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The disorganized polarization of tumor-associated macrophages (TAMs) exerts a critical effect on tumor progression. MicroRNAs (miRNAs) in extracellular vesicles (EVs) secreted from cancer cells may contribute to this process. However, the relationship between TAMs and EVs-miRNAs-mediated regulation in esophageal squamous cell carcinoma (ESCC) remains unclear. In the present study, immunoaffinity magnetic beads combined with antiepithelial cell adhesion molecules (EpCAM) were used to isolate and identify EVs-miR-21-5p from the plasma of ESCC patients. An in vitro coculture system was designed to evaluate the effect of esophageal cancer cells with miR-21-5p overexpression on macrophage polarization. We found that phorbol myristate acetate-induced THP-1 macrophages took up EVs-miR-21-5p from EC109 or EC9706 cells and were transformed into M2 macrophages. This, in turn, contributed to the excessive migration and invasion of esophageal cancer cells. The mechanism underlying these changes may involve activation of M2 macrophages by upregulated ESCC-derived EVs-miR-21-5p through the PTEN/AKT/STAT6 pathway. This may result in esophageal cancer cell epithelial-mesenchymal transition (EMT) via TGF-β/Smad2 signaling. Our results indicate positive feedback between M2 macrophage polarization and EMT of esophageal cancer cells in the tumor microenvironment via shuttling of miR-21-5p in tumor-derived EVs.
Jing Song; Peiyan Yang; Xiuwen Li; Xinyi Zhu; Mengxin Liu; Xuexin Duan; Ran Liu. Esophageal Cancer-Derived Extracellular Vesicle miR-21-5p Contributes to EMT of ESCC Cells by Disorganizing Macrophage Polarization. Cancers 2021, 13, 4122 .
AMA StyleJing Song, Peiyan Yang, Xiuwen Li, Xinyi Zhu, Mengxin Liu, Xuexin Duan, Ran Liu. Esophageal Cancer-Derived Extracellular Vesicle miR-21-5p Contributes to EMT of ESCC Cells by Disorganizing Macrophage Polarization. Cancers. 2021; 13 (16):4122.
Chicago/Turabian StyleJing Song; Peiyan Yang; Xiuwen Li; Xinyi Zhu; Mengxin Liu; Xuexin Duan; Ran Liu. 2021. "Esophageal Cancer-Derived Extracellular Vesicle miR-21-5p Contributes to EMT of ESCC Cells by Disorganizing Macrophage Polarization." Cancers 13, no. 16: 4122.
Copper (Cu) pollution in water and agricultural soil has always been a worldwide concern. This research aims to investigate the health effects of copper exposure on Caenorhabditis elegans (C. elegans) under the existing environmental quality standards (1 mg/L and 2 mg/L) via lifespan, reproduction, biological markers and transcriptome analysis. The results showed that copper of these two environmental standards shorten the lifespan of nematodes, reduced the brood size, reduced the frequency of pharyngeal pumps and prolonged defecation time as aging-related behaviors, and increased the levels of aging-related markers ROS, MDA and H2O2. There was a certain effect trend for the two exposure concentrations. Further, the possible molecular mechanism of copper-induced aging and reproductive effects on C. elegans was explored. Differential gene expression analysis was performed, and 2332 genes (567 up- and 1765 down-regulated genes) in the 1 mg/L group, 2449 DEGs (724 up- and 1725 down-regulated genes) in the 2 mg/L group in response to copper treatment. The top 20 regulated genes were vit (vit-1, vit-3, vit-4) genes, col genes (col-35, col-72, col-114, col-123, col-164, col-183, col-185), eea-1, him-18 and grl-20, which suggested that cuticle collagen synthesis and yolk expression were disrupted by copper. Analysis of KEGG pathway showed copper exposure widely affects longevity regulation pathways, thereby promoting aging. In summary, the sequencing results extensively and deeply reveal the health hazards of environmentally relevant doses of copper exposure to C. elegans, and behavioral testing verified that copper promoted aging of C. elegans.
Ying Zhang; Chao Zhao; Hu Zhang; Ran Liu; Shizhi Wang; Yuepu Pu; Lihong Yin. Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging. Ecotoxicology and Environmental Safety 2021, 222, 112494 .
AMA StyleYing Zhang, Chao Zhao, Hu Zhang, Ran Liu, Shizhi Wang, Yuepu Pu, Lihong Yin. Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging. Ecotoxicology and Environmental Safety. 2021; 222 ():112494.
Chicago/Turabian StyleYing Zhang; Chao Zhao; Hu Zhang; Ran Liu; Shizhi Wang; Yuepu Pu; Lihong Yin. 2021. "Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging." Ecotoxicology and Environmental Safety 222, no. : 112494.
Gut microbiota, especially human pathogens, has been shown to be involved in the occurrence and development of cancer. Esophageal squamous cell carcinoma and lung cancer are two malignant cancers, and their relationship with gut microbiota is still unclear. Virulence factor database (VFDB) is an integrated and comprehensive online resource for curating information about human pathogens. Here, based on VFDB database, we analyzed the differences of bacteria at genus level in the gut of patients with esophageal squamous cell carcinoma, lung cancer, and healthy controls. We proposed the possible cancer-associated bacteria in gut and put forward their possible effects. Apart from this, principal coordinate analysis (PCoA) and analysis of similarities (ANSOIM) suggested that some bacteria in the gut can be used as potential biomarkers to screen esophageal squamous cell carcinoma and lung cancer, and their effectiveness was preliminary verified. The relative abundance of Klebsiella and Streptococcus can be used to distinguish patients with esophageal squamous cell carcinoma and lung cancer from healthy controls. The absolute abundance of Klebsiella can further distinguish patients with esophageal squamous cell carcinoma from patients with lung cancer. In particular, the relative abundance of Fusobacterium can directly distinguish between patients with esophageal squamous cell carcinoma and healthy controls. Additionally, the absolute abundance of Haemophilus can distinguish lung cancer from healthy controls. Our study provided a new way based on VFDB database to explore the relationship between gut microbiota and cancer, and initially proposed a feasible cancer screening method.
Weitao Shen; Derong Tang; Yali Deng; Huilin Li; Tian Wang; Ping Wan; Ran Liu. Association of gut microbiomes with lung and esophageal cancer: a pilot study. World Journal of Microbiology and Biotechnology 2021, 37, 1 -16.
