Physical and biological treatment technology are considered a highly feasible and economic way to treat slaughterhouse wastewater. To achieve the desired effluent quality for disposal or reuse, various technological options were reviewed. However, most practical operations are accompanied by several advantages and disadvantages. Nevertheless, due to the presence of biodegradable organic matter in slaughterhouse waste, anaerobic digestion technology is commonly applied for economic gain. In this paper, the common technologies used for slaughterhouse wastewater treatment and their suitability were reviewed. The advantages and disadvantages of the different processes were evaluated. Physical treatments (dissolved air floatation (DAF), coagulation–flocculation and sedimentation, electrocoagulation process and membrane technology) were found to be more effective but required a large space to operate and intensive capital investment. However, some biological treatments such as anaerobic, facultative lagoons, activated sludge process and trickling filters were also effective but required longer start-up periods. This review further explores the various strategies being used in the treatment of other wastewater for the production of valuable by-products through anaerobic digestion.

In this study, the performance of a laboratory scale upflow anaerobic sludge blanket (UASB) reactor operating at mesophilic temperature (35 °C) was examined. Cattle slaughterhouse wastewater (CSWW) was used as the main substrate. The total and effective volumes of the reactor were 8 L and 6 L, respectively. Twelve different organic loading rates (OLR) were applied and the performance was evaluated. The chemical oxygen demand (COD) removal efficiency was more than 90% during batch study. In the continuous study, COD removal was also approximately 90% at OLR 0.4 g/L d⁻¹ which subsequently dropped to below 50% when the loading rate increased to 15 g/L d⁻¹. Approximately 5 L/d of biogas was obtained with high methane concentration at stages VI and XI corresponding to OLR of 2 and 10 g/L d⁻¹, respectively. It was observed that the concentration of volatile fatty acids was low and that the alkalinity of the wastewater was sufficient to avoid acidification. Specific methane yields of 0.36 and 0.38 LCH₄/g COD added were achieved at OLR 7 and 10 g/L d⁻¹. A hydraulic retention time (HRT) of 1 day was sufficient to remove greater than 70% of COD which correspond to 89% methane concentration. Parameters like soluble COD, NH₃-N, pH, alkalinity, total suspended solid (TSS), fats, oil, and grease were also investigated. The results show that the UASB reactor could serve as a good alternative for anaerobic treatment of CSWW and methane production.

Anaerobic digestion is one of the most essential treatment technologies applied to industrial and municipal wastewater treatment. Membrane-coupled anaerobic bioreactors have been used as one alternative to the conventional anaerobic digestion process. They are presumed to offer the advantage of completely reducing or minimizing the volume of sludge and increasing biogas production. However, researchers have consistently reported different kinds of fouling that resulted in the reduction of membrane life span. Depending on the strength of the effluent, factors such as high suspended and dissolved solids, fats, oil and grease, transmembrane pressure (TMP) and flux were reported as major contributors to the membrane fouling. Moreover, extracellular polymeric substances (EPSs) are an important biological substance that defines the properties of sludge flocs, including adhesion, hydrophobicity and settling and have been found to accelerate membrane fouling as well. Extensive studies of AnMBR have been done at laboratory while little is reported at the pilot scale. The significance of factors such as organic loading rates (OLRs), hydraulic retention time (HRT), pH and temperature on the operations of AnMBRs have been discussed. Microbial environmental conditions also played the most important role in the production of biogas and the chemical oxygen demand (COD) removal, but adverse effects of volatile fatty acids formation were reported as the main inhibitory effect. Generally, evaluating the potential parameters and most cost effective technology involved in the production of biogas and its inhibitory effects as well as the effluent quality after treatment is technically challenging, thus future research perspectives relating to food to microorganism F/M ratio interaction, sufficient biofilm within the reactor for microbial attachment was recommended. For the purpose of energy savings and meeting water quality discharge limit, the use of micro filtration was also proposed.

