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Zahoor Ul Hassan
Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Doha P.O. Box 2713, Qatar

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Journal article
Published: 06 August 2021 in Chemico-Biological Interactions
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Ochratoxin A (OTA), an important fungal metabolite in foods and feeds has been shown to induce oxidative stress and cellular injuries to human and animal subjects. This study was designed to investigate the mode of action of a biological modifier Trichosporon mycotoxinivorans (TM), against OTA-mediated oxidative stress and tissue toxicity on broiler chickens. The birds were offered diets supplemented with OTA (0.15 and 0.3 mg/kg feed) and/or TM (0.5, 1.0 g/kg) for 42 days of age, and blood and tissue samples were collected to examine the oxidative stress, biochemical and histopathological parameters. Dietary OTA at all the tested levels induced the hepatic and renal tissue injury as indicated by significant decreased total antioxidant capacity in these organs along with significant decreased (p ≤ 0.05) serum concentrations of total proteins and albumin. The serum concentrations of alanine aminotransferase (ALT) and urea were significantly increased, and these observations were further supported by degenerative changes and increased relative weights of liver and kidneys. The dietary supplementation of TM at both tested levels relieved the detrimental impact of 0.15 and 0.3 mg OTA/kg on the studied parameters. The results of the study demonstrated that dietary TM significantly protects broiler chickens by reducing OTA-induced oxidative damage and tissue injury.

ACS Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Zahoor Ul Hassan. Dietary Trichosporon mycotoxinivoron modulates ochratoxin-A induced altered performance, hepatic and renal antioxidant capacity and tissue injury in broiler chickens. Chemico-Biological Interactions 2021, 347, 109614 .

AMA Style

Sheraz Ahmed Bhatti, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Zahoor Ul Hassan. Dietary Trichosporon mycotoxinivoron modulates ochratoxin-A induced altered performance, hepatic and renal antioxidant capacity and tissue injury in broiler chickens. Chemico-Biological Interactions. 2021; 347 ():109614.

Chicago/Turabian Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Zahoor Ul Hassan. 2021. "Dietary Trichosporon mycotoxinivoron modulates ochratoxin-A induced altered performance, hepatic and renal antioxidant capacity and tissue injury in broiler chickens." Chemico-Biological Interactions 347, no. : 109614.

Journal article
Published: 22 February 2021 in Environmental Technology & Innovation
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Mycotoxins, the metabolites of toxigenic fungi are important contaminants of food and agriculture industry throughout the world. Among the different strategies to minimize the mycotoxins synthesis and decontamination of food; yeasts and their derivatives are used as efficient biological control agents. In this study, cell walls (CW) of six yeast strains; two commercial baking {Saccharomyces cerevisiae (Sc 1 and Sc2)}, two low-fermenting (Candida intermedia and Lachancea thermotolerans), and two non-fermenting (Cyberlindnera jadinii and Candida friedrichii) were explored to inhibit A. flavus growth, aflatoxin (AF) synthesis and remove mycotoxins from contaminated buffers (at pH 3, 5 and 7) and milk. The volatiles of non-fermenting yeasts showed a significant inhibitory effect on of A. flavus growth (up to 79%) and aflatoxin synthesis potential. The highest mycotoxins binding activities of Sc strains were noted against ochratoxin A (92%), AFB2 (66%), AFG2 (59%) and AFB1 (31%). The highest and lowest binding activities occurred at pH 7 and pH 3, respectively. On the other hand, all yeast CWs significantly (44%–54%) reduced aflatoxin M1 (AFM1) from contaminated milk. These results show clearly that yeasts (particularly Sc) can be used as efficient biocontrol and decontamination agents in food industry or the management of mycotoxin contamination.

ACS Style

Zahoor Ul Hassan; Roda Al Thani; Fathy A. Atia; Mohammed Alsafran; Quirico Migheli; Samir Jaoua. Application of yeasts and yeast derivatives for the biological control of toxigenic fungi and their toxic metabolites. Environmental Technology & Innovation 2021, 22, 101447 .

AMA Style

Zahoor Ul Hassan, Roda Al Thani, Fathy A. Atia, Mohammed Alsafran, Quirico Migheli, Samir Jaoua. Application of yeasts and yeast derivatives for the biological control of toxigenic fungi and their toxic metabolites. Environmental Technology & Innovation. 2021; 22 ():101447.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al Thani; Fathy A. Atia; Mohammed Alsafran; Quirico Migheli; Samir Jaoua. 2021. "Application of yeasts and yeast derivatives for the biological control of toxigenic fungi and their toxic metabolites." Environmental Technology & Innovation 22, no. : 101447.

Journal article
Published: 02 December 2019 in Toxins
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Mycotoxins are secondary metabolites produced by certain filamentous fungi, causing human and animal health issues upon the ingestion of contaminated food and feed. Among the safest approaches to the control of mycotoxigenic fungi and mycotoxin detoxification is the application of microbial biocontrol agents. Burkholderia cepacia is known for producing metabolites active against a broad number of pathogenic fungi. In this study, the antifungal potential of a Qatari strain of Burkholderia cepacia (QBC03) was explored. QBC03 exhibited antifungal activity against a wide range of mycotoxigenic, as well as phytopathogenic, fungal genera and species. The QBC03 culture supernatant significantly inhibited the growth of Aspergillus carbonarius, Fusarium culmorum and Penicillium verrucosum in PDA medium, as well as A. carbonarius and P. verrucosum biomass in PDB medium. The QBC03 culture supernatant was found to dramatically reduce the synthesis of ochratoxin A (OTA) by A. carbonarius, in addition to inducing mycelia malformation. The antifungal activity of QBC03’s culture extract was retained following thermal treatment at 100 °C for 30 min. The findings of the present study advocate that QBC03 is a suitable biocontrol agent against toxigenic fungi, due to the inhibitory activity of its thermostable metabolites.

