Life‐threatening sepsis with high mortality and morbidity is an important cause of acute kidney injury and myocardial dysfunction. In this study, we investigated the protective effect of Micromeria congesta (MC) against kidney and heart damage caused by lipopolysaccharide (LPS) used as a sepsis model. Control, LPS, LPS + 25 mg/kg MC and LPS + 50 mg/kg MC groups were established from rats for the study. After the experiment, kidney and heart tissues obtained from the rats were stained with hematoxylin‐eosin for histopathologic examination. Immunohistochemical staining was performed to determine inflammation, apoptosis, oxidative stress and DNA damage. IL‐2 for inflammation, CASP‐3 for apoptosis, HSP‐27 for oxidative stress and 8‐OHdG for DNA damage were used for immunopathologic examination. Histopathologic examination showed that the lesions in the kidney and heart tissues in the LPS group decreased with increasing doses of MC. Immunohistochemical examination showed that the expression of IL‐2, CASP‐3, HSP‐27 and 8‐OHdG was severe in the LPS group, but the severity of expression in these tissues decreased with increasing doses of MC. As a result of the study, it was histopathologically determined that MC reduced LPS‐induced kidney and heart tissue damage. In addition, MC was found to protect against LPS by reducing LPS‐induced inflammation, apoptosis, oxidative stress and DNA damage in kidney and heart tissue. In conclusion, it was seen that MC was effective in sepsis damage. However, it was concluded that MC could be an alternative in drug strategies developed for sepsis treatment with studies in vivo including more analyses.

This study aimed to investigate the possible histopathological and immunohistochemical effects of bovine colostrum (BC) against the toxic effects of 5-fluorouracil (5-FU) on the liver, kidney, and heart of Wistar Albino rats. Animals were divided into three groups: control, 5-FU, and 5-FU+BC. The control group received 2 mL/kg i.p. saline, the 5-FU group 100 mg/kg i.p. 5-FU, and the 5-FU+BC group received 100 mg/kg i.p. saline on the first day of the study. The 5-FU and 5-FU+BC groups received 100 mg/kg i.p. of 5-FU and 1000 mg/kg BC orally each day of the study. Liver, kidney, and heart tissues were examined histopathologically for lesions and the expression of TNF-α, HSP-27, CASP-3, and 8-OHdG. No pathologic lesions were observed in the control group, whereas severe pathologic lesions were observed in the 5-FU group. In the 5-FU+BC group, the lesions were less severe than in the 5-FU group. In immunohistochemical examination, biomarker expression was not observed in the control group, whereas it was severe in the 5-FU group and less severe in the 5-FU+BC group. At the end of the study, it was observed that 5-FU-induced pathological findings in liver, kidney, and heart tissues decreased with the use of bovine colostrum. The difference between the control group and the 5-FU and 5-FU+BC groups was significant (p < 0.01 and p < 0.05, respectively). Although the BC addition did not show any statistical significance in the pathological scores of 5-FU in liver, kidney, and heart tissues, it was observed that it improved the lesions of these tissues. Nevertheless, histopathological and immunohistochemical analyses showed visible improvements in the 5-FU+BC group. Although more studies are needed, it is hoped that BC will improve prognosis by both reducing the side effects of 5-FU, a good chemotherapeutic agent, and its antineoplastic properties.

In this study, the structure of the papillae in the tongue of Morkaraman sheep was investigated by light (LM) and scanning electron microscopy (SEM). The aim of this study was to investigate the morphology and topographic distribution of the lingual papillae of the tongues of Morkaraman sheep breed. Adult male Morkaraman sheep tongues were used in the study. Tongue samples for light and scanning electron microscopy were taken from the apex, corpus, radix and torus lingua regions of the upper surface of the tongue and the lower surface of the tongue tip. Two types of taste papillae, papillae fungiformis and vallata, were observed on the tongue. Papillae fungiformis were found under the tip of the tongue, on the upper surface and sides of the tip and body of the tongue and on torus lingua. Papillae vallata were observed on both posterior sides of the torus lingua of the tongue. SEM examination showed that the surface of the papillae was smooth and there were no taste pores opening to the surface. On LM examination, filiform papillae at the lingual apex were common in materials. These papillae had secondary keratin extensions on the main body. The light and scanning electron microscopic structures of the taste papillae on the tongue of Morkaraman sheep were examined and their similarities and differences with other ruminant species were determined.

Aim: In this study, it was aimed to investigate the effects of Gundelia tournefortii plant extract on the spleen of rats with experimental type of rats. Methods: In the study, 35 adult Wistar Albino rats were used. Thirty-five male rats were randomly divided into five equal groups of seven rats each. Animal groups were determined as Control, Diabetes, Diabetes + Treatment 1, Diabetes + Treatment 2, Diabetes + Treatment 3. The control group was not exposed to any treatment. The other groups received 45 mg/kg Streptozotocin intraperitoneally on the experimental day. Animals in Diabetes + Treatment 1, Diabetes + Treatment 2 and Diabetes + Treatment 3 groups were administered 50 mg/kg, 100 mg/kg and 200 mg/kg of Gundelia tournefortii extract daily by oral gavage for 21 days, respectively. At the end of the experiment, the animals were euthanized, and the spleen was properly removed. The tissues were stained with hematoxylin and eosin after routine tissue tracing. Results: Diabetes mellitus caused a decrease in the diameter of the white pulp and severe atrophy in the spleen tissue. In the red pulp, on the contrary, there was an increase in activation. In the treatment groups, it was determined that Gundelia tournefortii extract minimized the damage of diabetes and even the group with images close to the control group was Diabetes + Treatment 3, while in the other treatment groups, the rate of damage repair decreased as the dose decreased. Conclusion: In the study, it was determined that diabetes caused damage to the spleen tissue and these damages were reduced by Gundelia tournefortii plant extract. In addition, in the comparison between different doses of this plant extract in the treatment groups, it was determined that the best result was obtained in the group administered at the highest dose of 200 mg/kg. How to cite this article: Demircioğlu M, Dörtbudak MB. Protective effect of Gundelia Tournefortii extract on spleen tissue in experimental type I diabetes in rats. J Med Dent Invest 2023; 4: e230339. https://doi.org/10.5577/jomdi.e230339

Aim: The present study was conducted to determine the effects of pomegranate peel extract (PPE) and N-acetyl Cysteine (NAC) alone or in their treatment combinations with commercial preparation Ornipural® (ORN) on bone metabolism against experimentally cadmium (Cd)-induced toxicity in rats. Materials and Methods: Totally 36 animals were used in the study including 6 Wistar Albino rats in each group. The animals were assigned to control, Cd, Cd+PPE, Cd+NAC, Cd+PPE+ORN and Cd+NAC+ORN groups. The animals in the groups were euthanized after their blood samples were taken at the end of the 8th week. The bones were subjected to maceration for morphometric and histopathological examinations after euthanasia. Results: The statistically significant differences were determined between the treatment groups and Cd group in terms of histopathological changes (osteoporotic alterations and changes in red bone marrow) and biomarkers (Ca, P and Mg) (P<0.05). Conclusion: Although PPE, NAC and treatment combinations with ORN applied against experimentally induced cadmium toxicity were determined to have positive effects on bone metabolism, it has been thought that carrying out trials by increasing treatment duration and dose would be beneficial to determine definite efficacy of the applied treatment protocols.