The risk of zoonotic parasitic infections is closely linked to direct and indirect interactions between animals and humans. The mutual coexistence of species in public spaces predisposes individuals to gastrointestinal parasitosis owing to various social and hygienic-sanitary factors. This study aimed to assess the risk of environmental contamination by gastrointestinal parasites in fecal samples collected from parks and public squares in 18 municipalities located in a microregion of the central plateau of Brazil, correlating the collection with the population size of each municipality. We collected 536 soil fecal samples from 117 randomly selected public areas across a 56,111.874 km² region. Eggs, cysts, and oocysts were detected using the Willis flotation technique and the Hoffman’s spontaneous sedimentation method. The Hoffman’s analysis revealed that 70.3% of the fecal samples were infected across 91.5% of the sampled areas. Identified parasites included the members of the family Ancylostomatidae (56.5%), Toxocara spp. (6.2%), Trichuris spp. (1.7%), Strongyloides spp. (0.2%), Dipylidium caninum (25.8%), Spirometra spp. (0.4%), Taeniidae (0.2%), Platynosomum fastosum (0.6%), Trematoda (0.2%), Giardia spp. (3.2%), Cystoisospora spp. (5.6%), Sarcocystis spp. (0.2%), and Entamoeba spp. (2.4%). The presence of positive fecal samples in public areas and municipalities correlated with populations of up to 10,000 inhabitants (p = 0.023). Areas contaminated with feces were 63.4% more likely to contain parasites than other areas (odds ratio 1.6336). Favorable environmental factors combined with inadequate sanitary management contribute to a high risk of environmental contamination, representing a significant zoonotic potential and highlighting the need for improved public health policies and preventive measures.

Objectives: To compare the Willis, Sheather, Faust, Ritchie and spontaneous sedimentation techniques, as well as their combinations, for the diagnosis of gastrointestinal parasites in domestic and semi‐domesticated felines. Materials and Methods: Coproparasitological analyses were conducted on domestic cats of both sexes and all age groups, including kittens, adults and senior cats, between August 2024 and August 2025. Upon receipt, they were refrigerated at 4°C and processed within a maximum of 24 hours using five different coproparasitological techniques. Results: One hundred and fifteen samples were positive for at least one parasite form, with diagnoses of Toxocara spp., Ancylostomatidae, Dipylidium caninum , Spirometra spp., Platynosomum fastosum , Giardia spp., Cystoisospora spp. and Entamoeba spp. The Faust technique presented a superior range of diagnosis for low‐density structures, whereas the Ritchie technique was effective for high‐density structures. However, neither technique achieved a rate of at least 70%. Sensitivity analysis of the combination of these techniques revealed that: the Ritchie with spontaneous sedimentation technique was suitable for diagnosing toxocariasis and platynosomiasis; the Willis with Ritchie technique was appropriate for diagnosing hookworms; the Willis with Faust technique was suitable for diagnosing giardiasis; and the Sheather with Faust technique was appropriate for diagnosing cystoisosporiasis. It was not possible to analyse the association with other parasites owing to their low prevalence. Clinical Significance: Coproparasitological techniques remain the primary tools for diagnosing gastrointestinal parasites in routine feline practice, and the veterinarian's clinical suspicion is fundamental for performing the most appropriate technique. Considering the zoonotic potential of several parasites and frequent neglect of feline parasitosis, accurate diagnostic strategies and preventive veterinary practices are fundamental for reducing transmission in animals and humans.

Tortoises, such as Chelonoidis denticulatu s, are described as hosts of many parasites, such as helminths and ticks of the genus Amblyomma , which are important vectors of rickettsial infection in Brazil. Additionally, the high consumption of meat from these animals in Acre results in a high risk of zoonotic outbreaks due to contact with the hosts and, consequently, associated pathogens. This study aimed to describe the parasitic fauna of C. denticulatus. Two sampling efforts were conducted: the capture of a sample population of tortoises at the Rio Acre Ecological Station (active search) and the collection of viscera from animals consumed in the municipality of Cruzeiro do Sul. Following active search and incidental findings, ectoparasite searches were performed, and fecal samples were collected for coproparasitological examination. After these procedures, the animals were released back into their habitat. In total, seven animals were collected, with ticks on various parts of their bodies. A total of 51 samples, identified as Amblyomma humerale , were sent for molecular analysis to search for Rickettsia (all negative). Eggs of helminths and protozoan cysts, such as Entamoeba spp., were found, indicating environmental contamination and a potential zoonotic risk. Viscera of 10 tortoises from Cruzeiro do Sul were analyzed to search for helminths, and the species Labiduris zschokkei , Chapiniella variabilis , and Haltrema spp. were observed. Considering that the sampled animals were free‐living, the analysis highlights the importance of maintaining environmental quality. With respect to cultural aspects, the consumption of wild animals in the western Amazon of Brazil is evident, and C. denticulatus is one of the most consumed species. The contact of these species with humans, in a consumption relationship, is considered a risk factor for the emergence of spillover events, and monitoring the pathogens associated with these species is crucial.

The pacarana (Dinomys branickii) is a typical rodent of the Amazonian biome crepuscular habits, feeding on fruits, leaves, and roots. However, studies on these animals, inhabiting behaviors, and their parasites are limited. This study aimed to report the parasites found in fecal samples and a dead specimen of D. branickii in the Brazilian Cerrado. In 2023, fecal samples from five animals were collected and examined using flotation and simple sedimentation techniques for the identification of parasitic eggs. In 2025, a necropsy was performed on a decreased animal. Fecal samples of all animals were positive for eggs of Strongyloides spp., with two cases of co-infection with Oxyuroidea eggs and one with Trichuris sp. eggs. The Wellcomia branickii found during necropsy is a specific helminth of the pacaranas gastrointestinal tract. The natural geographical range of D. branickii is in the Western Amazon. Its introduction in the Cerrado, although for conservation purposes, reinforces the potential for this translocated species to disseminate non-native parasites outside its natural range.

This report aims to describe a case of equine colic that resulted in the death of a male Thoroughbred colt, caused by intestinal rupture due to high parasitism of Parascaris equorum in Jataí, Goiás. The animal showed intense abdominal pain, but veterinary care was not requested in time, and the animal died before the veterinary team arrived at the property where the animal was located. The necropsy was performed with the aim of discovering the sudden death of the animal, in which free fluid was observed in the abdominal cavity, as well as characteristic of serofibrinous peritonitis and the presence of helminths that were later identified, through their morphological characteristics, as P. equorum. This nematode can cause diarrhea, intestinal obstruction or asymptomatic. In cases of intense parasitism, it can evolve into cases of intestinal rupture followed by peritonitis, a fatal condition in horses. We relate the rupture followed by peritonitis with the high presence of this ascarid. This report demonstrates that the differential diagnosis of acute abdomen with poisonous colic must be considered, highlights the importance of early diagnosis through coproparasitological exams, often neglected, which allow identification of the parasite, correct treatment, and points out the lethality of this parasitism.