Faba bean (Vicia faba L.) is an important pulse crop traditionally used for human nutrition and animal feeding. With a high protein content ranging from 24% to 35% of seed dry matter, considerable amounts of globulins, essential amino acids and minerals, faba bean is today an important source meeting the growing global demand for nutritious food. The objective of study was to investigate the variability of nine phenological, phenotypical and yield related traits in 220 faba bean accessions in multi-location trials across four representative European regions. Nine field trials were carried out from 2018 till 2020 in four representative European locations (Spain, Finland, Belgium and Serbia) using an augmented p-rep design containing 20 replicated checks. Significant differences among genotypes and environments were detected, being the genotype x environment interaction (GEI) the major source of variation in five of the nine evaluated traits. The “which-won-where” analyses identified two mega-environment namely South European mega environment (SE-ME) and North European mega environment (NE-ME), while the best performing and most stable genotypes according to the nine traits were identified using “means vs stability” analyses. According to the highest trait value in each mega environment several winning genotypes were identified showing better performances than some commercial varieties (controls) or checks. Our results suggest that the geographical locations falling into each mega-environment can be used as faba bean test locations. The genotype ranking for the multi-trait stability index (MTSI) revealed that the most stable and best ranking genotypes in SE-ME are G018, G086, G081, G170 and G015 while in the north mega-environment are G091, G171, G177 (Merkur), G029 and G027. Hierarchical cluster analysis and principal component analyses showed a clear correlation between the traits analysed and the botanical type. These findings indicate that botanical type is one of the most significant factors affecting development in any environment, and it must be taken into account in faba bean breeding activities. The information derived from this study provides a chance for breeding new resilient faba bean cultivars adapted to different agroecological European regions, a critical point for addressing Europe’s reliance on protein imports and enhancing sustainable agriculture practices.

Due to historical background of vegetation development, geographical position, climate and relief, Serbia represents one of the 158 world biodiversity centres, based upon the number of plant species and territory size (biodiversity index 0.72). Large areas in Serbia are under natural grasslands and pastures, composed of forage grass species, and important as source of natural plant genetic diversity and germplasm for breeding. These eco-systems represent basic prerequisites for sustainable forage production, but very low potential of them is utilized and genetic resources are not protected. Family Poaceae is present in Serbia flora with 70 genera and among them from the aspect of forage production and quality, the most important are perennial Festuca, Lolium, Dactylis, Phleum, Bromus, Arrhenatherum, Poa and Agrostis species. Most of these grasses have been bred in Serbia and lot of cultivars were released. These cultivars contain autochthonous Serbian material and represent great and important resource of genetic variability. Therefore, collecting of new samples which are acclimatised to local eco-geographical conditions and including them in plant ex situ gene bank is of exceptional importance for further utilization in different plant breeding programmes as well as genetic resources protection. These autochthonous populations have natural variability and very often have satisfactory yielding performance in comparison with introduced cultivars, which referred them for direct phenotypic selection for cultivars release. Broadening of forage grasses genotypes collection is permanent objective of Serbian scientists. Collected accessions are being characterized and evaluated for important phenological, morphological and agronomical traits. In this paper genetic resources of forage grass species, their diversity and potentials, state of the grasses gene banks, as well as possibility for breeding of new cultivars has been analysed.