This Technical Note presents the design and implementation of a low-cost modular growth chamber developed to keep mother plants under controlled environmental conditions for vegetative propagation. The system was conceived as an accessible alternative to expensive commercial equipment, offering reproducibility and adaptability for small-scale and research-based cultivation. The proposed chamber integrates thermal insulation, LED lighting, forced ventilation through the implementation of extractors, a recirculating irrigation system with double filtration, and a sensor-based environmental monitoring platform operated via an Arduino UNO microcontroller. The design features a removable tray that serves as a support for the mother plant, an observation window covered by a movable dark acrylic that prevents the passage of external light, and a vertical structure that facilitates optimal space utilization and ergonomic access. Functionality was conducted using a Stevia rebaudiana Bertoni mother plant maintained for 30 days under monitored conditions. Environmental parameters—temperature, relative humidity, and illuminance—were recorded continuously. The plant showed vegetative development through new shoot emergence and the growth in height of the plant, and despite a loss in foliage expansion, it confirmed the chamber’s capacity to support sustained growth. Although no statistical replication or control group was included in this preliminary evaluation, the system demonstrates technical feasibility and practical utility. This chamber provides a replicable platform for future experimentation and propagation studies. Complete technical specifications, schematics, and component lists are provided to enable replication and further development by other researchers. The growth chamber design aligns with the goals of open-source agricultural innovation and supports knowledge transfer in controlled-environment plant propagation technologies.

This study presents the design, construction, and evaluation of a modular chamber for the vegetative propagation of cuttings, capable of simultaneously accommodating three plant cuttings at equidistant positions within a compact volume of 40 cm × 45 cm × 15 cm. It integrates distributed sensing using DHT22 temperature and relative humidity sensors, a BH1750 light sensor, and an MQ-2 gas/smoke detection sensor; programmable lighting via four NeoPixel WS2812B boards arranged in two parallel rows; and an ESP32 microcontroller responsible for processing, wireless communication, and threshold-based feedback control of environmental variables. The system was continuously monitored for four weeks to characterize the internal foliar microclimate in relation to external environmental conditions. Internal temperatures ranged from a weekly average of 25.26 °C to 28.71 °C, while relative humidity in the foliar zone varied between 28.99% and 41.24%. The lighting system provided stable and repeatable illuminance conditions, with weekly averages reaching up to 111.10 lux, and the gas sensor enabled continuous detection of transient variations in gas concentration within the chamber. The results demonstrate the feasibility of the proposed chamber as a compact, modular, and replicable platform for environmental data acquisition and experimental control in future studies on plant cutting propagation, biological rooting performance was not evaluated in the present study, therefore, the reported results primarily validate the technical and environmental performance of the developed prototype.