While potentially toxic elements (PTE) in lake have been widely studied, their enrichment, fate and health risks associated with lacustrine groundwater discharge (LGD) remain poorly understood. Thus, self-organizing maps (SOM) and positive matrix factorization (PMF) were employed in present work to decipher source of selected PTE (Li, V, Mn, Cr, Co, Ni, Cu, Zn, Ba, Pb, U, Sr) in Ulansuhai Lake basin (Inner Mongolia, China). On this basis, multi-isotopic tracers (δD/δ¹⁸O_water, ⁸⁷Sr/⁸⁶Sr, δ³⁴S/δ¹⁸O_sulfate, ²²²Rn) were used to indicate the geochemical processes related to PTE enrichment and fate. Groundwater exhibited higher PTE concentrations than surface waters, with Mn levels 230-fold higher (0.575 vs. 0.003 mg/L), while Cr, Ni, Zn, and Ba showed 2-, 2.2-, 3-, and 1.5-fold increases, respectively. 68 samples were grouped to four clusters (C1-C4) and characterized by a hazard gradient of C4 > C3 > C1 > C2. Quantitative source apportionment identified evaporite dissolution (46.2 %) as the dominant PTE contributor, followed by industrial (23.1 %) and redox processes (23.9 %), with minor agricultural inputs (6.8 %). LGD-mediated fluxes, particularly Mn (1.71 ± 0.05 mg/m²/d) and Sr (8.63 ± 0.23 mg/m²/d), substantially influenced lake water quality. Microbial analysis revealed higher diversity (ace index = 1751 ± 577) in groundwater, while surface water PTE had a more pronounced impact on microbial functionality than groundwater (p < 0.001). Health risk assessment demonstrated elevated hazards in PTE contaminated groundwater, posing higher non-carcinogenic (non-carcinogenic risk-HI: 1.43 vs 1.25) and carcinogenic risks (carcinogenic risk assessment-CR: 0.005 vs 0.003) than surface water, with children being more vulnerable (5, 8 and 7 cases per 1000 children were found at carcinogenic risk). This multi-method investigation elucidates LGD-driven PTE cycling fluxes and mechanisms, providing critical insights for groundwater-lake management.

China is one of the countries most seriously affected by soil salinization, while the impact of salt-controlled irrigation on the relationship between soil dissolved organic matter (DOM) and microbial in farmland affected by salinization remains largely unexplored. We conducted a comprehensive survey of soil DOM and a microbial survey of Ordos’s salinized farmland in China before and after salt-controlled irrigation. Our findings reveal a reduction of 18.4 mg/L in surface soil (0–10 cm) DOC following irrigation, whereas the subsurface soil (20–40 cm) DOC increased by 20.7 mg/L. Moreover, irrigation led to an increase in the aromaticity and humification of the soil, with the salt content of the subsurface soil rising from 2.7 to 3.7 mg/g. Additionally, the total dissolved solids (TDS) in the drained water were 2463 mg/L higher than in the irrigation water (1416.3 mg/L). This suggests that the DOM and salts from the surface soil either leached into deeper layers or were lost via runoff. Furthermore, SEM analysis and a Mantel test revealed that microbial composition significantly influenced soil DOM contents, especially increased levels of Marmoricola and MND1, which are associated with decomposing organic matter and may contribute to the leaching of soil DOM in deep layers following irrigation.