Surface-Enhanced Raman Scattering (SERS) is a technique currently widely used in the identification and quantification of organic and biological molecules at low concentrations, in which an important application is the detection of pesticide residues in food. To accomplish this task, SERS substrates with high Raman enhancement factor and good reproducibility are required. One of the most commonly used SERS substrates is the SERS substrate made of silver nanoparticles immobilized on a solid substrate. In this report we first present the results of electrochemical deposition of silver nanoparticles on the silicon surface using ethanol electrolyte solution. Thanks to both factors, electrochemical deposition (instead of electroless) and ethanol electrolyte (instead of aqueous), under optimal conditions, on the surface of silicon a monolayer of silver nanoparticles grew, which are uniform in shape and size and are arranged very close to each other with nanometer separation. Next we report on the use of fabricated arrays of silver nanoparticles in the role of a SERS substrate. To test the performance of the SERS substrate, the probe molecules used were molecules of difenoconazole, a well-known fungicide. Results showed that difenoconazole could be detected with a detection limit of 0.023 ppm (5.6 × 10⁻⁸ M).

Wool roll-like silver nanoflowers of different sizes were obtained by reducing AgNO ₃ with ascorbic acid in the presence of citric acid in a mixed ethanol/water solution with different volume ratios.