Agaricus bisporus, the most widely consumed mushroom species globally, was the focus of this study. Mushrooms are widely recognized as important sources of dietary fiber, proteins, carbohydrates, lipids, vitamins and minerals. However, they also have the capacity to accumulate toxic metals such as lead, cadmium, and mercury. Due to the presence of polysaccharides, peptides, essential amino acids, glycoproteins, nucleosides, triterpenoids, fatty acids and lectins, Agaricus bisporus has been reported to have antioxidant, antimicrobial, anti-hyper-cholesterolemic, antidiabetic, antihypertensive and hepatoprotective activities. We aimed to determine the mineral profile of Agaricus bisporus samples available on the Romanian market using X-ray fluorescence (XRF) spectrometry. The results regarding the mushrooms samples minerals content (mg/kg dry weight), obtained by using X-ray fluorescence (XRF) spectrometry, are ranging between: K (31443-50579), Ca (1229-4838), Fe (337-580), Mn (105-130), Zn (113-312), Cu (25-86), Se (0-11). The analysis of obtained data was made using Excel, Statistica 11 and MVSP programs. These findings are consistent with previous studies indicating that potassium is the predominant mineral in mushrooms, followed by calcium. Therefore, regular mushroom consumption can contribute to meeting the daily requirements for these essential elements, alongside other nutritional benefits.