Cell death, cell proliferation, cell differentiation, cell invasion and cell migration are the fundamental cellular processes which are dysregulated due to

The current study investigated comprehensive phytochemical profile through high resolution techniques like GCMS, GC×GC–TOF–MS, UHPLC–QTOF–MS, total phenolic content, total flavonoid content, antioxidant, and anticancer activities of Morchella esculenta oil using in vitro approaches. The results showed the identification of 19 (GCMS) 20 (UHPLC–QTOF–MS) and 68 (GC×GC–TOF–MS) phytochemical compounds belonging to diverse classes of compounds. Hexadecenoic acid, oleic acid, palmitic acid, linoleic acid, octanoic acid, C16 sphinganine, allopumiliotoxin267A, 3’-Hydroxy-T2 Toxin were the main compounds identified. The Morchella esculenta oil showed moderate phenolic content (13.26 ± 2.52 mg GAE/g dw), flavonoid content (11.35 ± 1.58 mg RE/g dw) and antioxidant activities (DPPH-IC50 = 118.46 µg/mL; ABTS-IC50 = 125 µg/mL; and FRAP-IC50 = 117.90 µg/mL). Using human cervix (HeLa), human colon (HCT-116) and human breast cancer (MCF-7) cell lines, essential oil of Morchella esculenta showed a dose/time-dependent antiproliferative and cytotoxicity activity with GI50 values of 27.61 µg/mL, 19.52 µg/mL, and 31.34 µg/mL respectively. The obtained results confirmed that Morchella esculenta oil is a rich source of bioactive molecules with strong antiproliferative and cytotoxicity potential towards cancer cell lines.