Salt stress induces cytotoxicity at the cellular level, influencing the vacuolization process, disrupting mitotic division, and thus inhibiting plant growth. The results for a range of species used in agriculture have shown that high soil salt levels affect germination, chlorophyl content and yield. In this study, an experiment was carried out in the laboratory using NaCl concentration treatments of 0, 100, 125 and 150 mM on sunflower seeds of the inbred line HA-89 obtained from the USDA gene bank. For the experiment, the seeds were germinated in a salt solution and analyzed cytologically by calculating the mitotic index, chromosomal aberration index, provacuolar index and vacuolization index. Following our cytological studies, we observed that the vacuolization phenomenon was caused by salt stress and progressively accentuated by the salt concentration levels and exposure times. The formation of vacuolized cells is due to the fusion of provacuoles, which contributes to a uniform or non-uniform distribution of genetic material around them. According to our results, the vacuolization index showed high values depending on the NaCl concentration and stress exposure time. Similarly, high salt concentrations significantly decreased the mitotic index and increased the chromosomal aberration index. The effect of salt stress causes cell vacuolization, a decrease in the mitotic index and an increase in the number of chromosomal aberrations in meristematic tissues, inhibiting growth and development and consequently leading to a reduction in productivity per unit area.

Applications of the Drosophila melanogaster (D.m.) research model have an important means both for genetic investigations and for the study of metal toxicity, because D.m. has physiological mechanisms comparable to those in human organisms. In this research, the toxic effect of lead (Pb²⁺) and copper (Cu²⁺) on four D.m. genotypes—the wild genotype (Oregon-R, used as control) and three mutant genotypes (white, brown, and white-vestigial)—was compared. Five replicates were made to observe the development progress of monitorized genotypes exposed to five different concentrations 0, 0.50, 0.75, 1.00, and 2.00 mM of copper (CuSO₄) and lead Pb(C₂H₃O₂)₂. Proliferation rates of larvae, pupae, and adults depend on genetic factors, metals used (copper or lead), and their concentrations. The white-vestigial mutant genotype showed the greatest sensitivity at IC₅₀ concentration (inhibition of proliferation of more than 50% compared with the control sample) at doses of 1.00 mM for Cu²⁺ and 2.00 mM for Pb²⁺. In contrast, the control genotype (Oregon-R) showed only an inhibition IC₅₀ concentration of 2.00 mM for Cu²⁺. The white-vestigial mutant genotype showed the greatest sensitivity at IC₅₀ concentration (inhibition of proliferation of more than 50% compared with the control sample) at doses of 1.00 mM for Cu²⁺ and 2.00 mM for Pb²⁺. In contrast, the control genotype (Oregon-R) showed an inhibition at the IC₅₀ concentration of 2.00 mM for Cu²⁺. The results conclude that (i) the dose influences the prolificacy rate in a directly proportional way, (ii) the comparative analyses between Cu²⁺ and Pb²⁺ revealed a more acute effect of Cu²⁺, and (iii) differentiated prolificacy values according to genotypes were recorded. Those reflect the importance of using D.m. as a research model in the comparative studies of the interactions between genetic factors and metal toxicity. Also, this study provides significant information on non-toxic maximum doses for organisms.

The improvement of Lavandula angustifolia Mill. seed germination represents a crucial step towards the development of eco-biotechnological solutions for the sustainable propagation of aromatic plants. This study evaluated the effects of four biostimulant formulations, namely Amino 16 (amino acid-based), Razormin (humic–fulvic complex), Germinoseed (phytoextract-based), and Atonik (nitrophenolate), together with a non-treated control, on the germination efficiency and early growth of nine Lavandula genotypes under controlled laboratory conditions. A factorial design (9 × 5) with four replications was applied, and multiple germination indices were calculated. Data were analysed using a two-way ANOVA with genotype and treatment as main factors. Results indicated significant genotype-dependent responses. Amino 16 and Razormin markedly increased germination percentage, speed of emergence, and seedling vigour, achieving up to 100% germination in responsive genotypes such as ‘Ellagance Snow’ and ‘Blue Spear’. Correlation and clustering analyses revealed strong links between seed size, germination rate, and seedling development, suggesting a possible synergistic role of amino and humic substances in stimulating metabolic activation during germination. These findings demonstrate that eco-friendly biostimulants function as effective biotechnological activators of seed physiology, supporting low-input propagation systems and the transition toward a circular green bioeconomy.