AMA StyleWeitao Shen, Derong Tang, Yali Deng, Huilin Li, Tian Wang, Ping Wan, Ran Liu. Association of gut microbiomes with lung and esophageal cancer: a pilot study. World Journal of Microbiology and Biotechnology. 2021; 37 (8):1-16.
Chicago/Turabian StyleWeitao Shen; Derong Tang; Yali Deng; Huilin Li; Tian Wang; Ping Wan; Ran Liu. 2021. "Association of gut microbiomes with lung and esophageal cancer: a pilot study." World Journal of Microbiology and Biotechnology 37, no. 8: 1-16.
The potential toxicity of copper has received great attention for a long time, however, trans-generational effects of copper have not been extensively investigated. Caenorhabditis elegans (C. elegans) was used to evaluate the trans-generational toxicities of copper several physiological endpoints: growth, head thrashes and body bends and degree of neuronal damage. Copper significantly inhibited growth, body bends, head thrashes and caused degeneration of dopaminergic neurons in a concentration-dependent manner in parental worms. Further we found oxidative damage was to underlying the onset of neuron degeneration. In our study copper promoted ROS accumulation, and led to an increased expression of the oxidative stress response-related genes sod-3 and a decreased expression of metal detoxification genes mtl-1 and mtl-2. Moreover, copper increased the fluorescence intensity of the transgenic strain that encodes the antioxidant enzyme SOD-3. Gradually decline in copper-induced impairments were observed in the filial generations without exposure. No growth impairment was shown in F3, the trend of head thrashes recovery gradually appeared in F2 and no growth impairment was shown in F3, the body bends impairment caused by the parental copper exposure was recovery until F4 and no growth impairment was shown in F5. Besides, dopamine neurons revealed damage related to neurobehavioral endpoints, with hereditary effects in the progeny together. In addition, sequencing results suggested that copper exposure could cause epigenetic changes. QRT-PCR results showed that differentially expressed genes can also be passed on to offspring.
Ying Zhang; Chao Zhao; Hu Zhang; Qiang Lu; Jingjing Zhou; Ran Liu; Shizhi Wang; Yuepu Pu; Lihong Yin. Trans-generational effects of copper on nerve damage in Caenorhabditis elegans. Chemosphere 2021, 284, 131324 .
AMA StyleYing Zhang, Chao Zhao, Hu Zhang, Qiang Lu, Jingjing Zhou, Ran Liu, Shizhi Wang, Yuepu Pu, Lihong Yin. Trans-generational effects of copper on nerve damage in Caenorhabditis elegans. Chemosphere. 2021; 284 ():131324.
Chicago/Turabian StyleYing Zhang; Chao Zhao; Hu Zhang; Qiang Lu; Jingjing Zhou; Ran Liu; Shizhi Wang; Yuepu Pu; Lihong Yin. 2021. "Trans-generational effects of copper on nerve damage in Caenorhabditis elegans." Chemosphere 284, no. : 131324.
Livestock manure and digestate (biogas slurry [BS] and biogas residue [BR]) contain bacteria that can potentially destroy the environment microecology and affect human health. This is a problem in China since it is a big producer of livestock and poultry. Thus, herein we have analyzed the bacterial content of both manure and digestate in 18 pig farms located in the Guangxi Zhuang Autonomous region. Specifically, we show that mid-temperature anaerobic fermentation affects both bacterial colony structure of swine manure (SM) and the relative abundance of bacteria and human pathogens. Indeed, the bacterial composition of SM changed after manure was fermented into BR and BS. These changes were dependent on bacterial adaptability; although some bacteria were reduced after fermentation, we observed an increase in Novosphingobium, Acidovorax, Longilinea, and Mycobacterium. The first three can potentially degrade polycyclic aromatic hydrocarbons and other harmful substances and may have a beneficial effect on the control of soil environmental pollution. However, the increased abundance of Mycobacterium poses a greater threat to human health. Thus, the transition from SM to digestate in the current condition for mid-temperature anaerobic fermentation needs to be optimized to reduce the entry of potential human pathogens in the soil after anaerobic fermentation.
Weitao Shen; Yue Yu; Rong Zhou; Ninghui Song; Ran Liu; Yuanqing Bu. Occurrence, Distribution, and Potential Role of Bacteria and Human Pathogens in Livestock Manure and Digestate: Insights from the Guangxi, China. Environmental Engineering Science 2021, 1 .
AMA StyleWeitao Shen, Yue Yu, Rong Zhou, Ninghui Song, Ran Liu, Yuanqing Bu. Occurrence, Distribution, and Potential Role of Bacteria and Human Pathogens in Livestock Manure and Digestate: Insights from the Guangxi, China. Environmental Engineering Science. 2021; ():1.
Chicago/Turabian StyleWeitao Shen; Yue Yu; Rong Zhou; Ninghui Song; Ran Liu; Yuanqing Bu. 2021. "Occurrence, Distribution, and Potential Role of Bacteria and Human Pathogens in Livestock Manure and Digestate: Insights from the Guangxi, China." Environmental Engineering Science , no. : 1.
Acute pulmonary embolism (APE) is a common sudden venous thromboembolism with high rates of morbidity and mortality. Several studies have concluded that microRNA-134 could be a potential biomarker for APE. However, the sensitivity of these studies varies widely. This study aimed to evaluate the diagnostic value of circulating microRNA-134 levels for APE. Four databases were searched to retrieve articles focusing on microRNA-134 detection in APE diagnosis. The Quality Assessment of Diagnostic Accuracy Studies-2 was used to evaluate the quality of the included literature. This meta-analysis included seven studies and 383 subjects. The microRNA-134 levels in APE patients were higher than those in controls (SMD = 2.84, z = 3.69, p < 0.001). The pooled sensitivity, specificity, and diagnostic odds ratio were 0.86 (0.72–0.94), 0.75 (0.66–0.82), and 19 (7–51), respectively. The positive and negative likelihood ratios were 3.4 (2.4–4.8) and 0.18 (0.08–0.40), respectively. The area under the summary receiver operating characteristic curve was 0.81 (0.77–0.84). Circulating microRNA-134 may be a new biomarker for the diagnosis of APE, but more tests and studies are needed to further explore and prove this. Trial registration number: PROSPERO registration #CRD42020184072
Yu Liu; Ming Xie; Xiaoli Gao; Ran Liu. Predictive Value of Circulating microRNA-134 Levels for Early Diagnosis of Acute Pulmonary Embolism: Meta-analysis. Journal of Cardiovascular Translational Research 2021, 14, 744 -753.