ACS Style

Randa Zeidan; Zahoor Ul-Hassan; Roda Al-Thani; Quirico Migheli; Samir Jaoua. In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius. Toxins 2019, 11, 700 .

AMA Style

Randa Zeidan, Zahoor Ul-Hassan, Roda Al-Thani, Quirico Migheli, Samir Jaoua. In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius. Toxins. 2019; 11 (12):700.

Chicago/Turabian Style

Randa Zeidan; Zahoor Ul-Hassan; Roda Al-Thani; Quirico Migheli; Samir Jaoua. 2019. "In-Vitro Application of a Qatari Burkholderia cepacia strain (QBC03) in the Biocontrol of Mycotoxigenic Fungi and in the Reduction of Ochratoxin A biosynthesis by Aspergillus carbonarius." Toxins 11, no. 12: 700.

Journal article
Published: 09 October 2019 in ACS Omega
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The present study was designed to investigate the antagonistic activity of Bacillus licheniformis BL350-2 against mycotoxigenic strains of Aspergillus and Penicillium. In vitro coincubation for 5 days indicated Aspergillus westerdijkiae BA1 as the most sensitive strain, with a growth inhibition of 62%, followed by A. carbonarius MG7 (60%), Penicillium verrucosum MC12 (53%), A. niger MC05 (50%), A. flavus CM5 (49%), A. parasiticus SB01 (47%), and A. ochraceus MD1 (44%). Likewise, the majority of the tested strains on exposure to bacterial volatiles showed complete inhibition of mycotoxin synthesis. In vivo assays on maize ears resulted in 88% reduction in A. flavus CM5 growth and complete inhibition of fungal sporulation and aflatoxin accumulation. The GC–MS-based volatile profile showed 3-methyl-1-butanol as the most abundant compound. The findings of the present study advocate that B. licheniformis BL350-2 is suitable as a biocontrol agent against mycotoxigenic fungi, at least during storage of cereal grains.

ACS Style

Zahoor Ul Hassan; Roda Al Thani; Hajer Alnaimi; Quirico Migheli; Samir Jaoua. Investigation and Application of Bacillus licheniformis Volatile Compounds for the Biological Control of Toxigenic Aspergillus and Penicillium spp. ACS Omega 2019, 4, 17186 -17193.

AMA Style

Zahoor Ul Hassan, Roda Al Thani, Hajer Alnaimi, Quirico Migheli, Samir Jaoua. Investigation and Application of Bacillus licheniformis Volatile Compounds for the Biological Control of Toxigenic Aspergillus and Penicillium spp. ACS Omega. 2019; 4 (17):17186-17193.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al Thani; Hajer Alnaimi; Quirico Migheli; Samir Jaoua. 2019. "Investigation and Application of Bacillus licheniformis Volatile Compounds for the Biological Control of Toxigenic Aspergillus and Penicillium spp." ACS Omega 4, no. 17: 17186-17193.

Journal article
Published: 01 October 2019 in Food and Chemical Toxicology
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Ochratoxin A (OA), the secondary metabolite of certain Aspergillus and Penicillium species, is one of the potent biological immune-suppressor. The present study was designed to explore the in-vivo efficacy of Trichosporon mycotoxinivorans (TR); yeast strain isolated from the hindgut of the termite Mastotermes darwiniensis, against the immunotoxicity of OA in broiler birds. For this purpose, broiler chicks were offered diet added with TR (0.5, 1.0 or 2.0 g/kg feed) and/or OA (0.15, 0.3 or 1.0 mg/kg feed) for 42 days. Dietary OA at all levels, resulted in significant reduction (p ≤ 0.05) in the immune response of broiler birds as recorded by vacuolation and darkly stained pyknotic nuclei in bursa of Fabricius and thymus, humoral immune responses to sheep red blood cells (SRBC), in-vivo lymphoproliferative response to Phytohemagglutinin-P (PHA-P) and mononuclear phagocytic system function assay. Addition of TR in broiler diet significantly reduced (p ≤ 0.05) the immunotoxicity of OA at 0.15 and 0.30 mg/kg; however, against higher dietary level of OA (1.0 mg/kg), a partial protection was observed. Feeding TR alone had no immunomodulatory effect at any of tested level. Dietary addition of TR is proposed as an approach to combat the OA mediated immunological damages in broiler birds.

ACS Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Zahoor Ul Hassan. Impact of dietary Trichosporon mycotoxinivorans on ochratoxin A induced immunotoxicity; In vivo study. Food and Chemical Toxicology 2019, 132, 110696 .

AMA Style

Sheraz Ahmed Bhatti, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Zahoor Ul Hassan. Impact of dietary Trichosporon mycotoxinivorans on ochratoxin A induced immunotoxicity; In vivo study. Food and Chemical Toxicology. 2019; 132 ():110696.

