Agroforestry home gardens are integrated arrangements of common vegetable plants near residences, resembling tropical forests. They cultivate multiple species to meet families’ basic needs, including food, medicine, and family activities. This study aimed to assess the composition and use of plant species in agroforestry home gardens in three municipalities in the western region of Pará. The study analyzed 119 home gardens in Belterra, Mojuí dos Campos, and Santarém in Pará, Brazilian Amazonia. These home gardens span peri-urban, urban, floodplain, indigenous, and tourist land zones. Data were collected through questionnaires and visits, surveying 5323 plants from 188 species and 62 plant families. The findings revealed that 80.5% of plants concentrated in just 18.6% of the species, with no significant difference in species per home garden across zones. Notably, food species, particularly fruit-bearing plants, dominated these home gardens. This indicates a potential emphasis on incentive programs and public policies. Most home gardens contained up to 17 plant species, while less than half included medicinal plants. Native and exotic species were equally represented, with native plants valued for shading benefits. Agroforestry home gardens are vital for conserving and cultivating food species. Their specific purposes influence species distribution and selection, showcasing their socioeconomic and environmental significance. Thus, recognizing and investing in these land-use systems is crucial to maintain and enhance regional benefits.