AMA StyleYu Liu, Ming Xie, Xiaoli Gao, Ran Liu. Predictive Value of Circulating microRNA-134 Levels for Early Diagnosis of Acute Pulmonary Embolism: Meta-analysis. Journal of Cardiovascular Translational Research. 2021; 14 (4):744-753.
Chicago/Turabian StyleYu Liu; Ming Xie; Xiaoli Gao; Ran Liu. 2021. "Predictive Value of Circulating microRNA-134 Levels for Early Diagnosis of Acute Pulmonary Embolism: Meta-analysis." Journal of Cardiovascular Translational Research 14, no. 4: 744-753.
A number of studies have identified that gut microbiota influences the development of cancer. However, there is little known about gut microbiota and esophageal cancer (EC). The aim of this study was to investigate the gut microbiota profile associated with EC. In this study, 23 patients with EC and 23 sex- and age-matched healthy controls (NC) were recruited between July 2019 and August 2019 at Huai'an First People's Hospital (Huai'an, China) and the gut microbiota was analyzed by 16S rRNA gene sequencing of fresh stool samples. We found that the microbial richness of intestinal flora in patients with EC were higher than NC, whereas evenness did not change obviously. Principal coordinate analysis (PCoA) and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) analysis both revealed that a distinct separation in bacterial community composition between the EC and NC. At the phylum level, the EC group showed significantly higher abundances of Firmicutes and Actinobacteria, but a lower Bacteroidetes than NC. At the genus level, a significantly increased abundance of Streptococcus, Bifidobacterium, Subdoligranulum, Blautia, Romboutsia, Collinsella, Paeniclostridium, Dorea, and Atopobium were observed in EC patients, while Lachnospira, Bacteroides, Agathobacter, Lachnoclostridium, Parabacteroides, Paraprevotella, Butyricicoccus, Tyzzerella, Fusicatenibacter, and Sutterella were reduced. Receiver operating characteristic (ROC) analysis revealed that Lachnospira, Bacteroides, Streptococcus, and Bifidobacterium both achieved a high accuracy in EC diagnosis (area under the curve was more than 0.85), and the Lachnospira was found to be the best classifier. This study firstly characterized the gut microbiota composition of EC patients and screened out the optimal potential microbiota biomarkers for EC diagnosis. It may provide a fundamental reference for further studies on the gut microbiome for the diagnosis and treatment of EC.
Yali Deng; Derong Tang; Panfei Hou; Weitao Shen; Huilin Li; Tian Wang; Ran Liu. Dysbiosis of gut microbiota in patients with esophageal cancer. Microbial Pathogenesis 2020, 150, 104709 .
AMA StyleYali Deng, Derong Tang, Panfei Hou, Weitao Shen, Huilin Li, Tian Wang, Ran Liu. Dysbiosis of gut microbiota in patients with esophageal cancer. Microbial Pathogenesis. 2020; 150 ():104709.
Chicago/Turabian StyleYali Deng; Derong Tang; Panfei Hou; Weitao Shen; Huilin Li; Tian Wang; Ran Liu. 2020. "Dysbiosis of gut microbiota in patients with esophageal cancer." Microbial Pathogenesis 150, no. : 104709.
Dysregulation of microRNAs (miRNAs) is induced during tumorigenesis. Our previous research suggested that HPV and MNNG led to malignant transformation of esophageal epithelial cells. To investigate the regulation and function of miR-218(miR-218-5p) during the malignant transformation of esophageal epithelial cells, we found miR-218 was inhibited synergistically by HPV and MNNG, suppressing cell proliferation, migration and invasion by up-regulating 3′ untranslated region (3′UTR) GAB2 in Het-1A-HPV-MNNG cells (malignant Het-1A cells induced by HPV and MNNG). A negative correlation was found between miR-218 and GAB2 mRNA expression in esophageal cancer patients and control people. GAB2 was up-regulated in Het-1A-HPV-MNNG cells. Further, down-expression of GAB2 reversed HPV&MNNG-mediated activation of migration and invasion and repressed SHP2/ERK and Akt/mTOR pathway signaling. In conclusion, miR-218 partially accounts for the prevention effect during malignant transformation of normal esophageal epithelial cells, which targets GAB2, which supplies the potential treatment in cancer therapy.
Ying Zhang; Yuhong Zheng; Enchun Pan; Chao Zhao; Hu Zhang; Ran Liu; Shizhi Wang; Yuepu Pu; Lihong Yin. Synergism of HPV and MNNG repress miR-218 promoting Het-1A cell malignant transformation by targeting GAB2. Toxicology 2020, 447, 152635 .
AMA StyleYing Zhang, Yuhong Zheng, Enchun Pan, Chao Zhao, Hu Zhang, Ran Liu, Shizhi Wang, Yuepu Pu, Lihong Yin. Synergism of HPV and MNNG repress miR-218 promoting Het-1A cell malignant transformation by targeting GAB2. Toxicology. 2020; 447 ():152635.
Chicago/Turabian StyleYing Zhang; Yuhong Zheng; Enchun Pan; Chao Zhao; Hu Zhang; Ran Liu; Shizhi Wang; Yuepu Pu; Lihong Yin. 2020. "Synergism of HPV and MNNG repress miR-218 promoting Het-1A cell malignant transformation by targeting GAB2." Toxicology 447, no. : 152635.