Chicago/Turabian Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Zahoor Ul Hassan. 2019. "Impact of dietary Trichosporon mycotoxinivorans on ochratoxin A induced immunotoxicity; In vivo study." Food and Chemical Toxicology 132, no. : 110696.

Journal article
Published: 10 July 2019 in International Journal of Food Microbiology
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The use of yeast-derived volatile organic compounds (VOCs) represents a promising strategy for the biological control of various plant pathogens, including mycotoxin-producing fungi. Previous studies demonstrated the efficacy of the low-fermenting yeast Candida intermedia isolate 253 in reducing growth, sporulation, and ochratoxin A biosynthesis by Aspergillus carbonarius MPVA566. This study aimed to investigate whether the inhibitory effect of the yeast volatilome is solely attributable to 2-phenylethanol, its major component, or if a synergistic effect of all volatilome components is required to achieve an effective control of the fungal growth and metabolism. Microbiological methods, HPLC measurements and a UPLC-MS/MS approach were used to investigate the metabolic profile of A. carbonarius MPVA566 at different growing conditions: standard incubation (control), exposed to C. intermedia 253 volatilome, and incubation in the presence of 2-phenylethanol. Both yeast volatilome and 2-phenylethanol succeeded in the macroscopic inhibition of the radial mycelial growth, along with a significant reduction of ochratoxin A production. Functional classification of the fungal proteome identified in the diverse growing conditions revealed a different impact of both yeast VOCs and 2-phenylethanol exposure on the fungal proteome. Yeast VOCs target an array of metabolic routes of fungal system biology, including a marked reduction in protein biosynthesis, proliferative activity, mitochondrial metabolism, and particularly in detoxification of toxic substances. Exposure to 2-phenylethanol only partially mimicked the metabolic effects observed by the whole yeast volatilome, with protein biosynthesis and proliferative activity being reduced when compared with the control samples, but still far from the VOCs-exposed condition. This study represents the first investigation on the effects of yeast-derived volatilome and 2-phenylethanol on the metabolism of a mycotoxigenic fungus by means of proteomics analysis. 2-Phenylethanol (PubChem CID: 6054); ochratoxin-A (PubChem CID: 442530); sodium dodecyl sulfate (PubChem CID: 3423265); dithiothreitol (PubChem CID: 446094); phenylmethylsulfonyl fluoride (PubChem CID: 4784); iodoacetamide (PubChem CID: 3727); ammonium bicarbonate (PubChem CID: 14013); acetic acid (PubChem CID: 176); and acetonitrile (PubChem CID: 6342).

ACS Style

Bruno Tilocca; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. A proteomic investigation of Aspergillus carbonarius exposed to yeast volatilome or to its major component 2-phenylethanol reveals major shifts in fungal metabolism. International Journal of Food Microbiology 2019, 306, 108265 .

AMA Style

Bruno Tilocca, Virgilio Balmas, Zahoor Ul Hassan, Samir Jaoua, Quirico Migheli. A proteomic investigation of Aspergillus carbonarius exposed to yeast volatilome or to its major component 2-phenylethanol reveals major shifts in fungal metabolism. International Journal of Food Microbiology. 2019; 306 ():108265.

Chicago/Turabian Style

Bruno Tilocca; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. 2019. "A proteomic investigation of Aspergillus carbonarius exposed to yeast volatilome or to its major component 2-phenylethanol reveals major shifts in fungal metabolism." International Journal of Food Microbiology 306, no. : 108265.

Research article
Published: 23 May 2019 in Environmental Science and Pollution Research
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Cadmium is an important widely distributed heavy metal in the environment due to its several industrial uses, while milk thistle is an important herb and is a source of several antioxidant particularly silymarin which is a pharmacological active substance present in seeds of milk thistle plant (Silybum marianum). The current study investigated pathological effects of cadmium (Cd) and their amelioration with silymarin (SL) and milk thistle (MT) quails. A total of 144 quails were equally divided into 9 groups and given different combinations of cadmium chloride (150 and 300 mg/kg feed), SL (250 mg/kg), and MT (10 g/kg) feed. Parameters studied were clinical signs, mortality, organ weights, testes weight and volume, and gross and microscopic pathology. Results of this study indicated an increased mortality and reduced body weight in cadmium-treated quails. Quails were dull, depressed compared with control. Swollen hemorrhagic liver along with atrophied testes were also observed in these groups. No active spermatozoa were observed in lumen of seminiferous tubules of Cd-treated birds presenting arrest of spermatogenesis. Supplementing MT and SL ameliorated mortality, organ weights, spermatogenesis, and histopathological lesions. It may be concluded that MT and SL proved beneficial in cadmium-induced toxicities in Japanese quails.

ACS Style

Muhammad Kashif Saleemi; Muhammad Waseem Tahir; Rao Zahid Abbas; Masood Akhtar; Aamir Ali; Muhammad Tariq Javed; Zahida Fatima; Muhammad Zubair; Sheraz Ahmed Bhatti; Zahoor Ul Hassan. Amelioration of toxicopathological effects of cadmium with silymarin and milk thistle in male Japanese quail (Coturnix japonica). Environmental Science and Pollution Research 2019, 26, 21371 -21380.

