis of Parkinson’s disease and atypical parkinsonism is based on clinical evaluation of the patient’s symptoms and on magnetic resonance imaging (MRI) of the brain, which can be supplemented by nuclear medicine techniques. MRI plays a leading role in the differentiation between Parkinson’s disease and atypical parkinsonism. While atypical parkinsonism is characterized by relatively specific MRI signs, imaging of Parkinson’s disease previously lacked such signs. However, high-field MRI and new optimized MRI sequences now make it possible to define specific MRI signs of Parkinson’s disease and have significant potential regarding differentiated imaging, early diagnosis, and imaging of disease progression. Methods PubMed was selectively searched for literature regarding the definition and discussion of specific MRI signs of Parkinson’s disease, as well as the most common types of atypical parkinsonism with a leading motor component. No time frame was set, but the search was particularly focused on current literature. Results This review article discusses the different MRI signs of Parkinson’s disease, multiple system atrophy, and progressive supranuclear palsy. The pathogenesis of the MRI signs is described, and imaging examples are given. The technical aspects of image acquisition are briefly defined, and the different signs are discussed and compared with regard to their diagnostic significance according to current literature. Conclusion The MRI signs of Parkinson’s disease, which can be defined with high-field MRI and new optimized MRI sequences, enable differentiated structural image interpretation and consecutive diagnostic workup. Despite the fact that the signs are in need of further validation by bigger studies, they have the potential to achieve significant diagnostic relevance regarding the imaging of Parkinson’s disease and atypical parkinsonism. Key Points: Citation Format

Purpose In this in vitro study, the effectiveness and safety of four aspiration-based techniques for thrombectomy are evaluated for three types of thrombi in a flow model simulating the femoropopliteal segment. Material and Methods Red, white, and mixed thrombi were produced in a standardized manner and used to simulate occlusion of a superficial femoral artery using a pulsatile flow model. Four techniques were compared: aspiration alone, aspiration + stent retriever, exposing thrombus to laser by an excimer laser system and a laser catheter + aspiration, and aspiration + mechanical fragmentation by a separator. Rate of first-pass recanalization, embolic events, and number of embolized fragments > 1 mm were compared. Results Aspiration alone, stent retriever, laser, and separator differed in rates of first-pass recanalization (53.3%; 86.6%; 20%; and 100%) and embolic events (40%; 93.3%; 73.3%; and 60%). Number of embolized fragments was lowest with aspiration and higher with separator, laser, and stent retriever. Rates of first-pass-recanalization (75%; 75%; and 45%) and embolic events (65%; 60%; and 75%) differed for red, white, and mixed thrombi. The mixed thrombus caused the highest number of embolized fragments, which was particularly high using the stent retriever. Conclusion Additional use of mechanical techniques significantly enhances the effectiveness of thrombectomy but simultaneously provokes more embolism. Laser seems to negatively alter the structure of a thrombus and thus diminishes the effectiveness, while provoking embolism. All techniques had lowest effectiveness, but highest embolism with the mixed thrombus. This was particularly striking when a stent retriever was used with the mixed thrombus.

CT protocols that diagnose COVID-19 vary in regard to the associated radiation exposure and the desired image quality (IQ). This study aims to evaluate CT protocols of hospitals participating in the RACOON (Radiological Cooperative Network) project, consolidating CT protocols to provide recommendations and strategies for future pandemics. In this retrospective study, CT acquisitions of COVID-19 patients scanned between March 2020 and October 2020 (RACOON phase 1) were included, and all non-contrast protocols were evaluated. For this purpose, CT protocol parameters, IQ ratings, radiation exposure (CTDI_vol), and central patient diameters were sampled. Eventually, the data from 14 sites and 534 CT acquisitions were analyzed. IQ was rated good for 81% of the evaluated examinations. Motion, beam-hardening artefacts, or image noise were reasons for a suboptimal IQ. The tube potential ranged between 80 and 140 kV_p, with the majority between 100 and 120 kV_p. CTDI_vol was 3.7 ± 3.4 mGy. Most healthcare facilities included did not have a specific non-contrast CT protocol. Furthermore, CT protocols for chest imaging varied in their settings and radiation exposure. In future, it will be necessary to make recommendations regarding the required IQ and protocol parameters for the majority of CT scanners to enable comparable IQ as well as radiation exposure for different sites but identical diagnostic questions.

