Background: The aim of this study was to evaluate effect of luteolin nanoparticles cream on wound healing in rabbit skin. Martial and Methods: Thirty male rabbits were used in this study. Wound in skin was induced in all rabbits and divided into 5 groups. Group 1: no wound carried out (negative control), Group2: (positive control) dressing wounds by cream only while Groups 3,4,5: dressing wounds by luteolin nanoparticles cream (1%, 2%, 4%), respectively. Treatment was repeated every day. The wound size, tissue type observation assessment were measured at 1st, 3th, 7th days of the experimental procedure. Results: At 3th day no significant difference between negative and positive control groups was observed but there are significantly difference between all treated groups of luteolin nanoparticles cream at (1%, 2%, 4%) in comparison to positive control and negative control groups . At day 7 there were significant difference in wounds size between groups treated with luteolin nanoparticles in different concentrations (9.3±1.3, 7.3±0.6 and 4.0±1.1),respectively and between negative control groups whereas no significant difference within groups treated with luteolin nanoparticles cream in 1%, 2% and 4% (9.3±1.3,7.3±0. 6 and 4.0±1.1 mm), respectively. Clinical finding of the tissue type that present in the wound showed that epithelization in all groups treated with luteolin nanoparticles in different concentrations in comparison to negative and positive control. Conclusions: Topical application luteolin nanoparticles cream in different concentrations accelerate skin wounds healing in rabbits.

Aspirin are commonly used analgesic, anti-inflammatory, and anti-pyretic drug in medicine, oral route is the most common one for drug administration as a result it will produce different adverse effects like peptic ulcer, nephropathy, and thrombocytopenia even with low and continuous therapeutic dose, so the alternative topical route is preferable with minimal adverse effects and effective concentration.Therefore, in the present study was to investigate whether the antinociceptive property of aspirin would enhance if used aspirin as nanoparticles after preparing it in several forms (gel, cream and ointment). Thirty-two healthy male mice weighing 30-35 gm. were used in the present study. The animals were divided as a randomized design. Each mouse was treated topically. All drug concentration of aspirin was prepared using gel, cream and ointment as vehicle and topically application on fore and hind paw of experimental animals. Pain was induced by application of hot plate for assessment of latency of pain stimulus. Time from placement to jumping or hind paw licking was recorded as latency of response. The result showed that the median effective concentration (EC50) for analgesic effect of aspirin (gel, cream, and ointment) were 0.848, 0.958 and 1.00% respectively while these EC50s were decrease when used nanoparticles aspirin (gel, cream and ointment) to 0.72, 0.657, and 0.701% respectively. In conclusion, topical applied of aspirin will produce effective therapeutic antinociceptive effects in mice although gel preparation produce a better response followed by cream, then ointment due to pharmacokinetic properties. Also nanoparticle preparation will produce superior response in all forms, whether Nano aspirin is prepared in gel form, cream or ointment.