Chlorella vulgaris Beijerinck is a spherical, green alga belonging to the genus Chlorella and family Chlorellaceae. It has high nutritional value and shows multiple biological effects. Dietary supplements that contain extracts of C. vulgaris are sold in the form of tablets, capsules, powders, and aqueous solutions. To the best of our knowledge, this is the first study to determine the content of bioelements (zinc, iron, and magnesium), phenolic compounds, and lutein before and after incubation with artificial digestive juices from preparations containing C. vulgaris. In this study, we used commercial preparations in the form of powder and tablets. The samples were incubated in artificial gastric juice and then in artificial intestinal juice for 30 and 90 min. The contents of bioelements were determined by using the flame atomic absorption spectrometric method. Lutein and phenolic compounds were analyzed by high-pressure liquid chromatography. We also aimed to evaluate the quality of chlorella-containing formulations by using the methods described in the European Pharmacopoeia 8th edition. According to the results, the preparations containing C. vulgaris demonstrated the presence of phenolic compounds and lutein. Therefore, daily supplementation of preparations containing C. vulgaris substantiates its usefulness for humans. The qualitative composition of the examined organic substances and bioelements was found to be in accordance with the manufacturer’s declarations on the packaging containing C. vulgaris compared with the control samples; however, the contents of bioelements were found to be negligible after incubation with artificial digestive juices. This shows that the examined preparations containing C. vulgaris are not good sources of bioelements such as zinc, iron, or magnesium.

Background: Numerous studies indicate thatStaphylococcus aureus(S. aureus) colonizing the nasal cavity plays a role in the pathogenesis of allergic rhinitis (AR). This bacterium is able to produce a variety of toxins with superantigenic properties that can exacerbate allergic inflammation.Objective: The objective of the study was to evaluate the ability of polyvalent mechanical bacterial lysate (PMBL) to eliminateS. aureusnasal carriage in children with grass pollen‐induced AR.Methods: This randomized, double‐blind, placebo‐controlled study included 80 children aged 5–17 years with seasonal AR (SAR). At the randomization visit and after 12 weeks of the study, a swab was taken from the region of the middle nasal meatus. Standard microbiology culture and identification techniques were used to analyze the swab contents.Results: Nasal colonization byS. aureuswas confirmed in 29 children (42%), withMoraxella catarrhalisin three participants (4.4%). Physiological flora was detected in 37 children. No statistically significant differences were observed between the two measurement points in both the PMBL and placebo groups with respect to the number of patients whose nasal swab cultures showed a growth ofS. aureus(p = 1). Both groups also showed no significant changes in the mean number ofS. aureuscolonies in nasal swab cultures taken at baseline and after 12 weeks of the study (PMBL groupp = .41; placebo groupp = .16).Conclusion: Almost every second child with SAR isS. aureusnasal carrier. Sublingual administration of PMBL in children with grass pollen‐induced AR did not affectS. aureusnasal colonization. Therefore, PMBL should not be used for the eradication ofS. aureusfrom the nasal cavity.

The aim of the study was to investigate the modified release of a model substance, of tablets containing different types of Kollidon and particular additives. Additionally, the release kinetics and mechanism of prolonged release of certain tablet preparations were investigated. In this work, tablets containing different types of povidone (Kollidon CL, Kollidon 30, Kollidon SR and other excipients) were prepared by the direct compression technique. The results showed that tablets with fast disintegration and release should contain in their composition, Kollidon CL, lactose and Avicel, however, the use of β-CD instead of lactose or Avicel brings about a slight prolongation in the disintegration time of tablets and the release of an active substance. Furthermore, while other tablet compositions generated within this study must be considered as being prolonged release types, only two of these showed the best fitted mathematical models. The in vitro dissolution data reveal that the dissolution profiles of the two formulations, one containing Kollidon SR with the addition of Kollidon 30, and the second with HPMC K15M, Kollidon 30, Kollidon CL and lactose, best fitted the Higuchi model. Moreover, the release mechanism of these two formulations plotted well into Korsmeyer-Peppas, indicating a coupling of drug diffusion in the hydrated matrix, as well as polymer relaxation – the so-called anomalous transport (non-Fickian).

