The urbanization-driven surge in kitchen waste necessitates optimized dry anaerobic digestion (DAD; total solids > 15%). Despite its valorization potential, this technology requires efficiency improvements due to mass transfer constraints. This study evaluated TS effects (15%, 20%, or 25%) on methane production. The TS = 20% system achieved peak cumulative methane yield (405.73 ± 11.71 mL/gVS), exceeding TS = 15% (348.09 ± 12.19 mL/gVS) and TS = 25% (293.08 ± 3.55 mL/gVS). This optimization was attributable to synergistic maintenance of metabolic equilibrium through autonomous pH recovery, rapid VFAs degradation, and enhanced TAN tolerance. Conversely, TS = 25% exhibited impaired mass transfer efficiency under high solids, causing VFAs accumulation, ammonia toxicity, and progressive pH decline to 7.5, indicating system destabilization. Organic degradation analysis confirmed superior conversion efficiency in TS = 20% through dynamic SPS–SPN equilibrium. Microbial analysis revealed enhanced metabolic efficiency via synergistic interactions between acetoclastic and hydrogenotrophic methanogens in TS = 20%. This research provides technical parameters for optimizing methane production in kitchen waste DAD systems.
Wastewater treatment plants (WWTPs) have been confirmed as reservoirs of antibiotic resistance genes (ARGs). This study systematically investigated the distribution patterns of ARGs across different treatment units in municipal WWTPs, along with the environmental drivers, dissemination characteristics, and exposure risks of aerosol-borne ARGs in aerated tank environments. The results revealed a high compositional similarity in aerosol-borne ARGs across the sampling sites, with multidrug ARGs predominating at an average relative abundance of 52%, followed sequentially by tetracycline (11%), MLS (10%), and glycopeptide resistance genes (7%). The diffusion of aerosol-borne ARGs is significantly influenced by environmental factors including temperature, relative humidity, wind speed, and total suspended particulate (TSP) concentration, with temperature being the most dominant factor affecting the dispersion of ARGs. The atmospheric dispersion model demonstrates that aerosol-borne ARGs decay with increasing downwind distance, showing potential for transport from aeration tanks to locations exceeding 1500 m along the prevailing wind direction. Both within wastewater treatment units and downwind areas, adult males had higher respiratory exposure doses but lower skin contact doses compared to females, with respiratory doses exceeding skin contact by 3–4 orders of magnitude. This study highlights the potential health risks posed by aerosol-borne ARG transmission from WWTP operations.