The effects of GroBiotic®-A supplementation on growth performance, body composition, liver and intestine histology in European seabass (Dicentrarchus labrax) juveniles were evaluated. The commercial GroBiotic®-A was added to diets at four different levels (0, 1, 2 and 3%), three replicates and fed 4 times a day (9:00, 11:30, 14:00, 16: 30 hours) for 60 days as ad libitum. Total 480 European seabass juveniles with a starting weight of 1.40±0.08 g were randomly stocked into 12 tanks with a volume of 1 m3. At the end of the study, the changes observed in weight, feed conversion ratios (FCR) and survival rates were calculated as 6.69 ± 5.35-7.40 ± 5.47, 0.80 ± 0.18-0.88 ± 0.20 and 96.6 ± 1.51-100 ± 0.0, respectively. When the body composition of the control and treatment groups were compared, no statistically significant differences were observed between the protein and lipid values (p>0.05), except ash (p <0.05). Histological sections of intestinal tissue; the number of goblet cells was higher than that of the control group. The highest values were determined in the group supplemented 2% GroBiotic®-A. The highest microvillus length was found in the group added 1% GroBiotic®-A. It was detected an inverse relationship between microvillus length and contribution rates as the the GroBiotic®-A additive levels increased. Also, degeneration and necrosis was detected in hepatocyte cells of seabass juvenile fed with diets supplemented 2% and 3% GroBiotic®-A as well as increase of the number of fatty vacuoles in liver tissue due to the increase in the amount of GroBiotic®-A. In conclusion, when the growth parameters, body composition and histological data were evaluated together, the feeding group supplemented 1% GroBiotic®-A performed the best.
In present study, biochemical compositions (ash, lipid and protein) of five different macroalgae ((Green Macroalgae-GMA (Chaetomorpha linum and Caulerpa prolifera), Red Macroalgae-RMA (Pterocladiella capi-lacea), Brown Macroalgae-BMA (Sargassum vulgare and Ericaria amentacea)) and Angiosperm/Seagrass (Hal-ophila stipulacea) collected from Iskenderun Bay were investigated. The differences observed between biochemical compositions such as ash, lipid and protein of five macroalgae species and Angiosperm/Seagrass (Halophila stip-ulacea) were statistically significant (p<0.05). The lowest and highest ash, lipid and protein values of five macroalgae were 12.19±1.15% (Caulerpa prolifera)- 21.38±1.53% (Ericaria amentacea), 1.74±0.19% (Caulerpa prolifera)- 5.83±0.68% (Ericaria amentacea), 5.56±0.06% (Chaetomorpha linum)- 11.45±0.53% (Sargassum vulgare), respec-tively. Ash, lipid and protein values of Angiosperms/Seagrass (Halophila stipulacea) were determined as 14.56±2.08%, 3.16±0.48% and 8.11±0.07%, respectively. Protein value of Angiosperms/Seagrass (Halophila stipulacea) was higher than those of (GMA (Chaetomorpha linum and Caulerpa prolifera) but not RMA(Pterocladiella capillacea) and BMA (Sargassum vulgare and Ericaria amentacea)). Lipid value of Angio-sperms/Seagrass (Halophila stipulacea) was similar to RMA(Pterocladiella capillacea). In conclusion, the information of the biochemical compositions of five different macroalgae and Angiosperms/Seagrass (Halophila stipulacea) are important for the evaluation of potential sources for commercial and human consumption. In addition, biochemical compositions of tested macroalgae and seagrass could make important contributions to feed formulations and functional foods in future.