Salt-affected soils constrain wheat production in many countries. It is estimated that more than 831 million hectares (Mha) are salt affected globally, with 10 Mha of cultivated land lost annually. Salinity adversely affects wheat grain yield and grain quality traits. Wheat genotypes show wide variation for salinity stress tolerance. The main objective of this study was to evaluate the performance and stability of spike traits in 14 winter wheat varieties with different origin, in order to identify the varieties that can exploit the growing conditions of saline soils. The wheat material was tested in nine environments as a combination of three years and three fertilization treatments (urea, calcium nitrate, ammonium nitrate), using a split-plot design with three replications. At maturity plant height, spike length, grains number per spike, and spike yield were determined. The significant effect of genotype × environment interaction on the phenotypic expression of different traits indicates differential response of the genotypes across the testing environments, thus offering the possibility of identifying some genotypes of interest. The Romanian varieties ‘Alex’ and ‘Glosa’ present the highest productivity of spike associated with an average stability, being specifically adapted to favourable rainfall conditions, achieving above-average yields in less favourable conditions. The varieties ‘Cerere’, ‘Genesi’, ‘Alex’ and ‘Glosa’ can be selected for crossing and included in wheat breeding programs for the development of stable cultivars adapted to saline soils.

Drosophila melanogaster serves as a important research model both for genetic investigations and for the study of metal toxicity, facilitating the elucidation of physiological mechanisms comparable to those of human organisms. In our research, we evaluated the toxicity effect resulting from exposure to various concentrations of lead and copper on the prolificity rates throughout the life cycle (egg-adult) of four genotypes of Drosophila melanogaster: wild-type (control), white, brown, and white-vestigial. During our study, the prolificity rates were examined across three repetitions under the impact of exposure to concentrations of 0.50, 1.00, 2.00, and 4.00 mM of copper (CuSO₄) and lead (Pb(C₂H₃O₂₎₂). Prolificity rates throughout the life cycle exhibited variations as a direct consequence of genetic factors, the concentration of exposure, and the specific type of metal, either copper or lead. The mutant white-vestigial genotype revealed an IC₅₀ concentration for prolificity inhibition at lower doses of 2.00 mM for copper and 4.00 mM for lead, in contrast to the control genotype (wild-type), which exhibited an inhibition concentration rate >IC₅₀ of 4.00 mM only in the case of copper. Our results concluded that the (i) dose influences the prolificity rate in a directly proportional manner, (ii) comparative analyses between copper and lead revealed that copper displayed toxicity across all genotypes within the concentration range of 0.50 mM to 2.00 mM. In contrast, lead exhibited toxicity within the concentration range of 1.00 mM to 4.00 mM, highlighting a (iii) more acute toxicity characteristic in the case of copper. Thus, the results of this research reflect the importance of using Drosophila melanogaster as a genetic model in the comparative study of the interaction between genetic factors and the toxicity of metals, offering significant insights into monitoring their impact and defining the maximum permissible doses on organisms.

Drought is one of the main factors that limit the production level, causing harvest losses of over 50%, depending on the duration and intensity of the water deficit. Production losses due to drought can be increased by interaction with other stress factors such as high temperatures. Many selection indices based on seed yield were developed and used for the evaluations of common bean germplasm for drought tolerance. The objectives of this study were to evaluate the yield response to drought stress for six Romanian varieties of common bean using 14 tolerance indices, to establish the efficiency of these selection criteria and the varieties with good drought tolerance. The biological material for this study was composed by six Romanian varieties of common bean studied in Timisoara during 2022-2024, under different levels of drought stress. Based on the obtained results, the following indices: yield index, drought resistance index, stress susceptibility percentage index, stress tolerance index, geometric mean productivity, mean productivity, shows very strong positive correlations with both yield under stress and non-stress conditions, indicating that they can be used with high efficiency for the selection of genotypes with superior yields under different water stress conditions. ‘Star’ and ‘Avans’ varieties showed a moderate tolerance to drought, achieving reasonable levels of plant yield under conditions with and without water stress.