Healing of the uterus after cesarean section and myomectomy operation is clinically important. In this study, we aimed to investigate the effects of resveratrol (3,5,4'-o-trihydroxystilbene) on the wound healing process of the uterus in rats treated with resveratrol following full thickness injury of the uterus. Twenty-one female wistar albino rats were divided randomly into three groups (1) control group with no intervention (2) injury group with uterine full thickness injury (3) resveratrol group with uterine full thickness injury and treated with resveratrol. Resveratrol was injected by oral gavage at the doses of 0.5 mg/kg/day for 30 days following uterine full thickness injury. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) distributions were assessed using the immunohistochemical methods in tissue and ELISA methods in the tissue homogenate. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were evaluated with colorimetric method and malondialdehyde (MDA) levels also were measured using high performance liquid chromatography in the tissue homogenate. The effects of resveratrol on the uterine histology also were evaluated histologically with the light microscopy. Histological evaluation and immunohistochemical evaluations showed that treatment with a resveratrol significantly increased the thickness of the uterine wall and VEGF expression and decreased expression PDGF during wound healing. Biochemically, GPx and SOD activities were increased significantly after treatment with resveratrol. Additionally, resveratrol administration decreased MDA levels. These results showed that the antioxidant effects of resveratrol has been shown to have a positive influence on wound healing of the uterus.

The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the association of polymorphisms at the PLIN locus in adolescents with obesity and their connection with serum adipokines. A total of 308 children (206 obese, 66.8% and 102 healthy control, 33.2%) between the ages of 10-18 years were included into the study. PLIN gene analysis [PLIN 1, PLIN 4, PLIN 6, PLIN 5’UTR-1234 C > G and PLIN 10171 A/T] were studied by Real Time-PCR. Serum leptin, adiponectin, resistin and ghrelin levels were studied by ELISA method in both groups and their link with perilipin polymorphisms were analyzed. Serum leptin level was found significantly high in obese adolescents. Other adipokine levels were similar in both groups. The incidence of PLIN 1, PLIN 4, PLIN 5’UTR-1234 C > G and PLIN 10171 A/T minor and major alleles was similar in both groups. PLIN 6 T/T allele was determined significantly high in obese adolescents compared to that of control group. No correlation was detected between perilipin polymorphism and serum levels of adipokines. The PLIN 6 polymorphism of the perilipin gene may influence the risk of the obesity during adolescence. Retrospectively registered.

Reactive oxygen species induce oxidative stress that may contribute to endothelial dysfunction. In addition to endogen antioxidants, adding antioxidants to diet has been used to reduce oxidative-stress-induced endothelial dysfunction. Resveratrol is a natural phytoalexin which has many effects including antioxidant activity. The aim of this study was to investigate whether resveratrol could protect human coronary artery endothelial cells against in vitro oxidative-stress-mediated injury. Materials and methods: Human coronary artery endothelial cells were treated with varying concentrations of hydrogen peroxide for different durations and hydrogen peroxide plus varying concentrations of resveratrol. Then cell death was measured using lactate dehydrogenase release. Human coronary artery endothelial cells were exposed to hydrogen peroxide and resveratrol plus hydrogen peroxide for 15 min, 60 min, and 6 h. Reactive oxygen species was measured using a fluorometric assay. Results: Preincubation of cells with 10 and 50 \muM resveratrol for different periods caused a significantly decreased hydrogen-peroxide-induced cell death. Preincubation with resveratrol caused a significant decrease in reactive oxygen species production. Conclusion: Our results demonstrate that resveratrol protects human coronary artery endothelial cells, in vitro, against oxidative-stress-induced injury by decreasing cell death and reactive oxygen species production.

Amaç: Bu çalışmada, gebelikleri süresince yeşil çay ekstraktıyla beslenen sıçanların ve yavrularının karaciğer dokularında sSitokeratin-18 (SK-18) düzey ve ekspresyonunu değerlendirmek amaçlanmıştır.Yöntem: On sekiz adet Wistar albino gebe sıçan, iki gruba ayrıldı: kontrol grubu ve (oral gavaj ile 50 mg/kg yeşil çay ekstraktı verilenuygulanan) yeşil çay grubu. Yirmi bir günlük gebelikten sonra, her iki gruptaki anne sıçanların ve doğdukları ilk gün yavruların karaciğer dokuları çıkarıldı. Bu doku örneklerinde SK-18 ekspresyonu ve düzeyi immünohistokimyasal olarak ve enzime bağlı immünosorbent analiz (ELISA) ile değerlendirildi.Bulgular: İki grupta da maternal dokularda santral venlerin çevresindeki hepatositlerin hücre zarları yakınında kuvvetli SK-18 immünoreaksiyonu gözlendi. Kontrol grubu yenidoğan dokularında santral ven çevresindeki hepatositlerde zayıf SK-18 immünoreaksiyonu gözlenirken, yeşil çay grubunda hepatositlerin hücre zarı yakınında oldukça kuvvetli SK-18 immünoreaksiyonu gözlendi. Biyokimyasal incelemede de, maternal SK-18 düzeyleri her iki grupta da yüksek olup birbirinden istatistiksel olarak anlamlı farklılık göstermezken, neonatal SK-18 düzeyleri kontrol grubuna kıyasla yeşil çay grubunda anlamlı biçimde daha yüksekti.Sonuç: İmmünohistokimya ve ELISA sonuçlarımız gebelik süresince maternal yeşil çay tüketiminin yenidoğan karaciğerinde hücre hasarına neden olabileceğini düşündürmektedir.