Microplastics (MP) are widespread pollutants that pose a risk to soil ecosystems globally, especially in agricultural soils. This study introduces a method to extract and identify MP in Brazilian tropical soils, targeting debris of low-density polyethylene (LDPE) and polyvinyl chloride (PVC) polymers, commonly present in agricultural settings. The method involves removing organic matter and extracting MP using density separation with three flotation solutions: distilled water, NaCl, and ZnCl₂. Extracted MP are then analyzed through optical microscopy and Fourier transform infrared spectroscopy. The organic matter removal efficiency ranged from 46% to 89%, depending on the initial organic matter content in the soil. Recovery rates for LDPE ranged from 81.0% to 98.8%, while PVC samples showed a range of 59.7% to 75.2%. Finally, this methodology was tested in four agricultural raw soil samples (i.e., without any polymer enrichment) The values found in the soil samples were 2517.5, 2245.0, 3867.5, and 1725.0 items kg⁻¹, for ferralsol, nitisol, gleysol, and cambisol samples, respectively, with MP having diverse shapes including fragments, granules, films, and fibers. This approach lays the groundwork for future studies on MP behavior in Brazilian tropical agricultural soils.

The interaction of biofilms with metallic surfaces produces two biologically induced degradation processes of materials: microbial induced corrosion and bioleaching. Both phenomena affect most metallic materials, but in the case of noble metals such as gold, which is inert to corrosion, metallophilic bacteria can cause its direct or in direct dissolution. When this process is controlled, it can be used for hydrometallurgical applications, such as the recovery of precious metals from electronic waste. However, the presence of unwanted bioleaching-producing bacteria can be detrimental to metallic materials in specific environments. In this work, we propose the use of single-layer graphene as a protective coating to reduce Au bioleaching by Cupriavidus metallidurans, a strain adapted to metal contaminated environments and capable of dissolving Au. By means of Scanning Tunneling Microscopy, we demonstrate that graphene coatings are an effective barrier to prevent the complex interactions responsible for Au dissolution. This behavior can be understood in terms of graphene pore size, which creates an impermeable barrier that prevents the pass of Au-complexing ligands produced by C.metallidurans through graphene coating. In addition, changes in surface energy and electrostatic interaction are presumably reducing bacterial adhesion to graphene-coated Au surfaces. Our findings provide a novel approach to reduce the deterioration of metallic materials in devices in environments where biofilms have been found to cause unwanted bioleaching.

The assessment of microplastics (MPs) in terrestrial ecosystems has garnered increasing global attention due to their accumulation and migration in soils, which may have potential impacts on soil health, biodiversity, and agricultural productivity. However, research on their distribution and interactions in soil remains limited, especially in tropical regions. This study aimed to characterize MPs extracted from tropical soil samples and relate their abundance to soil and terrain attributes under different land uses (forest, grassland, and agriculture). Soil samples were collected from an experimental farm in Lavras, Minas Gerais, Southeastern Brazil, to determine soil physical and chemical attributes and MP abundance in a micro-watershed. These locations were also used to obtain terrain attributes from a digital elevation model and the normalized difference vegetation index (NDVI). The majority of microplastics found in all samples were identified as polypropylene (PP), polyethylene (PE), polyethylene terephthalate (PET), and vinyl polychloride (PVC). The spatial distribution of MP was rather heterogeneous, with average abundances of 3826, 2553, and 3406 pieces kg⁻¹ under forest, grassland, and agriculture, respectively. MP abundance was positively related to macroporosity and sand content and negatively related to clay content and most chemical attributes. Regarding terrain attributes, MP abundance was negatively correlated with plan curvature, convergence index, and vertical distance to channel network, and positively related to topographic wetness index. These findings indicate that continuous water fluxes at both the landscape and soil surface scales play a key role, suggesting a tendency for higher MP accumulation in lower-lying areas and soils with greater porosity. These conditions promote MP transport and accumulation through surface runoff and facilitate their entry into the soil.

Research on microplastics (MPs) in agricultural soils has received increasing attention due to their potential ecological risks and adverse effects on the food chain. Recently, geostatistical approaches have been increasingly used to assess the spatial distribution of MPs in soils. Therefore, this study aims to predict the abundance of MPs in the soil of an agricultural micro-watershed using geostatistical methods and to produce a continuous map of the interpolated MPs. Soil samples were collected, and MP abundance was determined using the density separation method. Subsequently, exploratory data analysis was conducted, followed by the construction of the experimental semivariogram, theoretical variogram model fitting, ordinary kriging interpolation, cross-validation and, inverse distance weighting (IDW) interpolation. MPs were detected in all samples, with average abundances of 3826, 2553, and 3407 pieces kg⁻¹ in forest, pasture, and agricultural land use systems, respectively. The experimental semivariogram showed that the spatial distribution of MPs has a weak spatial dependence structure. The Kriging and IDW maps showed a distribution of MPs in the range of 600 to 7400 pieces kg⁻¹, with higher concentrations of MPs for forest and agricultural areas. Additionally, the map reveals a high abundance of MPs, with greater concentrations in depressions and areas near roads and urban centers, allowing for identifying critical points within the micro-watershed. This study offers important insights into the presence of MPs across various land uses, emphasizing the need for proactive measures to prevent and mitigate their accumulation in soil.