Polycystic ovary syndrome (PCOS) is reported to be associated with certain trace elements. However, previous data are inconsistent and potentially biased due to small sample sizes. The potential utility of trace element levels for screening of PCOS remains to be established. The aim of this meta-analysis was to investigate the potential relationships between PCOS and serum levels of zinc (Zn), copper (Cu), magnesium (Mg), iron (Fe) and ferritin. We carried out a literature search of PubMed, EMBASE, and Web of Science for relevant cross-sectional/case-control studies published prior to October 2019. Random-effect models were used to estimate the overall standard mean differences (SMDs) between PCOS and healthy control subjects. The screening value of potential microelement biomarkers for PCOS was assessed using the receiver operating characteristic (ROC) curve. Twenty-one studies featuring 2,173 women with PCOS and 1,897 healthy women were selected for analysis. Our results showed that Cu and ferritin levels were significantly higher in women with PCOS than healthy controls, with SMDs of 0.52 [95% confidence interval (CI): 0.38–0.67, I2 = 47.6%] and 1.05 (95% CI: 0.25–1.86, I2 = 97.0%), respectively. The serum ferritin concentration was distinguished as a potential biomarker for PCOS based on the high area under ROC curve value of 0.71 (95% CI: 0.57–0.86). Although we did not identify a statistical association between serum Zn concentration and PCOS overall, the concentration of Zn in PCOS women with insulin resistance (IR) was lower than that in healthy women (SMD = −0.89, 95% CI: −1.73 to −0.06). Furthermore, the concentrations of Mg (SMD = 0.31, 95% CI: −0.32–0.94, I2 = 95.4%) and Fe (SMD = −0.59, 95% CI: −1.29–0.12, I2 = 97.2%) were not statistically significant between the PCOS and control groups. We generated hypothetical pathways for associations among serum Cu, ferritin and PCOS. The serum concentrations of both Cu and ferritin were significantly higher in women with PCOS, and ferritin was identified as a potential early indicator for PCOS screening. Further studies are essential to determine the specific underlying mechanisms.
Jiechen Yin; Xiang Hong; Jun Ma; Yuanqing Bu; Ran Liu. Serum Trace Elements in Patients With Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis. Frontiers in Endocrinology 2020, 11, 1 .
AMA StyleJiechen Yin, Xiang Hong, Jun Ma, Yuanqing Bu, Ran Liu. Serum Trace Elements in Patients With Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis. Frontiers in Endocrinology. 2020; 11 ():1.
Chicago/Turabian StyleJiechen Yin; Xiang Hong; Jun Ma; Yuanqing Bu; Ran Liu. 2020. "Serum Trace Elements in Patients With Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis." Frontiers in Endocrinology 11, no. : 1.
The widespread use of the herbicides Atrazine (ATR) has been raised attention due to its ubiquitous occurrence in the environment. As an endocrine disruptor, ATR causes reproductive, immune, nervous system toxicity in biota. In this study, we aimed to investigate metabolic profile characteristics and potential metabolic biomarker that reflects specific damage in toxic effect after ATR exposure. Hence, a metabolomics study was performed to determine the significantly affected metabolites and the reproduction and locomotion of C. elegans were investigated. Mediation analysis was used to evaluate the mediating effect of metabolites on association between ATR exposure and toxic effect. ATR (≥0.04 mg/L) caused the significant dose dependent reduction of brood size and locomotion behavior, however, the body length and width were significantly decreased only in 40 mg/L group. These results suggesting that brood size, head thrashes and body bends are more sensitive indictor to assessment ATR toxicity in C. elegans. Meanwhile, metabolomics analysis revealed that ATR exposure can induce metabolic profiles significant alterations in C. elegans. We found that 9 metabolites significantly increased and 18 metabolites significantly decreased, such as phosphatidylcholine, GMP, CDP-choline, neopterin etc. Those alteration of metabolites were mainly involved in the pathways: glycerophospholipid metabolism, glycolysis/gluconeogenesis, folate biosynthesis, glycine, serine and threoninemetabolism, pyrimidine and purine metabolism. Overall, these changes are signs of possible oxidative stress and ATP synthesis disruption modification. Mediation analysis showed a significant indirect effect of ATR exposure on brood size, via 7,8-dihydroneopterin 2′,3′-cyclic-p, and phosphatidylcholine might mediate association between ATR exposure and body bends, suggesting that 7,8-dihydroneopterin 2′,3′-cyclic-p and phosphatidylcholine might be potentially specificity marker for brood size and body bend respectively. This preliminary analysis investigates metabolic characteristics in C. elegans after ATR exposure, helping to understand the pathways involved in the response to ATR exposure and provide potential biomarkers for the safety evaluation of ATR.
Jiechen Yin; Xiang Hong; Lingyi Ma; Ran Liu; Yuanqing Bu. Non-targeted metabolomic profiling of atrazine in Caenorhabditis elegans using UHPLC-QE Orbitrap/MS. Ecotoxicology and Environmental Safety 2020, 206, 111170 .
AMA StyleJiechen Yin, Xiang Hong, Lingyi Ma, Ran Liu, Yuanqing Bu. Non-targeted metabolomic profiling of atrazine in Caenorhabditis elegans using UHPLC-QE Orbitrap/MS. Ecotoxicology and Environmental Safety. 2020; 206 ():111170.
Chicago/Turabian StyleJiechen Yin; Xiang Hong; Lingyi Ma; Ran Liu; Yuanqing Bu. 2020. "Non-targeted metabolomic profiling of atrazine in Caenorhabditis elegans using UHPLC-QE Orbitrap/MS." Ecotoxicology and Environmental Safety 206, no. : 111170.
As a persistent organic pollutant, perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) have gained increasing research attention over recent years because of their potential risk to humans and the environment. In this paper, we investigated the reproductive toxicity of these pollutants using a C. elegans model to evaluate spermatogenesis throughout the entire developmental cycle of him-5 mutant by exposing to 0.001, 0.01, and 0.1 mmol/L PFOS or PFOA for 48 h. Experimental results suggested that PFOS and PFOA exposure led to reductions in brood size, germ cell number, spermatid size, and motility, and increases in rate of malformation spermatids. Analysis of variance (ANOVA) showed that exposure to PFOS resulted in higher levels of damage than PFOA in germ cells only in 0.001 mmol/L exposure group. RT-qPCR was used to further investigate the expression of genes associated with different stages of spermatogenesis, such as mitosis and meiosis, fibrous body-membranous organelles (FB-MOs), and sperm activation. The expression levels of wee-1.3, spe-4, spe-6, and spe-17 genes were increased, while those of puf-8, spe-10, fer-1, swm-1, try-5, and spe-15 genes were decreased. Our results suggesting that PFOS or PFOA may cause spermatogenesis damage by disrupting the mitotic proliferation, meiotic entry, formation of the MOs, fusion of the MOs and plasma membrane (PM), and pseudopods. Loss-of-function studies using puf-8 and spe-10 mutants revealed spe-10 gene was specifically involved in PFOS- or PFOA-induced reproductive toxicity via regulating one or more critical palmitoylation events, while puf-8 gene was not direct target of PFOS and PFOA, and PFOS and PFOA may act on the upstream gene of puf-8, thus affecting reproductive ability. Taken together, these results demonstrate the potential adverse impact of PFOS and PFOA exposure on spermatogenesis and provide valuable data for PFC risk assessment. Grapical abstract
Jiechen Yin; Zihai Jian; Guangcan Zhu; Xiaojin Yu; Yuepu Pu; Lihong Yin; Dayong Wang; Yuanqing Bu; Ran Liu. Male reproductive toxicity involved in spermatogenesis induced by perfluorooctane sulfonate and perfluorooctanoic acid in Caenorhabditis elegans. Environmental Science and Pollution Research 2020, 28, 1443 -1453.
