Conventional methods for transporting biological materials typically use dry ice or ice for preservation but often overlook important aspects of temperature monitoring and metrological control. These methods generally do not include temperature sensors to track the thermal conditions of the materials during transport, nor do they apply essential metrological practices such as regular sensor calibration and stability checks. This lack of precise monitoring poses significant risks to the integrity of temperature-sensitive biological materials. This study presents a statistical analysis of DS18B20 digital temperature sensors used in an experimental refrigeration system based on thermoelectric modules. The aim was to verify sensor consistency and investigate sources of measurement error. The research was motivated by a prior phase of study, which revealed significant discrepancies of approximately 3 °C between experimental temperature data and numerical simulations. To investigate a potential cause, we conducted a case study analyzing measurements from three identical temperature sensors (same model, brand, and manufacturer). Statistical analyses included ANOVA (analysis of variance) and Tukey’s test with a 95% confidence interval. Since the data did not follow a normal distribution (p-value < 0.05), non-parametric methods such as the Kruskal–Wallis and Levene’s procedures were also applied. The results showed that all sensors recorded statistically significant different temperature values (p-value < 0.05). Although experimental conditions were kept consistent, temperature differences of up to 0.37 °C were observed between sensors. This finding demonstrates an inherent inter-sensor variability that, while within manufacturer specifications, represents a source of systematic error that can contribute to larger discrepancies in complex systems, highlighting the need for individual calibration.