Selenium and its derivatives have been found capable of excellent biological responses. However, the element in its bulk form has low bioavailability and increased toxicity, meaning the production of effective forms with sustainable methods has become urgent. Several microorganisms, including fungi, bacteria and yeast, as well as higher plants, are capable of biosynthesizing nanoparticles such as nano-selenium (nano-Se), which has wide applications in medicine, agriculture and industry. Thus, the biosynthesis of nano-Se using some bacterial species was the main target of this study. The production of nano-Se and the monitoring of its impact on the wheat germination of seeds under salt stress (i.e., 50, 100, and 150 mM NaCl) was also evaluated in the current study. The ameliorative role of nano-Se doses (i.e., 50, 75, and 100 mg L⁻¹) in the germination of wheat seeds under salt stress was also investigated. Based on sodium selenite tolerance and reducing selenite to elemental Se-NPs, the most effective isolate (TAH) was selected for identification using the 16S rRNA gene sequence, which belonged to Bacillus cereus TAH. The final germination percent, mean germination time, vigor index and germination rate index were improved by 25, 25, 39.4 and 11%, respectively, under 15 mM sodium chloride concentration when 100 mg L⁻¹ nano-selenium was used. On the other hand, the results obtained from a gnotobiotic sand system reveal that with treatment with 100 mg L⁻¹ nano-selenium under high Ec values of 14 ds m⁻¹, the vegetative growth parameters of shoot length, root length, fresh weight and dry weight were improved by 22.8, 24.9, 19.2 and 20%, respectively, over untreated controls. The data obtained from this study reveal that the use of nano-selenium produced by Bacillus cereus offers improved wheat seed germination under a salt-affected environment.

Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were Bacillus paramycoides MCCC 1A04098, Azotobacter salinestris NBRC 102611 and Brevundimonas naejangsanensis BIO-TAS2-2. The addition of sugar beet molasses to the medium of A. salinestris increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from B. paramycoides and A. salinestris showed similar bands which confirmed the presence of PHB; however, B. naejangsanensis showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for B. paramycoides and isopropyl ester of 2-butenoic acid for both of A. salinestris and Brevundimonas naejangsanensis. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics.

Sandy soil covers a significant portion of Egypt’s total land area, representing a crucial agricultural resource for future food security and economic growth. This research adopts the hypothesis of maximizing the utilization of secondary products for soil improvement to reduce ecosystem pollution. The study focuses on assessing the impact of combining phosphogypsum and modified biochar as environmentally friendly soil amendments on loamy sand soil quality parameters such as soil organic carbon, cation exchange capacity, nutrient levels, and wheat yield. The treatments were T₁: the recommended NPK fertilizer (control); T₂: 2.5 kg phosphogypsum m⁻² soil; T₃: 2.5 kg rice straw biochar m⁻² soil; T₄: 2.5 kg cotton stalk biochar m⁻² soil; T₅: 2.5 kg rice-straw-modified biochar m⁻² soil; T₆: 2.5 kg cotton-stalk-modified biochar m⁻² soil; and T₇ to T₁₀: mixed phosphogypsum and biochar treatments. The results revealed that the combined use of phosphogypsum and modified cotton stalk biochar (T₁₀) significantly enhanced soil organic carbon (SOC) by 73.66% and 99.46% in both seasons, the soil available N both seasons by 130.12 and 161.45%, the available P by 89.49% and 102.02%, and the available K by 39.84 and 70.45% when compared to the control treatment. Additionally, this treatment led to the highest grain yield of wheat (2.72 and 2.92 Mg ha⁻¹), along with a significant increase in straw yield (52.69% and 59.32%) compared to the control treatment. Overall, the findings suggest that the combined use of phosphogypsum and modified biochar, particularly cotton-stalk biochar, holds promise for improving loamy sand-soil quality and wheat productivity.