Methicillin-resistant Staphylococcus aureus (MRSA) is a devastating global health concern. Hypervirulent strains are on the rise, causing morbidities and mortalities worldwide. In tertiary care hospitals, critically ill patients, those undergoing invasive procedures, and pediatric and geriatric patients are at risk. It is not fully clear how strains adapt and specialize in humans and emerge despite the well-established commonality of the S. aureus genome from humans and animals. This study investigates the influence of age-, gender-, and source-specific profiles (clinical, intensive care unit (ICU vs. non-ICU)) on the evolution of hospital-associated (HA)-MRSA versus community-associated (CA)-MRSA lineages. A total of 253 non-duplicate S. aureus isolates were obtained from May 2023 to March 2025. The patients were stratified by age and gender in ICUs and non-ICUs. Standard microbiology methods and Clinical and Laboratory Standards Institute (CLSI) guidelines were used for identification and susceptibility testing, with cefoxitin and oxacillin disk diffusions and molecular diagnosis confirming MRSA. Mann–Whitney U and Chi-square tests assessed the demographic distributions, clinical specimen sources, and MRSA/methicillin-sensitive S. aureus (MSSA) prevalence. Of 253, 41.9% originated from ICUs (71% male; 29% female) and 58.1% from non-ICU wards (64% male; 36% female). In both settings, MRSA colonized the two extremes of age (10–29 and 70+) for males and females, with different mid-life peaks or declines by gender. However, the overall demographic distribution did not differ significantly between the ICU and non-ICU groups (p = 0.287). Respiratory specimens constituted 37% and had the highest MRSA rate (42%), followed by blood (24.5%) and wounds (10.3%). In contrast, MSSA dominated wounds (20.3%). Overall, 73.9% were resistant to cefoxitin and cefotaxime, whereas vancomycin, linezolid, daptomycin, and tigecycline remained highly effective. Younger non-ICU patients (10–29) had higher MSSA, whereas older ICU ones showed pronounced HA-MRSA profiles. By the virtue of methicillin resistance, all MRSA were classified as multidrug resistance. Thus, MRSA colonization of the two extremes of life mostly in ICU seniors and the dominance of invasive MSSA and CA-MRSA patterns in non-ICU youth imply early age- and gender-specific adaptations of the three lineages. MRSA colonizes both ICU and non-ICU populations at extremes of age and gender specifically. High β-lactam resistance underscores the importance of robust stewardship and age- and gender-specific targeting in screening. These findings also indicate host- and organ-specificity in the sequalae of MSSA, CA-MRSA, and HA-MRSA evolutionary dynamics, emphasizing the need for continued surveillance to mitigate MRSA transmission and optimize patient outcomes in tertiary care settings.

Rosai-Dorfman Disease is a rare histiocytic disorder that is characterized by the accumulation of histiocytes in various tissues, most commonly the lymph nodes. Cutaneous RDD is a rare presentation, accounting for less than 10% of all RDD cases. We are reporting a cutaneous Rosai-Dorfman Disease clinically presented for the first time with a dome-like lesion over an indurated plaque in an 18-year-old female. Both lesions were biopsied, and findings suggested Rosai-Dorfman Disease. The patient was referred to an Oncologist with a subsequent PET scan showing hypermetabolic subcutaneous uptake on bilateral thighs and bilateral lumbar lesions, likely corresponding to the patient’s biopsy-proven histiocytosis in addition to a diffuse increased tracer uptake in the thoracic aorta and atria. A clinician should have a high degree of suspicion when two morphologies present in proximity to rule out a single pathological process. This case is unique because of the rarity of the condition as well as the clinical presentation of two different morphologies of an indurated plaque and a dome-like lesion on the same area of the body.

