Accurately identifying Natura 2000 habitat areas with the support of remote sensing techniques is becoming increasingly feasible. Various data types and methods are used for this purpose, and the fusion of data from various sensors and temporal periods (terms) within the phenological cycle allows natural habitats to be precisely identified. This research was aimed at selecting optimal datasets to classify three grassland Natura 2000 habitats (codes 6210, 6410 and 6510) in the Ostoja Nidziańska Natura 2000 site in Poland based on hyperspectral imagery and botanical on-ground reference data acquired in three terms during one vegetative period in 2017 (May, July and September), as well as a digital terrain model (DTM) obtained by airborne laser scanning (ALS). The classifications were carried out using a random forest (RF) algorithm on minimum noise fraction (MNF) transform output bands obtained for single terms, as well as data fusion combining the topographic indices (TOPO) calculated from the DTM, multitemporal hyperspectral data, or a combination of the two. The classification accuracy statistics were analysed in various combinations based on the datasets and their terms of acquisition. Topographic indices improved the classification accuracy of habitats 6210 and 6410, with the greatest impact noted in increased classification accuracy of xerothermic grasslands. The best terms for identifying specific habitats were autumn for 6510 and summer for 6210 and 6410, while the best results overall were obtained by combining data from all terms. The highest obtained values of the F1 coefficient were 84.5% for habitat 6210, 83.2% for habitat 6410, and 69.9% for habitat 6510. Comparing the data fusion results for habitats 6210 and 6410, greater accuracy was obtained by adding topographic indices to multitemporal hyperspectral data, while for habitat 6510, greater accuracy was obtained by fusing only multitemporal hyperspectral data.

Aerial hyperspectral and multispectral satellite data are the two most commonly used datasets to identify natural and semi-natural vegetation. However, there is no documented analysis based on data from several areas concerning the difference in the classification accuracy of non-forest Natura 2000 habitat with the use of aerial hyperspectral and satellite multispectral data. Also, there is no recommendation, on which habitat can be classified with sufficient accuracy using free multispectral images. This study aimed to analyse the difference in classification accuracy of Natura 2000 habitats representing: meadows, grasslands, heaths and mires between data with different spectral resolutions and the results utility for nature conservation compared to conventional maps. The analysis was conducted in five study areas in Poland. The classification was performed on multispectral Sentinel-2 (S2) and hyperspectral HySpex (HS) images using the Random Forest algorithm. Based on the results, it can be stated that the use of HS data resulted in higher classification accuracy, on average 0.14, than using S2 images, regardless of the area of the habitat. However, the difference in accuracy was not constant, varying by area and habitat characterisation. Greater differences in accuracy were observed for areas where habitats were characterised by high α-diversity or β-diversity. The HS and S2 data make it possible to create maps that provide a great deal of new knowledge about the distribution of Natura 2000 habitats, which is necessary for the management of protected areas. The obtained results indicate that by using S2 images it is possible to identify, at a satisfactory level, alluvial meadows and grassland. For heaths and mires, using HS data improved the results, but it is also possible to acquire general distribution of these classes, whereas HS images are obligatory for mapping salt, Molinia and lowland hay meadows.