AMA Style

Muhammad Kashif Saleemi, Muhammad Waseem Tahir, Rao Zahid Abbas, Masood Akhtar, Aamir Ali, Muhammad Tariq Javed, Zahida Fatima, Muhammad Zubair, Sheraz Ahmed Bhatti, Zahoor Ul Hassan. Amelioration of toxicopathological effects of cadmium with silymarin and milk thistle in male Japanese quail (Coturnix japonica). Environmental Science and Pollution Research. 2019; 26 (21):21371-21380.

Chicago/Turabian Style

Muhammad Kashif Saleemi; Muhammad Waseem Tahir; Rao Zahid Abbas; Masood Akhtar; Aamir Ali; Muhammad Tariq Javed; Zahida Fatima; Muhammad Zubair; Sheraz Ahmed Bhatti; Zahoor Ul Hassan. 2019. "Amelioration of toxicopathological effects of cadmium with silymarin and milk thistle in male Japanese quail (Coturnix japonica)." Environmental Science and Pollution Research 26, no. 21: 21371-21380.

Journal article
Published: 29 April 2019 in Food Control
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This study aimed to explore the incidence of Fusarium fungi and their mycotoxins in marketed feed in Qatar. Samples of mixed-grain-cereal, maize, wheat, millet, barley, oat and soybean were tested for the presence of zearalenone (ZEN), fumonisins, T2/HT2 and deoxynevalenol (DON) mycotoxins. PCR-based identification of the Fusarium isolates was followed by investigation into genes involved in trichothecenes (tri gene cluster), fumonisins (fum gene cluster) and ZEN (pks gene) biosynthesis. In-vitro mycotoxin production potential of the isolates was compared with their molecular profiles. Mixed-grain cereals showed highest contamination with Fusarium, followed by maize and wheat. The incidence of ZEN was highest in mixed grain samples (87.5%) followed by maize (50%), millet (42.85%) and wheat (40%). In case of DON; 62.5%, 30%, 40%, 14.28%, 50% and 66.67% of the tested mixed cereal grain, maize, wheat, millet, barley and soybean samples were positive. Based on PCR results, F. verticillioides showed highest frequency distribution (34%), followed by F. graminearum (16%), F. oxysporum (15%), F. proliferatum (13%), F. culmorum (8%), F. solani (7%), F. subglutinans (4%) and F. avenaceum (3%) in the feed cereals. All the isolates of F. verticillioides presented both fum1 and fum13 genes, and produced fumonisin mycotoxins in-vitro. All the isolates of F. graminearum and F. culmorum had the tri5 and tri6 genes and resulted in DON accumulation in rice media. In addition to DON, all the F. culmorum isolates produced ZEN in artificially contaminated media, in line with their molecular profile of having tri5, tri6 and pks13 genes. The findings of present study confirmed the occurrence of toxigenic Fusarium and their mycotoxins in animal feed, all below the maximum limit set by EU. Further, the in-vitro mycotoxin production potential of the isolates was in agreement with their tri, fum and pks genetic profiles.

ACS Style

Zahoor Ul Hassan; Roda Al Thani; Virgilio Balmas; Quirico Migheli; Samir Jaoua. Prevalence of Fusarium fungi and their toxins in marketed feed. Food Control 2019, 104, 224 -230.

AMA Style

Zahoor Ul Hassan, Roda Al Thani, Virgilio Balmas, Quirico Migheli, Samir Jaoua. Prevalence of Fusarium fungi and their toxins in marketed feed. Food Control. 2019; 104 ():224-230.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al Thani; Virgilio Balmas; Quirico Migheli; Samir Jaoua. 2019. "Prevalence of Fusarium fungi and their toxins in marketed feed." Food Control 104, no. : 224-230.

Journal article
Published: 01 January 2019 in Kafkas Universitesi Veteriner Fakultesi Dergisi
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ACS Style

Hayatullah Khan; Farhan Anwar Khan; Umar Sadique; Shakoor Ahmad; Zahoor Ul Hassan; Faisal Ahmad; Muqader Shah; Ijaz Ahmad; Zahir Shah; Zia Ur-Rehman. Ot Sazanı (Ctenopharyngodon idella)’nda Aflatoksin B1’in Genotoksik ve Toksikopatolojik Etkileri. Kafkas Universitesi Veteriner Fakultesi Dergisi 2019, 1 .

AMA Style

Hayatullah Khan, Farhan Anwar Khan, Umar Sadique, Shakoor Ahmad, Zahoor Ul Hassan, Faisal Ahmad, Muqader Shah, Ijaz Ahmad, Zahir Shah, Zia Ur-Rehman. Ot Sazanı (Ctenopharyngodon idella)’nda Aflatoksin B1’in Genotoksik ve Toksikopatolojik Etkileri. Kafkas Universitesi Veteriner Fakultesi Dergisi. 2019; ():1.

Chicago/Turabian Style

Hayatullah Khan; Farhan Anwar Khan; Umar Sadique; Shakoor Ahmad; Zahoor Ul Hassan; Faisal Ahmad; Muqader Shah; Ijaz Ahmad; Zahir Shah; Zia Ur-Rehman. 2019. "Ot Sazanı (Ctenopharyngodon idella)’nda Aflatoksin B1’in Genotoksik ve Toksikopatolojik Etkileri." Kafkas Universitesi Veteriner Fakultesi Dergisi , no. : 1.