The search for new active plant ingredients is crucial for the development of innovative cosmetic products. Micellar extracts from plant raw materials are not yet widely popular in cosmetics; however, scientific reports suggest that this form of extract is superior to standard extracts due to its enhanced ability to solubilize active compounds, improve their stability, and facilitate better penetration into the skin. For this reason, our research focuses on an innovative in its applicative form micellar extract from common bean sprouts (Phaseolus vulgaris L.) with a favorable composition and promising biological activity. The aim of this study was to develop a cream formulation containing this extract and evaluate its effects using in vivo tests. Six formulations were assessed for their physicochemical properties, and a comparative analysis was conducted against a reference cream and placebo cream. For the in vivo efficacy tests, the cream, which exhibited optimal physicochemical properties and long-term stability, was selected and tested on a group of 45 volunteers. The evaluation utilized Multi-Probe Adapter Systems to compare the cream with the micellar extract, a placebo cream (cream without the extract), and a reference antiaging cream. Results demonstrated that the formulation with micellar extract exhibited superior moisturizing, antiaging, and skin-brightening properties compared to the control groups. After 12 weeks of application, the micellar extract cream improved skin hydration by 22.31%, while the placebo cream showed only a 3.52% increase, and the reference cream achieved a 13.96% improvement. The antiaging effect, assessed based on improvements in skin elasticity parameters (R2 and R5), showed increases of 13.30% and 12.33% for the micellar extract cream, compared to 8.5% and 2.32% for the placebo cream and 6.38% and 3.82% for the reference cream, respectively. In conclusion, the common bean sprouts micellar extract shows potential as an effective active ingredient for skin care products, highlighting its promising applications in the cosmetics industry.

The influence of excipients on the disintegration times of tablets and the release of papaverine hydrochloride (PAP) from tablets were studied. Ten different formulations of tablets with PAP were prepared by direct powder compression. Different binders, disintegrants, fillers, and lubricants were used as excipients. The release of PAP was carried out in the paddle apparatus using 0.1 N HCl as a dissolution medium. The results of the disintegration times of tablets showed that six formulations can be classified as fast dissolving tablets (FDT). FDT formulations contained Avicel PH 101, Avicel PH 102, mannitol, β-lactose, PVP K 10, gelatinized starch (CPharmGel), Prosolv Easy Tab, Prosolv SMCC 90, magnesium stearate, and the addition of disintegrants such as AcDiSol and Kollidon CL. Drug release kinetics were estimated by the zero- and first-order, Higuchi release rate, and Korsmeyer-Peppas models. Two formulations of the tablets containing PVP (K10) (10%), CPharmGel (10% and 25%), and Prosolv Easy Tab (44% and 60%) without the addition of a disintegrant were well-fitted to the kinetics models such as the Higuchi and zero-order, which are suitable for controlled- or sustained-release.

The present paper describes the preparation of a new type of ternary composite where pure silica gel or polysilsesquioxane was deposited on a polymer carrier loaded with a high dose of diclofenac sodium. The silica species were prepared by in situ gelation of the precursors, tetraethoxysilane (TEOS) or ethyltriethoxysilane (ETEOS), in the presence of an acidic catalyst in the vapour phase. The conducted studies (low temperature nitrogen sorption, XRD, SEM, EDX) reveal that the introduction of drug molecules, as well as silica species, significantly changes the internal structure of the host material. The total porosity of the ternary composites strongly depends on the type of applied silica precursor. Additionally, it is shown that the exposure of the TEOS-saturated or ETEOS-saturated solid dispersion of drug within the polymer to acid vapors is sufficient to cause the irreversible transformation of diclofenac sodium into sodium chloride and a derivative of phenylacetic acid. Furthermore, TEOS prevents the transformation of the drug into its acidic form more effectively than the ETEOS precursor. Finally, the in vitro release of the drug is demonstrated, which clearly indicates that after the embedding both of the silica species, the rate of drug release is modified and the degree of initial drug delivery is successfully diminished. The obtained data are analyzed using different kinetics models to give insight into the possibility of prolonged release of a drug and the probable mechanism of drug release from the investigated samples.