AMA StyleJiechen Yin, Zihai Jian, Guangcan Zhu, Xiaojin Yu, Yuepu Pu, Lihong Yin, Dayong Wang, Yuanqing Bu, Ran Liu. Male reproductive toxicity involved in spermatogenesis induced by perfluorooctane sulfonate and perfluorooctanoic acid in Caenorhabditis elegans. Environmental Science and Pollution Research. 2020; 28 (2):1443-1453.
Chicago/Turabian StyleJiechen Yin; Zihai Jian; Guangcan Zhu; Xiaojin Yu; Yuepu Pu; Lihong Yin; Dayong Wang; Yuanqing Bu; Ran Liu. 2020. "Male reproductive toxicity involved in spermatogenesis induced by perfluorooctane sulfonate and perfluorooctanoic acid in Caenorhabditis elegans." Environmental Science and Pollution Research 28, no. 2: 1443-1453.
Concerns have been raised over the safety and health of industrial workers exposed to indium oxide nanoparticles (IO‐NPs) when working. IO‐NPs were previously shown in vitro and in vivo to be cytotoxic, but the mechanism of pathogenesis was unclear. In this study, the effects of IO‐NPs on lung cells associated with respiratory and immune barriers and the toxic effects of intercellular cascades were studied. Here IO‐NPs had acute toxicity to Wistar rats over a time course (5 days post‐intratracheal instillation). Following treatment epithelial cells (16HBE) or macrophages (RAW264.7) with IO‐NPs or IO fine particles (IO‐FPs), the damage of 16HBE cells caused by IO‐NPs was serious, mainly in the mitochondrial and rough endoplasmic reticulum. The lactate dehydrogenase level also showed that cytotoxicity in vitro was more serious for IO‐NPs compared with IO‐FPs. The level of In3+ (examined by inductively coupled plasma mass spectrometry) in 16HBE cells was 10 times higher than that in RAW cells. In3+, releasing from IO‐NPs absorbed by 16HBE cells, could not only significantly inhibit the phagocytosis and migration of macrophages (P < .0001), but also stimulate RAW cells to secrete high levels of inflammatory cytokines. IO‐NPs can directly damage pulmonary epithelial cells. The In3+ released by epithelial cells affect the phagocytosis and migration of macrophages, which may be a new point for the decrease in the clearance of alveolar surfactants and the development of IO‐related pulmonary alveolar proteinosis.
Huilin Li; Zhaofang Chen; Jinxia Li; Ru Liu; Feng Zhao; Ran Liu. Indium oxide nanoparticles induce lung intercellular toxicity between bronchial epithelial cells and macrophages. Journal of Applied Toxicology 2020, 40, 1636 -1646.
AMA StyleHuilin Li, Zhaofang Chen, Jinxia Li, Ru Liu, Feng Zhao, Ran Liu. Indium oxide nanoparticles induce lung intercellular toxicity between bronchial epithelial cells and macrophages. Journal of Applied Toxicology. 2020; 40 (12):1636-1646.
Chicago/Turabian StyleHuilin Li; Zhaofang Chen; Jinxia Li; Ru Liu; Feng Zhao; Ran Liu. 2020. "Indium oxide nanoparticles induce lung intercellular toxicity between bronchial epithelial cells and macrophages." Journal of Applied Toxicology 40, no. 12: 1636-1646.
Background Angiogenesis, a pivotal component in the tumor microenvironment (TME), boosts tumor growth and metastasis. Cancer-derived exosomes, which have been widely reported to play a crucial role in the establishment of TME can be effective angiogenic modulators. We aim to investigate the contribution of microRNA-21 (miR-21) for angiogenesis which was packaged in cancer-derived exosomes in esophageal squamous cell carcinoma (ESCC). Methods The co-cultivation model was constructed to mimic the tumor microenvironment based at a physical level to explore the effects of cancer-derived exosomes on angiogenesis of human umbilical vein endothelial cells (HUVECs). EdU assay, transwell assay and tube formation assay formation experiments were conducted for the evaluation of HUVECs proliferation, migration, and angiogenesis, respectively. In addition, Dual-luciferase reporter (DLR) assay was performed to validate the relationship between miR-21 and its target gene PTEN. Similarly, miR-21 inhibitors and LY294002 was applied to evaluate the regulation of miR-21 via pro-angiogenesis in recipient HUVECs by PTEN/Akt signaling pathway. Results After 24 h co-cultivation with EC9706 cells, miR-21 levels in recipient HUVECs was raised. The results from EdU assay, transwell assay and blood vessel formation experiment showed that exosomes which were secreted from EC9706 cells (EC9706-Exo) delivered miR-21 stimulated proliferation, migration and tube formation of HUVECs. DLR assay indicated that miR-21 could directly bind to the 3'-untranslated region (UTR) of PTEN genes, real-time PCR and western blot analysis for PTEN showed it was inhibited by EC9706-Exo shuttled miR-21. Meanwhile, phospho-Akt (p-Akt) (Ser473), one of the downstream genes of PTEN, was significantly increased in recipient HUVECs compared to the control group, while inhibiting miR-21 and PI3K/Akt pathway respectively both led to a sharp decrease in p-Akt levels, suggesting that exosomal miR-21 promote angiogenesis via activating PTEN/Akt signaling pathway. Conclusion Exosomal miR-21 acts as a driver of pro-angiogenesis by activating PTEN/Akt signaling pathway, it might serve as a blood-based biomarker for ESCC metastasis. Suppressing the expression or blocking the transmission of these exosome-derived miR-21 might be a novel antiangiogenic therapeutic strategy for ESCC.