Anaerobic digestion technology provides an alternative route for sustainable management of organic waste. In this study, the performance of the hybrid upflow anaerobic sludge blanket (HUASB) reactor consisting of synthetic grass media as attached growth surface was investigated for the treatment of cattle slaughterhouse wastewater under mesophilic (35 ± 1 °C) condition. After acclimatization with synthetic wastewater, the reactor was loaded up to OLR 10 g L⁻¹d⁻¹, corresponding to 20 g COD/L at a varying hydraulic retention time (HRT) of 24, 30, 36, 42, and 48 h. The system attained a maximum COD removal efficiency of 97% total suspended solids (TSS), volatile suspended solids (VSS), fats, oil, and grease (FOG), color removal, and turbidity were found as 97%, 284 mg/L, 79%, 78%, and 91% respectively. The biogas production after 48 h was found as 38 L/d, with about 85% methane and specific methane production of 0.24 LCH₄/gCOD_added. The ratio of alkalinity was 0.22, while ammonia nitrogen concentration reached a maximum of 839 mg/L at a steady state. Scanning electron microscopic (SEM) analysis revealed a predominance of Methanosarcina bacteria with the coccoidal shape at the end of the performance study. Therefore, the results of the experiment showed that increasing HRT significantly affects the performance of the system.

Cattle slaughterhouse wastewater (CSWW) with an average chemical oxygen demand (COD) and biochemical oxygen demand of 32,000 mg/L and 17,000 mg/L, respectively, can cause a severe environmental hazard if discharged untreated. Conventional upflow anaerobic sludge blanket (UASB) reactor is used in the treatment of slaughterhouse wastewater to meet the discharge standard limit of wastewater discharge set by the Department of Environment Malaysia (DOE). However, at higher loading rates the conventional systems are characterized by slow-growing microorganism resulting in long startup period, surface scum formation, and sludge washout. In this work, the performance of two laboratory scale (12 L) conventional (R1) and modified (R2) UASB reactors treating CSWW at mesophilic (36 ± 1 °C) condition were investigated. Both reactors were subjected to increasing organic loading rate (OLR) from 1.75 to 32 g L⁻¹ day⁻¹. The average COD, BOD₅, and TSS removal efficiencies were ˃90%, at an OLR between 1.75 to 5 g L⁻¹ day⁻¹. The study revealed that R1 drastically reduced to 50, 53, and 43% with increasing OLR until 16 g L⁻¹ day⁻¹, whereas R2 maintained 76, 77, and 88% respectively, under the same OLR. Sign of reactor instability was very much pronounced in R1, showing poorly active Methanosaeta spp., whereas R2 showed a predominantly active Methanosarcina spp.

Cattle slaughterhouses generate wastewater that is rich in organic contaminant and nutrients, which is considered as high strength wastewater with a high potential for energy recovery. Work was undertaken to evaluate the efficiency of the 12 L laboratory scale conventional and a modified upflow anaerobic sludge blanket (UASB) reactors (conventional, R1 and modified, R2), for treatment of cattle slaughterhouse wastewater (CSWW) under mesophilic condition (35 ± 1 °C). Both reactors were acclimated with synthetic wastewater for 30 days, then continuous study with real CSWW proceeds. The reactors were subjected to the same loading condition of OLR, starting from 1.75, 3, 5 10, 14, and 16 g L⁻¹d⁻¹, corresponding to 3.5, 6, 10, 20, 28, and 32 g COD/L at constant hydraulic retention time (HRT) of 24 h. The performance of the R1 reactor drastically dropped at OLR 10 g L⁻¹d⁻¹, and this significantly affected the subsequent stages. The steady-state performance of the R2 reactor under the same loading condition as the R1 reactor revealed a high COD removal efficiency of 94% and biogas and methane productions were 27 L/d and 89%. The SMP was 0.21 LCH₄/gCOD added, whereas the NH₃-N alkalinity ratio stood at 651 mg/L and 0.2. SEM showed that the R2 reactor was dominated by Methanosarcina bacterial species, while the R1 reactor revealed a disturb sludge with insufficient microbial biomass.