Background/Objectives : Non-communicable diseases like obesity, diabetes mellitus (DM), and hypertension (HTN) impose major global burdens. Helicobacter pylori infection may worsen these conditions. This study examined the clinical, age-, and gender-specific profiles of H. pylori-infected inpatients and its association with dyslipidemia, obesity, HTN, DM, and other chronic conditions. Methods: Between September 2024 and February 2025, patients were tested using a stool antigen assay (SIMA CHECK–ACON BIOTEC). Demographic data, smoking status, and clinical diagnoses (including kidney, liver, and heart disease) were obtained from hospital records and confirmed with standard criteria. Associations were analyzed using chi-square/Fisher’s tests and logistic regression (adjusted for age, gender, and smoking). Results: Overall, 30.7% (64/208) of inpatients were H. pylori–positive, highest in middle-aged females (42.9%) and lowest in middle-aged males (20.9%). Dyslipidemia rose sharply in males (5.6%→77.1%) versus females (5.7%→58.3%), with infection modestly elevating rates (OR=1.648). Obesity declined with age (males: 36.1%→22.9%; females: 45.7%→20.8%) yet strongly correlated with H. pylori (OR=19.217). HTN (5.6 – 94.3% in males; 11.4 – 100% in females) and DM (16.7 – 80% vs. 17.1 – 95.8%) increased with age but showed no infection link reflecting inflammatory preference. Smoking peaked at 61.1% in younger males. Kidney and heart diseases appeared only in ages 60–85. Overall, the prevalence of comorbidities markedly increased with age. Conclusion: H. pylori infection is common among inpatients, particularly in middle-aged females, and is significantly linked to dyslipidemia and obesity. These findings support targeted screening, especially in females and individuals with metabolic abnormalities, while future studies should assess whether eradication therapy can mitigate chronic metabolic risks.

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a devastating global health concern. Hypervirulent-strains are on the rise causing morbidities and mortalities worldwide. In tertiary care hospitals, critically-ill, invasive-procedures, pediatrics, and geriatrics are at risk. It is not clear how the strains specialized in humans in a clonal-genome. This study investigates the influence of age-, gender-, and source- (clinical, ICU vs. non-ICU)-specificity profiles in the evolution into hospital-associated (HA-MRSA) versus community-associated (CA-MRSA) lineages. Methods: 253 non-duplicate S. aureus isolates were obtained from May 2023 to March 2025). Patients were stratified by age and gender in ICU and non-ICUs. Standard microbiology methods and CLSI guidelines were used for identification and susceptibility testing, with cefoxitin and oxacillin disk diffusions and molecular diagnosis confirming MRSA. Mann-Whitney U and Chi-square tests assessed demographic distributions, clinical specimen sources, and MRSA/MSSA prevalence. Results: Of 253, 41.9% originated from ICUs (71% male, 29% female) and 58.1% from non-ICU wards (64% male, 36% female). In both settings, MRSA colonized the two extremes of age (10–29 and 70+) for males and females, with different mid-life peaks or declines by gender. However, the overall demographic distribution did not differ significantly between ICU and non-ICU groups (p = 0.287). Respiratory specimens constituted 37% and had the highest MRSA rate (42%), followed by blood (24.5%) and wounds (10.3%). In contrast, MSSA dominated wound (20.3%). Overall, 73.9% were resistant to cefoxitin and cefotaxime, whereas vancomycin, linezolid, daptomycin, and tigecycline remained highly effective. Younger non-ICU patients (10–29) had higher MSSA, whereas older ICU ones showed pronounced HA-MRSA profiles. By the virtue of methicillin-resistance, all MRSA were classified as multidrug resistance. Thus, MRSA colonization of the two extremes of life mostly in ICU seniors and the dominance of invasive MSSA and CA-MRSA patterns in non-ICU youth, imply early age- and gender-specific adaptations of the three lineages. Conclusion: MRSA colonizes both ICU and non-ICU populations at extremes of age gender-specificically. High β-lactam-resistance underscores the importance of robust stewardship and age- and gender-specific targeting in screening. These findings also indicate host- and organ-specificity in sequalae of MSSA, CA-MRSA, HA-MRSA evolutionary-dynamics emphasizing the need for continued surveillance to mitigate MRSA transmission and optimize patient outcomes in tertiary care settings.