Journal article
Published: 01 November 2018 in International Journal of Food Microbiology
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Many foods and beverages in temperate and tropical regions are prone to contamination by ochratoxin A (OTA), one of the most harmful mycotoxins for human and animal health. Aspergillus ochraceus and Aspergillus carbonarius are considered among the main responsible for OTA contamination. We have previously demonstrated that four low or non- fermenting yeasts are able to control the growth and sporulation of OTA-producing Aspergilli both in vitro and on detached grape berries: the biocontrol effect was partly due to the release of volatile organic compounds (VOCs). Aiming to further characterise the effect of VOCs produced by biocontrol yeast strains, we observed that, beside vegetative growth and sporulation, the volatile compounds significantly reduced the production of OTA by two A. carbonarius and A. ochraceus isolates. Exposure to yeast VOCs also affected gene expression in both species, as confirmed by downregulation of polyketide synthase, non-ribosomal peptide synthase, monooxygenase, and the regulatory genes laeA and veA. The main compound of yeast VOCs was 2-phenylethanol, as detected by Headspace-Solid Phase Microextraction-Gas Chromatography-Tandem Mass Spectrometry (HS-SPME-GC–MS) analysis. Yeast VOCs represent a promising tool for the containment of growth and development of mycotoxigenic fungi, and a valuable aid to guarantee food safety and quality.

ACS Style

Maria Grazia Farbo; Pietro Paolo Urgeghe; Stefano Fiori; Angela Marcello; Stefania Oggiano; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. Effect of yeast volatile organic compounds on ochratoxin A-producing Aspergillus carbonarius and A. ochraceus. International Journal of Food Microbiology 2018, 284, 1 -10.

AMA Style

Maria Grazia Farbo, Pietro Paolo Urgeghe, Stefano Fiori, Angela Marcello, Stefania Oggiano, Virgilio Balmas, Zahoor Ul Hassan, Samir Jaoua, Quirico Migheli. Effect of yeast volatile organic compounds on ochratoxin A-producing Aspergillus carbonarius and A. ochraceus. International Journal of Food Microbiology. 2018; 284 ():1-10.

Chicago/Turabian Style

Maria Grazia Farbo; Pietro Paolo Urgeghe; Stefano Fiori; Angela Marcello; Stefania Oggiano; Virgilio Balmas; Zahoor Ul Hassan; Samir Jaoua; Quirico Migheli. 2018. "Effect of yeast volatile organic compounds on ochratoxin A-producing Aspergillus carbonarius and A. ochraceus." International Journal of Food Microbiology 284, no. : 1-10.

Journal article
Published: 01 October 2018 in Food Control
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This study was designed to explore the incidence of aflatoxin M1 (AFM1) in milk and dairy products marketed in Qatar. Milk (n=72), yogurt (n=21), cheese (n=46), butter (n=18) and laban (n=25) samples were initially screened by competitive enzyme linked immunosorbent assay (ELISA) for the presence of AFM1, followed by confirmation with ultra-high-performance liquid chromatography (UHPLC). AFM1 was detected in 85%, 76%, 85%, 67% and 76% of the milk, yogurt, cheese, butter and laban samples, respectively. The levels of AFM1 in pasteurized vs ultra-high temperature (UHT) milk and full- vs low-fat yogurts showed a non-significant difference. Halloumi and Kashkaval cheese varieties showed a significantly higher AFM1 contents as compared to Mozzarella, Edam, Cheddar, cream and Moshalal cheese. Likewise, unsalted butter demonstrated significantly (p<0.05) higher AFM1 values than salted butter samples. None of the tested samples presented AFM1 levels above the EU maximum limits of 50 ng/L or kg for milk, yogurt and butter, and 250 ng/kg for cheese. From the finding of present study, it can be concluded that, although high percentage of dairy products marketed in Qatar demonstrated AFM1 contents, but do not represent a public health concern considering the EU maximum limits.

ACS Style

Zahoor Ul Hassan; Roda Al-Thani; Fathy A. Atia; Saeed Almeer; Virgilio Balmas; Quirico Migheli; Samir Jaoua. Evidence of low levels of aflatoxin M1 in milk and dairy products marketed in Qatar. Food Control 2018, 92, 25 -29.

AMA Style

Zahoor Ul Hassan, Roda Al-Thani, Fathy A. Atia, Saeed Almeer, Virgilio Balmas, Quirico Migheli, Samir Jaoua. Evidence of low levels of aflatoxin M1 in milk and dairy products marketed in Qatar. Food Control. 2018; 92 ():25-29.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al-Thani; Fathy A. Atia; Saeed Almeer; Virgilio Balmas; Quirico Migheli; Samir Jaoua. 2018. "Evidence of low levels of aflatoxin M1 in milk and dairy products marketed in Qatar." Food Control 92, no. : 25-29.

Journal article
Published: 01 September 2018 in pakistan journal of pharmaceutical sciences
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ACS Style

Muhammad Avais; Muhammad Sarwar Khan; Muhammad Arif Khan; Kamran Ashraf; Zahoor Ul Hassan; Sajid Hameed; Jawaria Ali Khan. Histopathological changes induced in liver, kidney, heart and pancreas of rabbits by prolonged oral cyanide exposure. pakistan journal of pharmaceutical sciences 2018, 31, 1797 -1803.

