Thulium Iron Garnet (TIG), as an emerging hotspot in rare-earth iron garnet systems, possesses a large magnetostriction constant (λ₁₁₁) and a low damping coefficient. Therefore, it is possible to induce perpendicular magnetic anisotropy (PMA) through stress, which makes it more desirable for interfacial magnetic proximity or spin–orbit torque effects than Yttrium Iron Garnet (YIG). For achieving a high-quality TIG thin film and regulating its properties accordingly, understanding the effect of growth parameters on the film properties is essential. Using the Pulsed Laser Deposition (PLD) technique, we prepared TIG film on a Gadolinium Gallium Garnet (GGG) substrate. The correlations of its structural properties to the growth conditions are systematically studied, including the oxygen pressure and laser energy. With the annealing, a ferrimagnetic TIG thin film with PMA is successfully obtained. Our work provides a platform for achieving high-quality TIG thin films by experimentally regulating the growth factors.