Tian Wang; Juan Liao; Zijie Yang; Weitao Shen; Zhi Kui Gao; Jie Chen Yin; Li Hong Yin; Ran Liu. Exosome-mediated miR-21 promotes angiogenesis within esophageal tumor microenvironment by activating PTEN/Akt signaling pathway in Vascular Endothelial Cells. 2020, 1 .
AMA StyleTian Wang, Juan Liao, Zijie Yang, Weitao Shen, Zhi Kui Gao, Jie Chen Yin, Li Hong Yin, Ran Liu. Exosome-mediated miR-21 promotes angiogenesis within esophageal tumor microenvironment by activating PTEN/Akt signaling pathway in Vascular Endothelial Cells. . 2020; ():1.
Chicago/Turabian StyleTian Wang; Juan Liao; Zijie Yang; Weitao Shen; Zhi Kui Gao; Jie Chen Yin; Li Hong Yin; Ran Liu. 2020. "Exosome-mediated miR-21 promotes angiogenesis within esophageal tumor microenvironment by activating PTEN/Akt signaling pathway in Vascular Endothelial Cells." , no. : 1.
The transgenerational reproductive and developmental toxicity of tebuconazole (TEB) in Caenorhabditis elegans was investigated over five generations (P0 - F4). Only parental C.elegans (P0) were exposed to TEB (0, 0.01, 0.1, 1, and 10 μg/L) for 24 h and the subsequent offspring (F1-F4) were grown under TEB-free conditions. TEB exposure caused dose-dependent reproductive defects and developmental impairments in C.elegans. In the P0 generation reproductive defects were observed such as: reduced brood size and embryo hatchability, prolonged generation time, retarded gonadal development, and slower germline proliferation, even at 0.01 μg/L, together with developmental toxicity with significant reduced body length and narrowed body width at 10 μg/L. Additionally, the brood size significantly reduced in F2, which began to recover from F3, but was still lower than the control in F4. The proportion of abnormalities increased significantly in F2 and reduced from F3, but was still higher than the control, suggesting that TEB could have cumulative potential and be passed to offspring through parental exposure. Furthermore, exposure to TEB (10 μg/L) in P0 significantly reduced the body length in F1, which began to recover from F2, and was the same level as the control in F4. There was a concentration-dependent increase in body width in F1-F4, with a significant increase only observed in F1 at 10 μg/L. Thus, parental exposure to TEB induced transgenerational defects in both reproduction and development, emphasizing the significance of considering bio-toxicity over multiple generations to conduct accurate assessment of environmental risks of toxicants.
Qian Lu; Yuanqing Bu; Lingyi Ma; Ran Liu. Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans. Journal of Applied Toxicology 2020, 40, 578 -591.
AMA StyleQian Lu, Yuanqing Bu, Lingyi Ma, Ran Liu. Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans. Journal of Applied Toxicology. 2020; 40 (5):578-591.
Chicago/Turabian StyleQian Lu; Yuanqing Bu; Lingyi Ma; Ran Liu. 2020. "Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans." Journal of Applied Toxicology 40, no. 5: 578-591.
The long intergenic noncoding RNA, regulator of reprogramming (linc‐ROR) has been reported to participate in tumorigenesis, while its functions and fundamental mechanisms in esophageal squamous cell carcinoma (ESCC) remain unclear. In this study, gain‐of‐function assays showed that linc‐ROR upregulation enhanced cell viability, promoted cell proliferation, and inhibited apoptosis. Mechanistically, the regulatory network of linc‐ROR/miR‐204‐5p/MDM2 was established with bioinformatics analysis and online databases, then validated via dual‐luciferase reporter assays, RNA immunoprecipitation assays in ESCC cells. Linc‐ROR positively regulates the expression of MDM2 as a molecular sponge of miR‐204‐5p. Moreover, results of western blot and coimmunoprecipitation indicated that linc‐ROR overexpression enhanced the ubiquitination level of p53, and its downstream apoptosis‐related genes have showed higher bcl‐2 expression, lower bax, and cleaved caspase‐3 expressions, while miR‐204‐5p could counteract with this effect. Finally, small interfering RNAs tailored to linc‐ROR were established to further evaluate its effects on ESCC comprehensively. In summary, this study revealed that linc‐ROR modulated cell apoptosis and regulated p53 ubiquitination via targeting miR‐204‐5p/MDM2 axis, which provides a novel therapeutic insight into treatments for ESCC.
Han Gao; Tian Wang; Peng Zhang; Muhe Shang; Zhikui Gao; Fei Yang; Ran Liu. Linc‐ROR regulates apoptosis in esophageal squamous cell carcinoma via modulation of p53 ubiquitination by targeting miR‐204‐5p/MDM2. Journal of Cellular Physiology 2019, 235, 2325 -2335.
AMA StyleHan Gao, Tian Wang, Peng Zhang, Muhe Shang, Zhikui Gao, Fei Yang, Ran Liu. Linc‐ROR regulates apoptosis in esophageal squamous cell carcinoma via modulation of p53 ubiquitination by targeting miR‐204‐5p/MDM2. Journal of Cellular Physiology. 2019; 235 (3):2325-2335.
Chicago/Turabian StyleHan Gao; Tian Wang; Peng Zhang; Muhe Shang; Zhikui Gao; Fei Yang; Ran Liu. 2019. "Linc‐ROR regulates apoptosis in esophageal squamous cell carcinoma via modulation of p53 ubiquitination by targeting miR‐204‐5p/MDM2." Journal of Cellular Physiology 235, no. 3: 2325-2335.