AMA Style

Muhammad Avais, Muhammad Sarwar Khan, Muhammad Arif Khan, Kamran Ashraf, Zahoor Ul Hassan, Sajid Hameed, Jawaria Ali Khan. Histopathological changes induced in liver, kidney, heart and pancreas of rabbits by prolonged oral cyanide exposure. pakistan journal of pharmaceutical sciences. 2018; 31 (5):1797-1803.

Chicago/Turabian Style

Muhammad Avais; Muhammad Sarwar Khan; Muhammad Arif Khan; Kamran Ashraf; Zahoor Ul Hassan; Sajid Hameed; Jawaria Ali Khan. 2018. "Histopathological changes induced in liver, kidney, heart and pancreas of rabbits by prolonged oral cyanide exposure." pakistan journal of pharmaceutical sciences 31, no. 5: 1797-1803.

Journal article
Published: 29 August 2018 in Toxicon
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This study aimed to evaluate the effect of dietary ochratoxin A (OA), in the presence and absence of L-carnitine (LC) and vitamin E (VE), on the humoral immune responses of White Leghorn cockerels (WLC). One-day old white male Leghorn chicks were divided into 12 groups, having 20 birds each and were offered ration contaminated with OA (1.0 or 2.0 mg/kg feed) alone and concurrently with LC (1.0 g/kg) and/or VE (0.2 g/kg), for 42 days. The humoral immune responses were accessed by lymphoproliferative response to avian tuberculin, in-vivo phagosomes activity to carbon particles and antibody response to the sheep red blood cells (SRBCs). The dietary addition of OA alone suppressed the humoral immune responses, however, the exposure of birds to 1.0 mg/kg OA in the presence of LC and/or VE showed a significant reduction in OA induced immunotoxicity. This protective response was absent in the birds fed 2.0 mg/kg OA in the presence and absence of LC and/or VE. Histopathological and morphometric examination of the bursa of Fabricius exhibited a decrease in the severity and frequency of OA induced lesions in the presence of dietary LC and/or VE. The use of LC and VE as dietary supplement, can effectively overcome OA (≤1.0 mg/kg) induced immunosuppression.

ACS Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Zahoor Ul Hassan; Muhammad Kashif Saleemi; Aisha Khatoon; Zain Ul Abidin; Muhammad Raza Hameed. Dietary L-carnitine and vitamin-E; a strategy to combat ochratoxin-A induced immunosuppression. Toxicon 2018, 153, 62 -71.

AMA Style

Sheraz Ahmed Bhatti, Muhammad Zargham Khan, Zahoor Ul Hassan, Muhammad Kashif Saleemi, Aisha Khatoon, Zain Ul Abidin, Muhammad Raza Hameed. Dietary L-carnitine and vitamin-E; a strategy to combat ochratoxin-A induced immunosuppression. Toxicon. 2018; 153 ():62-71.

Chicago/Turabian Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Zahoor Ul Hassan; Muhammad Kashif Saleemi; Aisha Khatoon; Zain Ul Abidin; Muhammad Raza Hameed. 2018. "Dietary L-carnitine and vitamin-E; a strategy to combat ochratoxin-A induced immunosuppression." Toxicon 153, no. : 62-71.

Journal article
Published: 14 June 2018 in Toxins
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Mycotoxins are important contaminants of food and feed. In this study, low fermenting yeast (Lachancea thermotolerans) and its derivatives were applied against toxigenic fungi and their mycotoxins. A. parasiticus, P. verrucosum and F. graminearum and their mycotoxins were exposed to yeast volatile organic compounds (VOCs) and cells, respectively. VOCs reduced significantly the fungal growth (up to 48%) and the sporulation and mycotoxin synthesis (up to 96%). Very interestingly, it was shown that even 7 yeast colonies reduced Fusarium’s growth and the synthesis of its mycotoxin, deoxynivalenol (DON). Moreover, decreasing yeast nutrient concentrations did not affect the inhibition of fungal growth, but reduced DON synthesis. In addition, inactivated yeast cells were able to remove up to 82% of the ochratoxin A (OTA). As an application of these findings, the potentialities of the VOCs to protect tomatoes inoculated with F. oxysporum was explored and showed that while in the presence of VOCs, no growth was observed of F. oxysporum on the inoculated surface areas of tomatoes, in the absence of VOCs, F. oxysporum infection reached up to 76% of the tomatoes’ surface areas. These results demonstrate that the application of yeasts and their derivatives in the agriculture and food industry might be considered as a very promising and safe biocontrol approach for food contamination.

ACS Style

Randa Zeidan; Zahoor Ul Hassan; Roda Al-Thani; Virgilio Balmas; Samir Jaoua. Application of Low-Fermenting Yeast Lachancea thermotolerans for the Control of Toxigenic Fungi Aspergillus parasiticus, Penicillium verrucosum and Fusarium graminearum and Their Mycotoxins. Toxins 2018, 10, 242 .

AMA Style

Randa Zeidan, Zahoor Ul Hassan, Roda Al-Thani, Virgilio Balmas, Samir Jaoua. Application of Low-Fermenting Yeast Lachancea thermotolerans for the Control of Toxigenic Fungi Aspergillus parasiticus, Penicillium verrucosum and Fusarium graminearum and Their Mycotoxins. Toxins. 2018; 10 (6):242.

