Rosmarinic acid is a well-known natural antioxidant and anti-inflammatory compound, and it is one of the polyphenolic compounds found in comfrey plants. Comfrey root also contains allantoin, which helps with new skin regeneration. This study aimed to investigate the healing and skin regeneration process of skin wounds in Wistar rats using creams based on comfrey extract and to correlate the results with active compounds in the extract. The obtained results showed that comfrey root is rich in bioactive compounds, including allantoin, salvianolic acid, and rosmarinic acid, which are known for their great free radical scavenging activity, and the high antioxidant activity of the extract may be mainly due to these compounds. The obtained extract has an antimicrobial effect on Staphylococcus aureus (1530.76/382.69), Escherichia coli (6123.01/6123.01), and Pseudomonas aeruginosa (6123.01/6123.01). The macroscopic evaluation and the histological analysis of the skin defects 14 days after the intervention showed faster healing and complete healing in the skin excisions treated with oil-in-water cream with 20% extract of comfrey as the active ingredient.

In the pathology of the urinary system, given its structural and functional complexity, the clinician must correlate the data obtained from the clinical and paraclinical examination. The aim of the study was to identify and apply the most relevant paraclinical methods for establishing the diagnosis of renal disease in dogs and to develop a reliable and easy to use working protocol. The biological material consisted of 25 dogs of different ages and sexes. The conventional and contrast-enhanced radiological examination together with the ecography, spectrofluorimetric analysis, urinary sediment examination, renal biopsy, cytological examination completed the disease information in order to establish a certitude diagnosis. In the management of patients with urinary disorders, the working protocol should include not only the animal anamnesis and a complete clinical examination but also a paraclinical examination which should be used depending on the patient’s clinical manifestations.

Assessing the behavior of a dog in a shelter is extremely important because the type of behavior can tell us if the animal can be given up for adoption, to whom it can be given up for adoption, if it cannot be given up for adoption and in which direction improvements in behavior need to be made to get it safely given up for adoption. This study aimed to assess the behavior of dogs in a public dog shelter by how a dog behaves towards a person (examiner) in a sequence of situations and to determine the number of individuals who responded with aggression. The behavior test was developed by The Rottweiler Rescue Society (Ontario, Canada) and John Rogerson, Blue Cross, Britain. Following minor modifications, has been used to examine dogs in public shelters. The test contains two additional assessment items from the article "An Evaluation of a Behavior Assessment to Determine the Suitability of Shelter Dogs for Rehoming" [1]. A total of 187 adult dogs were examined, of which 24 were identified with aggressive behavior and the types of aggression identified were dominance, interspecific, or territorial aggression. No behavioral test can show specifically how a dog will behave in a new environment, but the information obtained from the examination can indicate extreme manifestations of canine behavior, such as dominance aggression, possessive aggression, territorial aggression, and separation anxiety.

Diabetes is a chronic disease that severely impairs wound healing, slowing wound closure; thus, the risk of infection and increases the occurrence of other complications. The development of a suitable material that can accelerate the process of chronic wound regeneration, particularly in diabetic wounds, remains a significant challenge. In the present study, Sepigel 305^® paraffin-based oil-in-water cream containing spherical gold nanoparticles–bioactive glass was used in rats with induced diabetes mellitus. After wound closure, the stage of regeneration was evaluated histopathologically. It was shown that the wounds treated with the experimental product were closed macroscopically after 14 days, but the histological images still indicated an inflammatory process, suggesting incomplete deep dermal healing. Macroscopic closure of wounds treated with the studied cream after 14 days, which is a normal time for skin healing, represents a successful outcome in diabetic patients because the risk of bacterial infection is reduced, and thus the chance of complete healing increases.

Emotional contagion in human-horse interactions has been widely studied, yet the role of body language in stress transfer remains insufficiently explored. This study examines whether human emotional states, particularly stress and anxiety, influence horses and whether the transmission occurs primarily through body language. Conducted across five equestrian facilities, the study employed a within-subjects design, assessing 33 horse-human interactions. Participants were classified into high-anxiety (HA) and low-anxiety (LA) groups based on State Anxiety Scale scores and heart rate variability (HRV) measurements. Two interaction conditions were tested: Free-Style (FS), where participants moved naturally, and Constrained-Style (CS), where movement and expression were restricted. Horses heart rate variability and ethogram scores were recorded at baseline, during contact, and after recovery. Results indicated that horses exhibited significantly higher response in HRV and ethogram scores for HA-FS interactions compared to HA-CS (p < 0.05), suggesting an increased physiological and behavioral response when human emotions were freely expressed. However, interactions in the CS condition showed no significant HRV or behavioral differences between HA and LA groups, implying that constrained body language mitigates emotional contagion. These findings suggest that horses do not inherently detect human stress but rather respond to body language cues associated with anxiety. The study highlights the importance of structured handling techniques to minimize stress transfer, with implications for equestrian training and therapy. By adopting controlled, neutral body language, handlers can create a more stable environment, enhancing horse welfare and optimizing human-horse interactions. This research contributes to the broader understanding of interspecies emotional dynamics and the necessity of mindful equine management practices.