Purple corn pericarp, a processing waste stream, is an extremely rich source of phytochemicals. Optimal polyphenol extraction parameters were identified using response surface methodology (RSM) by combining a deep eutectic solvent (DES) and ultrasound-assisted extraction (UAE) method. After DES characterization, Plackett–Burman design was used to screen five explanatory variables, namely, time, Temp (temperature), water, Amp (amplitude), and S/L (solid-to-liquid ratio). The total anthocyanin concentration (TAC), total polyphenol concentration (TPC), and condensed tannin (CT) concentration were the response variables. After identifying significant factors, the Box–Behnken design was utilized to identify the optimal extraction parameters. The experimental yields under the optimized conditions of time (10 min), temperature (60 °C), water concentration (42.73%), and amplitude (40%) were 36.31 ± 1.54 g of cyanidin-3-glucoside (C3G), 103.16 ± 6.17 g of gallic acid (GA), and 237.54 ± 9.98 g of epicatechin (EE) per kg of pericarp, with a desirability index of 0.858. The relative standard error among the predicted and experimental yields was <10%, validating the robustness of the model. HPLC analysis identified seven phytochemicals, and significant antioxidant activities were observed through four distinct assays. Metabolomic profiling identified 57 unique phytochemicals. The UAE technique combined with DES can efficiently extract polyphenols from purple corn pericarp in a short time.

A study was conducted to validate a simulated traditional crust pepperoni pizza baking process to control Shiga toxin-producing Escherichia coli (STEC) and to determine the heat resistance characteristics of STEC in pizza dough. Pizza dough and pepperoni slices were inoculated with 7 strains STEC cocktail and baked at 500°F (260°C) for 12 min using a conventional kitchen oven followed by 15 min of ambient air cooling. The mean internal temperature of the pizza reached 209.32 ± 1.94°F by the end of 12 min of baking and dropped to 137.90 ± 2.88°F after the 15 min ambient air cooling. The a_w and pH of the traditional crust pizza did not alter significantly during the baking process. The STEC population decreased by >5 log CFU/g in traditional crust pizza after 12 min of baking. Where pepperoni slices were used as a source of STEC introduction, a reduction of >6.5 log CFU/g was observed. The D-values of STEC cocktail in pizza dough at 55, 58, 61°C were 49.5 ± 4.10, 15.3 ± 0.68, and 2.8 ± 0.31 min, respectively. The z-value of STEC was 4.8 ± 0.16°C. This study validated that a typical traditional crust pizza baking process with ~209°F internal temperature for at least 12 min will result in 5 log reductions in STEC population.

A validation study was conducted to investigate the effect of the English muffin baking process to control Salmonella contamination and to study the thermal inactivation kinetic parameters (D- and z-values) of Salmonella in English muffin dough. The unbleached bread flour was inoculated with 3 serovar Salmonella cocktail (Salmonella serovars viz., Newport, Typhimurium, and Senftenberg), and dried back to its preinoculated water activity levels with 7.46 ± 0.12 log CFU/g of Salmonella concentration. The Salmonella inoculated flour was used to prepare English muffin batter and baked at 204.4°C (400°F) for 18 min and allowed to cool at ambient air for 15 min. The English muffins reached 99 ± 0°C (211.96 ± 0.37°F) as their maximum mean internal temperature during baking. The pH and a_w of English muffin dough were 5.01 ± 0.01 and 0.947 ± 0.003, respectively. At the end of the 18-min baking period, the Salmonella inoculated English muffins recorded a more than 5 log CFU/g reduction on the injury-recovery media. The D-values of 3 serovar cocktails of Salmonella at 55, 58.5, and 62°C were 42.0 ± 5.68, 15.6 ± 0.73, and 3.0 ± 0.32 min, respectively; and the z-value was 6.2 ± 0.59°C. The water activity (a_w) of the English muffin crumb (0.947 ± 0.003 to 0.9557 ± 0.001) remained statistically unchanged during baking, whereas the a_w of the muffin crust decreased significantly (0.947 ± 0.003 to 0.918 ± 0.002) by the end of 18 min of baking. This study validates and documents the first scientific evidence that baking English muffins at 204.4°C (400°F) for 18 min acts as an effective kill step by controlling Salmonella population by >5 log CFU/g.

The objective of this study was to validate a simulated commercial baking process for traditional crust pepperoni pizza to inactivate Salmonella when contamination was introduced through inoculated flour and pepperoni slices. The unbleached flour and pepperoni slices were inoculated (separate studies) with a 3‐serovar Salmonella cocktail and dried back to their respective pre‐inoculation water activity level to achieve 6.14 and 6.84 log CFU/g, respectively. The inoculated traditional crust pizza was baked at 260°C (500 °F) for 12 min followed by 15 min of ambient cooling. In both cases, a >6 log CFU/g reduction in Salmonella population was achieved by the first 8 min of baking. The pH (5.23–5.25) and water activity (0.958 ± 0.001–0.938 ± 0.005) of the pizza in this study did not change significantly. The D‐values of 3‐serovar Salmonella cocktail in traditional crust pepperoni pizza dough were 23.2 ± 1.82, 7.50 ± 0.32, and 2.0 ± 0.15 min at 56°C, 59°C, and 62°C, respectively, with a z‐value of 5.7°C. The study validated that traditional crust pepperoni pizzas when baked at 260°C (500 °F) for at least 12 min will reduce Salmonella populations by ≥5 log CFU/g if prebaking contamination occurs via flour and/ or pepperoni.

Consumption of mycotoxin contaminated food is the major source of human exposure to mycotoxins, and there are almost no treatments for mycotoxicosis

A study was conducted to validate the plain bagel baking process as an effective kill-step in controlling Shiga toxin-producing Escherichia coli (STEC) in the event of pre-baking contamination originating from flour. Unbleached bread flour was inoculated with five strains of STEC and dried back to its original water activity levels. The inoculated flour was used to prepare the bagel dough, proofed, boiled for 2 min, and baked at 232.2 °C (450 °F) for 14 min mimicking the commercial manufacturing process. Additionally, water activity (a_w) and pH in plain bagels during baking, and thermal inactivation kinetics (D- and z-values) of STEC in plain bagel dough were studied. The results clearly demonstrated that baking plain bagels at 232.2 °C (450 °F) for 14 min will result in at least a >5 log reduction in the STEC population, thus providing an effective kill-step assuring the safety of the finished food products. The pH of plain bagels increased significantly from pre-proofed plain bagel dough to seven min into the baking process, reaching a final value of 5.83. The water activity of the crust and crumb portions of plain bagels was significantly different during the baking process. The D-values of STEC in plain bagels at, 56, 59, and 62 °C were 26.3 ± 1.55, 9.0 ± 0.27, and 2.50 ± 0.15 min with a z-value of 5.8 ± 0.16 °C.