Cancer stem cells (CSCs) are closely related to tumor resistance and tumor recurrence in esophageal squamous cell carcinoma (ESCC). The lack of specific biomarkers to identify and isolate CSCs has led to the slow progression of research on CSCs in ESCC. Here, we established a method to identify and isolate CSCs in ESCC using fluorescence-activated cell sorting with combined surface biomarkers including CD71, CD271, and CD338. CD71−/CD271+/CD338+ subpopulation cells possessed more stem cell properties in proliferation, self-renewal, differentiation, metastasis, drug resistance, and tumorigenesis. We further explored possible roles that microRNAs played in stem cells. Using microarrays, we identified that has-miR-21-3p was highly expressed in positive sorted cells, and further functional and Luciferase reporter assays verified that has-miR-21-3p promoted proliferation and anti-apoptosis by regulating TRAF4. We further analyzed the relationship between hsa-miR-21-3p and ESCC in 137 patients with ESCC. Statistical analysis showed that up-regulation of hsa-miR-21-3p was associated with a high risk of ESCC. Collectively, we identified surface biomarkers of stem cells in esophageal squamous cell carcinoma, and discovered thathsa-miR-21-3p may be involved in stemness maintenance by regulating TRAF4.
Zhikui Gao; Hui Liu; Yajuan Shi; Lihong Yin; Yong Zhu; Ran Liu. Identification of Cancer Stem Cell Molecular Markers and Effects of hsa-miR-21-3p on Stemness in Esophageal Squamous Cell Carcinoma. Cancers 2019, 11, 518 .
AMA StyleZhikui Gao, Hui Liu, Yajuan Shi, Lihong Yin, Yong Zhu, Ran Liu. Identification of Cancer Stem Cell Molecular Markers and Effects of hsa-miR-21-3p on Stemness in Esophageal Squamous Cell Carcinoma. Cancers. 2019; 11 (4):518.
Chicago/Turabian StyleZhikui Gao; Hui Liu; Yajuan Shi; Lihong Yin; Yong Zhu; Ran Liu. 2019. "Identification of Cancer Stem Cell Molecular Markers and Effects of hsa-miR-21-3p on Stemness in Esophageal Squamous Cell Carcinoma." Cancers 11, no. 4: 518.
Microcystin-LR is a cyclic heptapeptide hepatotoxin produced by harmful cyanobacteria. A panel of microRNAs containing miR-451a were found to be significantly changed in normal human liver cells HL7702 after exposure to microcystin-LR (MC-LR) in our previous study. However, the functions of miR-451a in hepatotoxicity induced by MC-LR remained unclear. The study aimed to investigate the impacts of miR-451a in HL7702 cells following treatment with 5 or 10 μM MC-LR. The comet assay indicated that MC-LR can influence Olive tail moment (OTM) in HL7702 cells. Furthermore, increase of miR-451a significantly repressed DNA damage and the protein expression level of γ-H2AX induced by MC-LR. Moreover, over-expression of miR-451a inhibited the expression level of p-AKT1 protein in cells following treatment by MC-LR. These results showed that miR-451a may protect from MC-LR-induced DNA damage by down-regulating the expression of p-AKT1, which provides new clues for the diagnosis and therapy policies for liver damage induced by MC-LR.
Lv Chen; Shu Yang; Cong Wen; Shuilin Zheng; Yue Yang; Xiangling Feng; Jihua Chen; Dan Luo; Ran Liu; Fei Yang. Regulation of Microcystin-LR-Induced DNA Damage by miR-451a in HL7702 Cells. Toxins 2019, 11, 164 .
AMA StyleLv Chen, Shu Yang, Cong Wen, Shuilin Zheng, Yue Yang, Xiangling Feng, Jihua Chen, Dan Luo, Ran Liu, Fei Yang. Regulation of Microcystin-LR-Induced DNA Damage by miR-451a in HL7702 Cells. Toxins. 2019; 11 (3):164.
Chicago/Turabian StyleLv Chen; Shu Yang; Cong Wen; Shuilin Zheng; Yue Yang; Xiangling Feng; Jihua Chen; Dan Luo; Ran Liu; Fei Yang. 2019. "Regulation of Microcystin-LR-Induced DNA Damage by miR-451a in HL7702 Cells." Toxins 11, no. 3: 164.
Esophageal cancer is one of the most common cancers worldwide. It is critical to find early diagnostic biomarkers for esophageal cancer. MicroRNAs (miRNAs) play important regulatory roles in occurrence and development of esophageal cancer, which has the diagnostic and prognostic values. The aim of the study was to evaluate the potential diagnostic value of the novel miRNA. The novel miRNA was predicted using miRDeep2 software and validated by quantitative reverse transcription PCR (qRT-PCR) and the TA-cloning sequencing of the PCR products. The expression of the novel miRNA in esophageal cancer tissues and adjacent tissues was also analyzed by qRT-PCR. The EdU staining and transwell method were used to detect the capacity of cell proliferation, migration, and invasion. Besides, the target gene CTNNA2 of novel-miR-4885 was verified via qRT-PCR, western blot, luciferase reporter assay, and RNA immunoprecipitation. We identified the novel-miR-4885, the expression level was confirmed by qRT-PCR in the esophageal cancer cells. The result of TA-cloning sequencing was consistent with the prediction and the pre-miRNA had a standard hairpin stem-loop structure. In addition, the expression of novel-miR-4885 was upregulated in esophageal cancer tissue compared with that in adjacent tissue (p < 0.05). Further, the assays showed that overexpression novel-miR-4885 could improve the cell proliferation, migration, and invasion with an average fold change of 1.19, 1.59, and 2.34, respectively. Novel-miR-4885 can bind to 3′ untranslated region of CTNNA2 to reduce cell adhesion and promote epithelial–mesenchymal transition in esophageal cancer cell. The expression of N-cadherin, β-catenin, Vimentin, and α-smooth muscle actin was upregulated, while that of E-cadherin and ZO-1 proteins was downregulated through western blot. Novel miRNA present in esophageal cancer cells was validated, supplementing the miRNA database. Meantime, the possible functional mechanisms were explored, and the results showed that the novel miRNA may serve as potential biomarker for the diagnosis of esophageal cancer.
Jing Song; Peng Zhang; Mengxin Liu; Ming Xie; Zhikui Gao; Xianghu Wang; Tian Wang; Jiechen Yin; Ran Liu. Novel-miR-4885 Promotes Migration and Invasion of Esophageal Cancer Cells Through TargetingCTNNA2. DNA and Cell Biology 2019, 38, 151 -161.
