Appropriate interventions to improve working equine welfare should be proposed according to scientific evidence that arises from different geo-cultural contexts. This study aims to assess and compare the welfare status of working horses in two administrative regions of Chile and to determine how owners perceive their horses. Horses’ welfare status was assessed through direct indicators (direct observation and clinical examination) and indirect indicators (an interview with the owner). Owners′ perceptions of their horses were determined through a discourse analysis of their statements. In total, 100 horses and 100 owners were assessed. Results showed a low prevalence of health problems and negative behavior responses among horses in the two regions evaluated. Significant associations were found between inadequate body condition and the absence of deworming, and between hoof abnormalities and a low frequency of shoeing. Between regions, significant differences were found in the presence of lesions and the person responsible for horseshoeing. In regards to the owners′ appreciations, two differing perceptions of working horses were found: a predominantly affective perception and a perception of the animal as a working instrument. Although the instrumental perception was more frequent in the Araucania region, the affective perception was widely shared by both owner populations. The results reveal a good welfare status in working horses and suggest that both affective and instrumental perceptions of these animals can coexist.

Mules are essential for pack work in mountainous areas, but there is a lack of research on this species. This study intends to assess the perceptions, attitudes, empathy and pain perception of soldiers about mules, to understand the type of human–mule relationship. For this, a survey was applied with closed-ended questions where the empathy and pain perception tools were included and later analyzed through correlations. Open-ended questions were analyzed through text mining. A total of 73 soldiers were surveyed. They had a wide range of ages and years of experience working with equids. Significant positive correlations were found between human empathy, animal empathy and pain perception. Soldiers show a preference for working with mules over donkeys and horses. Text mining analysis shows three clusters associated with the mules’ nutritional, environmental and health needs. In the same line, relevant relations were found for the word “attention” with “load”, “food”, and “harness”. When asked what mules signify for them, two clusters were found, associated with mules’ working capacity and their role in the army. Relevant relations were found between the terms “mountain”, “support”, and “logistics”, and also between “intelligent” and “noble”. To secure mules’ behavioral and emotional needs, future training strategies should include behavior and welfare concepts.

Understanding how children and adolescents perceive zoo animal welfare provides insights into public views on captive conditions. This study used a mixed-methods approach to investigate these perceptions. A total of 254 participants (aged 7–18) were surveyed, with 113 completing the full circuit, which included species from distinct phylogenetic groups: monkey, macaw, caiman, frog, fish, and tarantula. Qualitative analysis (n = 254) evaluated children’s recognition of animal needs, whereas quantitative analysis (n = 113) examined how phylogenetic distance, participants’ emotional state, and overall welfare perception influenced animal and environmental assessments—a structured survey evaluating perceived animal health, enclosure conditions, and behavioral expression. Participants’ understanding of animal welfare extended beyond basic needs, recognizing the importance of species-specific behaviors and appropriate environments. Environmental and animal assessment scores were significantly influenced by species, participants’ emotional states, and overall welfare perceptions: fish received the highest scores, positive emotions were associated with higher ratings, and a better overall welfare perception correlated with more favorable assessments. We inferred that phylogenetic distance, emotional state, and general welfare impressions shaped how children and adolescents evaluate animal welfare. The study supported zoo-based educational strategies and reinforced the role of zoos in promoting welfare awareness and conservation-oriented attitudes.