Chicago/Turabian Style

Randa Zeidan; Zahoor Ul Hassan; Roda Al-Thani; Virgilio Balmas; Samir Jaoua. 2018. "Application of Low-Fermenting Yeast Lachancea thermotolerans for the Control of Toxigenic Fungi Aspergillus parasiticus, Penicillium verrucosum and Fusarium graminearum and Their Mycotoxins." Toxins 10, no. 6: 242.

Original articles
Published: 15 May 2018 in Food Additives & Contaminants: Part B
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The present study was conducted to explore the occurrence of mycotoxins in commercial baby foods in Doha-Qatar. LCMS/MS- and HPLC-based analysis of baby food (n = 67) for 12 mycotoxins confirmed the presence of aflatoxin M1 (AFM1, 33%), ochratoxin A (OTA, 31%), deoxynivalenol (DON, 27%), aflatoxin B1 (AFB1, 22%), fumonisin B2 (FB2, 10%), zearalenone (ZEN, 4%) and T-2 toxin (2%). Noodles exhibited the maximum contamination percentage, with 33% of the samples being contaminated above the EU maximum limits, for at least one mycotoxin. Among the multi-grain flake samples, up to 28% and for the milk and milk-based-cereal samples, 14% contained at least one mycotoxin above the EU maximum limits. From all cereal-based food samples, 22%, 5%, 2% and 2% were concurrently contaminated with 2, 3, 4 and 5 mycotoxins, respectively. The occurrence of toxicological important mycotoxins in Qatari market warrants the implementation of strict regulatory limits to protect human health.

ACS Style

Zahoor Ul Hassan; Roda Al Thani; Fathy A. Atia; Saeed Al Meer; Quirico Migheli; Samir Jaoua. Co-occurrence of mycotoxins in commercial formula milk and cereal-based baby food on the Qatar market. Food Additives & Contaminants: Part B 2018, 11, 191 -197.

AMA Style

Zahoor Ul Hassan, Roda Al Thani, Fathy A. Atia, Saeed Al Meer, Quirico Migheli, Samir Jaoua. Co-occurrence of mycotoxins in commercial formula milk and cereal-based baby food on the Qatar market. Food Additives & Contaminants: Part B. 2018; 11 (3):191-197.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Al Thani; Fathy A. Atia; Saeed Al Meer; Quirico Migheli; Samir Jaoua. 2018. "Co-occurrence of mycotoxins in commercial formula milk and cereal-based baby food on the Qatar market." Food Additives & Contaminants: Part B 11, no. 3: 191-197.

Journal article
Published: 01 March 2018 in pakistan journal of pharmaceutical sciences
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ACS Style

Muhammad Avais; Muhammad Sarwar Khan; Muhammad Arif Khan; Kamran Ashraf; Zahoor Ul Hassan; Jawaria Ali Khan; Nisar Ahmad. Assessing the efficacy of aqueous garlic extract, sodium nitrite and sodium thiosulfate against prolonged oral cyanide exposure in rabbits. pakistan journal of pharmaceutical sciences 2018, 31, 411 -419.

AMA Style

Muhammad Avais, Muhammad Sarwar Khan, Muhammad Arif Khan, Kamran Ashraf, Zahoor Ul Hassan, Jawaria Ali Khan, Nisar Ahmad. Assessing the efficacy of aqueous garlic extract, sodium nitrite and sodium thiosulfate against prolonged oral cyanide exposure in rabbits. pakistan journal of pharmaceutical sciences. 2018; 31 (2):411-419.

Chicago/Turabian Style

Muhammad Avais; Muhammad Sarwar Khan; Muhammad Arif Khan; Kamran Ashraf; Zahoor Ul Hassan; Jawaria Ali Khan; Nisar Ahmad. 2018. "Assessing the efficacy of aqueous garlic extract, sodium nitrite and sodium thiosulfate against prolonged oral cyanide exposure in rabbits." pakistan journal of pharmaceutical sciences 31, no. 2: 411-419.

Journal article
Published: 01 February 2018 in Food Control
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Zahoor Ul Hassan; Roda Fahad Al-Thani; Quirico Migheli; Samir Jaoua. Detection of toxigenic mycobiota and mycotoxins in cereal feed market. Food Control 2018, 84, 389 -394.

AMA Style

Zahoor Ul Hassan, Roda Fahad Al-Thani, Quirico Migheli, Samir Jaoua. Detection of toxigenic mycobiota and mycotoxins in cereal feed market. Food Control. 2018; 84 ():389-394.

Chicago/Turabian Style

Zahoor Ul Hassan; Roda Fahad Al-Thani; Quirico Migheli; Samir Jaoua. 2018. "Detection of toxigenic mycobiota and mycotoxins in cereal feed market." Food Control 84, no. : 389-394.