Acute stroke imaging includes native CT, CT-angiography (CTA), and CT-perfusion (CTP). CTP assesses the irreversibly damaged infarct core (IC), and the potentially salvageable penumbra (PEN) and distinguishes these from areas of healthy parenchyma (HA). However, it requires additional contrast agent and radiation. Spectral-CT (SCT) enables spectral imaging like e.g., iodine-density imaging, and we evaluated its potential in estimating IC and PEN using monophasic CTA data only. We analysed 28 patients with mediainfarction. CTP-analysis derived areas of IC, PEN and HA on infarction side, as well as their healthy hemisphere’s counterparts were transferred to CTA as Region of interest (ROI). Spectral measurements included Hounsfield-Units in monoenergetic maps (MonoE) at 40 keV, 70 keV, and 120 keV, plus iodine-density (ID) and electron-density (ED) values, totalling 2970 values. Unilateral absolute values and ratios to the healthy counterparts were evaluated. Visual infarct delineation on each map was also rated. In all spectral maps, the infarct areas could be distinguished from the healthy counterpart by absolute values (p < 0.05). IC, PEN and HA could be distinguished from each other by absolute values (p < 0.05) (except for ED), and by the ratio-value formed to the contralateral side (p < 0.05). Detection of IC and PEN were best possible in ID (IC (AUC = 0.9999, p < 0.0001); PEN (AUC = 0.9745, p < 0.0001)) and MonoE40 (IC (AUC = 0.9963, p < 0.0001); PEN (AUC = 0.9622, p < 0.0001)). Differentiation of IC and PEN was also best in ID (AUC = 0.93, p < 0.0001) and MonoE40 (AUC = 0.80, p < 0.0001). Similarly, visual delineation was best too in ID and MonoE40. Accordingly, IC and PEN can be detected and differentiated in monophasic CTA by using SCT-derived spectral maps like ID or MonoE40.

Objective: Thrombectomy of the aortoiliac segment remains a challenge for surgical and endovascular revision. This study aimed to evaluate the concept of percutaneous thrombectomy in patients with aortoiliac segment occlusions. Materials & Methods: Eighteen patients with aortoiliac occlusion who underwent percutaneous thrombectomy were retrospectively identified using the local picture archive and divided into the stent-graft (N = 10) and native vessels (N  = 8) groups. The procedure was performed by placing a 12–24 French sheath adjacent to the distal end of the occluded vessel segment. The occlusion was passed with a balloon catheter which was retracted after inflation, to deliver the thrombus into the sheath. Technical success (reperfusion of the vessel and no residual thrombus/stenosis < 30%), complications and primary arterial patency were assessed. Follow-up included computed tomography angiography and evaluation of the clinical situation via telephone. Results: Technical success was achieved in 38% (7/18) of patients after percutaneous thrombectomy alone and in 100% after additional procedures. The most common complication was peripheral embolism (44%, 8/18), which was treated successfully in all cases and was linked to a mismatch between the sheath and target vessel of ≥ 1 mm (P < .01). There were no significant differences in the incidence of complications between the two groups. Primary patency was 72% (13/18) with no significant difference between groups (P  = .94). Follow-up CT scans were available for 13/18 patients (72%), with a mean follow-up time of 270 ± 146 days. All patients were contacted via phone (follow-up time, 653 ± 264 days). Conclusion: Percutaneous thrombectomy appears to be effective for revascularization of the aortoiliac segment, both in stent-grafts and in native vessels. The most common complication is peripheral embolism; however, the risk may be reduced by choosing an adequate sheath size.