AMA StyleJing Song, Peng Zhang, Mengxin Liu, Ming Xie, Zhikui Gao, Xianghu Wang, Tian Wang, Jiechen Yin, Ran Liu. Novel-miR-4885 Promotes Migration and Invasion of Esophageal Cancer Cells Through TargetingCTNNA2. DNA and Cell Biology. 2019; 38 (2):151-161.
Chicago/Turabian StyleJing Song; Peng Zhang; Mengxin Liu; Ming Xie; Zhikui Gao; Xianghu Wang; Tian Wang; Jiechen Yin; Ran Liu. 2019. "Novel-miR-4885 Promotes Migration and Invasion of Esophageal Cancer Cells Through TargetingCTNNA2." DNA and Cell Biology 38, no. 2: 151-161.
High ambient temperature in poultry is a challenging and fatal stress among environmental factors. It affects the production quality, damages the liver, and increases mortality in broilers. The present study is focused to explore appropriate utilization of Selenium (Se) as a feed additive in broiler chickens against high temperature. Day-old male broiler chickens (Ross 308) (n = 200) were grouped according to the supplements used in their basal diets such as: corn-soybean basal diet as control (Con), a basal diet containing sodium selenite, basal diet with probiotics, and a basal diet containing selenium-enriched probiotics (SP). At the end of the experimental period of 42 days, the liver was isolated and was used to determine the antioxidant capacity through a spectrophotometer. Inflammatory and anti-inflammatory cytokines production in the liver was measured through a real-time polymerase chain reaction. Hepatic analyses revealed the decreased level of malondialdehyde, whereas glutathione, glutathione peroxidase (GSH-Px), and superoxide dismutase levels were increased in the SP group. Furthermore, supplementation of SP significantly up-regulated the mRNA expression of glutathione peroxidase 1 (GPx1), GPx4, IL6, and IL10 and down-regulated the expression of pro-inflammatory cytokines. It is thus concluded that SP as a potential nutritive supplement may facilitate hepatic protection by suppressing hepatic oxidation, inflammation, and necrosis during the high ambient temperature of summer.
Alam Zeb Khan; Imdad Ullah Khan; Shakirullah Khan; Samreen Afzal; Mohammad Hamid; Muhammad Tariq; Ikram Ul Haq; Naimat Ullah; Mumtaz Ali Khan; Shahid Bilal; Kehe Huwang; Ran Liu. Selenium-enriched probiotics improve hepatic protection by regulating pro-inflammatory cytokines and antioxidant capacity in broilers under heat stress conditions. Journal of Advanced Veterinary and Animal Research 2019, 6, 355 -361.
AMA StyleAlam Zeb Khan, Imdad Ullah Khan, Shakirullah Khan, Samreen Afzal, Mohammad Hamid, Muhammad Tariq, Ikram Ul Haq, Naimat Ullah, Mumtaz Ali Khan, Shahid Bilal, Kehe Huwang, Ran Liu. Selenium-enriched probiotics improve hepatic protection by regulating pro-inflammatory cytokines and antioxidant capacity in broilers under heat stress conditions. Journal of Advanced Veterinary and Animal Research. 2019; 6 (3):355-361.
Chicago/Turabian StyleAlam Zeb Khan; Imdad Ullah Khan; Shakirullah Khan; Samreen Afzal; Mohammad Hamid; Muhammad Tariq; Ikram Ul Haq; Naimat Ullah; Mumtaz Ali Khan; Shahid Bilal; Kehe Huwang; Ran Liu. 2019. "Selenium-enriched probiotics improve hepatic protection by regulating pro-inflammatory cytokines and antioxidant capacity in broilers under heat stress conditions." Journal of Advanced Veterinary and Animal Research 6, no. 3: 355-361.
MiRNA clusters are widely expressed across species, accumulating evidence has illustrated that miRNA cluster functioned more efficiently than single miRNA in cancer oncogenesis. It is likely that miRNA clusters are more stable and reliable than individual miRNA to be biomarkers for diagnosis and therapy. We previously found low expression of miR-144/451 was closely related with the risk for esophageal cancer. Researches on miR-144/451 cluster were mostly focused on individual miRNA but not the whole cluster, the regulatory mechanism of miRNA cluster were largely unknown. In present study, we firstly analysed biological functions of individual miRNAs of miR-144/451 in ECa9706 transfected with miRNA mimics. We further analysed the biological function of the whole cluster in stable transgenic cell overexpressing miR-144/451. We then performed genome-wide mRNA microarray to detect differentially expressed gene profiles in stable transgenic cells. Overexpression of miR-144-3p promoted early apoptosis of ECa9706 and inhibited cell migration, cell invasion and cell proliferation. miR-144-5p and miR-451a inhibited cell proliferation, at the same time, miR-451a inhibited cell migration. Overexpression of miR-144/451 leads to the arrest cell cycle from S to G2 and G2 to M,while the invasion ability was obviously inhibited. We further observed c-Myc, p-ERK were downregulated in cells overexpressing miR-144/451, while p53 was up-regulated. The downstream effectors of c-Myc, MMP9 and p-cdc2 were downregulated in miR-144/451 stable transgenic cell. miR-144/451 may or partly inhibited cell cycles and invasion of ECa9706 through inhibiting ERK/c-Myc signaling pathway. Collectively, we analysed the function of miR-144/451 cluster from individual to overall level. miR-144/451 cluster played proto oncogene role in esophageal cancer by inhibiting cell invasion.
Zhikui Gao; Peng Zhang; Ming Xie; Han Gao; Lihong Yin; Ran Liu. miR-144/451 cluster plays an oncogenic role in esophageal cancer by inhibiting cell invasion. Cancer Cell International 2018, 18, 184 .
AMA StyleZhikui Gao, Peng Zhang, Ming Xie, Han Gao, Lihong Yin, Ran Liu. miR-144/451 cluster plays an oncogenic role in esophageal cancer by inhibiting cell invasion. Cancer Cell International. 2018; 18 (1):184.
Chicago/Turabian StyleZhikui Gao; Peng Zhang; Ming Xie; Han Gao; Lihong Yin; Ran Liu. 2018. "miR-144/451 cluster plays an oncogenic role in esophageal cancer by inhibiting cell invasion." Cancer Cell International 18, no. 1: 184.