Comparative study
Published: 16 August 2017 in Journal of the Science of Food and Agriculture
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Mycotoxins contamination in animal products and by-products is a persistent threat to the food and feed industry. The present study was designed to evaluate the comparative inhibitory effects of Bentonite (BN), activated charcoal (AC) and a newly discovered yeast, Trichosporon mycotoxinivorans (TM), against feed-to-tissue transfer of mycotoxins. A dose dependent increase as determined by HPLC, in the residues of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was exhibited in the groups of birds fed AFB1 and OTA alone. The dietary addition of BN and AC to AFB1-contaminated diets resulted in a 41–87% and 16–72% decrease in AFB1 residues in liver of the birds, respectively. However, this decrease was non-significant with addition of TM as AFB1 binder. A partial to non-significant protection was observed by dietary BN and AC, against OTA residues, while a significant decrease in OTA residues (38–84%) was noted in TM-OTA co-fed groups. The order of efficacy in terms of lowering AFB1 residues in the liver was BN > AC > TM, while against OTA it was TM > BN > AC. The findings of present study suggest that, based upon the nature of target mycotoxins, a mixture of multi-mycotoxins binders/detoxifiers should be incorporated in the animal feeds. © 2017 Society of Chemical Industry

ACS Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Zahoor Ul Hassan; Muhammad Kashif Saleemi; Muhammad Saqib; Aisha Khatoon; Masood Akhtar. Comparative efficacy of Bentonite clay, activated charcoal andTrichosporon mycotoxinivoransin regulating the feed-to-tissue transfer of mycotoxins. Journal of the Science of Food and Agriculture 2017, 98, 884 -890.

AMA Style

Sheraz Ahmed Bhatti, Muhammad Zargham Khan, Zahoor Ul Hassan, Muhammad Kashif Saleemi, Muhammad Saqib, Aisha Khatoon, Masood Akhtar. Comparative efficacy of Bentonite clay, activated charcoal andTrichosporon mycotoxinivoransin regulating the feed-to-tissue transfer of mycotoxins. Journal of the Science of Food and Agriculture. 2017; 98 (3):884-890.

Chicago/Turabian Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Zahoor Ul Hassan; Muhammad Kashif Saleemi; Muhammad Saqib; Aisha Khatoon; Masood Akhtar. 2017. "Comparative efficacy of Bentonite clay, activated charcoal andTrichosporon mycotoxinivoransin regulating the feed-to-tissue transfer of mycotoxins." Journal of the Science of Food and Agriculture 98, no. 3: 884-890.

Journal article
Published: 21 March 2017 in Toxin Reviews
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Muhammad Raza Hameed; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Ahrar Khan; Masood Akhtar; Zahoor-Ul- Hassan; Zahid Hussain. Study of ochratoxin A (OTA)-induced oxidative stress markers in broiler chicks. Toxin Reviews 2017, 36, 270 -274.

AMA Style

Muhammad Raza Hameed, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Ahrar Khan, Masood Akhtar, Zahoor-Ul- Hassan, Zahid Hussain. Study of ochratoxin A (OTA)-induced oxidative stress markers in broiler chicks. Toxin Reviews. 2017; 36 (4):270-274.

Chicago/Turabian Style

Muhammad Raza Hameed; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Ahrar Khan; Masood Akhtar; Zahoor-Ul- Hassan; Zahid Hussain. 2017. "Study of ochratoxin A (OTA)-induced oxidative stress markers in broiler chicks." Toxin Reviews 36, no. 4: 270-274.

Research article
Published: 01 January 2017 in Journal of Immunotoxicology
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The present study was designed to investigate any ameliorative effects of bentonite (BN) against immuno-pathological alterations induced by dietary aflatoxin B1 (AFB1) or ochratoxin A (OTA) in broiler chicks. In one experiment, AFB1 (0.1, 0.2 or 0.6 mg/kg feed) was fed alone and par alley with bentonite clay (3.7 or 7.5 g/kg feed) to the broilers. In the second experiment, the broilers were given feed contaminated with OTA (0.15, 0.3 or 1.0 mg/kg feed) alone and in combination with bentonite clay (3.7, 7.5, or 15 g/kg feed). Experimental feedings were continued for 42 days. At various time points along the feeding schedule, immune system organ histologic status, as well as host humoral and cellular immune responses, were evaluated in all groups. The dietary addition of AFB1 and OTA alone significantly reduced immune responses in the birds as assessed by histological changes in the bursa of Fabricius and thymus, antibody responses to SRBC, in-vivo lympho-proliferative responses to Phytohemagglutinin-P (PHA-P) and, phagocytic function in situ. The dietary addition of BN significantly ameliorated the immunotoxicity of 0.1 and 0.2 mg/kg dietary AFB1, however with a level of 0.6 mg AFB1/kg only partial amelioration was seen. The co-treatment of birds exposed to OTA with BN at all levels only partially alleviated deleterious effects on histology and immune responses. Taken together, the results here suggested to us that dietary addition of BN could help ameliorate AFB1-mediated immunotoxicities but could not afford such protection against OTA-induced immune damage.

ACS Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Muhammad Saqib; Ahrar Khan; Zahoor Ul-Hassan. Protective role of bentonite against aflatoxin B1- and ochratoxin A-induced immunotoxicity in broilers. Journal of Immunotoxicology 2017, 14, 66 -76.

AMA Style

Sheraz Ahmed Bhatti, Muhammad Zargham Khan, Muhammad Kashif Saleemi, Muhammad Saqib, Ahrar Khan, Zahoor Ul-Hassan. Protective role of bentonite against aflatoxin B1- and ochratoxin A-induced immunotoxicity in broilers. Journal of Immunotoxicology. 2017; 14 (1):66-76.

Chicago/Turabian Style

Sheraz Ahmed Bhatti; Muhammad Zargham Khan; Muhammad Kashif Saleemi; Muhammad Saqib; Ahrar Khan; Zahoor Ul-Hassan. 2017. "Protective role of bentonite against aflatoxin B1- and ochratoxin A-induced immunotoxicity in broilers." Journal of Immunotoxicology 14, no. 1: 66-76.