The saliva of athletes participating in anaerobic (track and field athletes) or aerobic (swimmers) physical activity was studied. Saliva sample was collected before (control) and after the workout. It was found that physical activity significantly increased the salivary total protein concentration and pH values in the athletes. The activity of catalase in saliva was significantly increased only after aerobic, but not anaerobic exercise performance. The bioluminescent indicator of athletes’ saliva is significantly higher during aerobic workout than anaerobic exercise. Salivary biomarkers such as lactate concentration, catalase activity, total protein concentration, calcium, potassium, magnesium ions, and the amide IV group may have a significant effect on the enhancement of light emission in a bioluminescent assay based on a system of coupled enzymatic reaction. The amide IV group and the presence of the salivary oligosaccharides, polysaccharides, phosphatase, and phospholipids in athletes during anaerobic physical activity can eliminate bioluminescence more efficiently than the concentration of triene conjugates and the amide II group in the saliva of athletes involved in aerobic exercises. Thus, significant biomarkers for the measurement of the physiological status of athletes who engaged in aerobic or anaerobic physical activity have been identified in saliva, these biomarkers can influence the integrated response of the bioluminescent enzymatic biotest. The change in the integrated bioluminescent indicator of athletes' saliva depending on the type of physical exertion can be used in sports medicine to prevent physical overload.

This paper proposes bioluminescent enzymatic assay of saliva as a non-invasive method for monitoring the functional state of the body. The value of luminescence intensity from coupled enzyme-based reaction catalyzed by NADH:FMN oxidoreductase and luciferase, when exposed to saliva, served as an indicator of the state of the body of workers at work. The absence of a significant difference between profiles of bioluminescence before and after the shift and no changes in biochemical, physical and chemical parameters of saliva indicated that the bodies had adapted to a workload. An increased bioluminescent intensity value is typical for workers in a state of chronic fatigue, a decreased one points to the presence of chronic diseases and bad habits. The dependence of the bioluminescent intensity value on the concentration of lactate, lipid peroxidation products, ionic and mineral composition, the values of free radical oxidation and antiradical protection was identified. Thus, the dependence of the saliva constituents on conditions of life and health status of workers can be identified using bioluminescent enzyme bioassay that is suitable for rapid monitoring of the body at work.

The paper considers the creation of a diagnostic system based on morphological and biophysical indicators that allows us to understand the influence of the climatic component, as well as stress factors on the general physiological state of young people for further correction of preventive measures and preservation of the health of the peoples of the Far North. The aim of the study is a comprehensive assessment of the health of young people living in the Far North based on the results of bioluminescent saliva activity, stress-emotional state obtained before and after intense mental stress, as well as anthropometric and bioimpedance indicators. 117 students of the Medical Institute of Surgut State University took part in the survey at a young age (18-23 years) during the educational process (rest) and during the examination session (mental load) in the first half of the year of study (autumn-winter 2021). The stress-emotional state was assessed using the Spielberger-Khanin test, the functional state of students They were studied using bioluminescent salivary fluid testing, anthropometric and bioimpedance methods. Samples were obtained by spitting unstimulated saliva into a test tube. As a result of the study, regional features of the physique of young people were revealed, which indicates the formation of a phenotype adapted to living conditions in the northern region. The relationship between the stress-emotional status of adolescents and the level of inhibition of the bioluminescent enzymatic system by saliva samples taken before and after mental stress was shown. The value of the residual glow had an increased indicator for the state of rest and significantly reduced the intensity of the bioluminescent glow under mental stress. The methodological development obtained in the future will make it possible to effectively manage the methods and methods of diagnosing young people, taking into account the influence of external factors, climatic zones and conditions.

Bioluminescent testing of saliva of sports horses sampled before and after physical exercise was carried out using a bioenzyme reaction catalyzed by NADH:FMN-oxidoreductase and bacterial luciferase. The inhibitory effect of saliva on the intensity of the glow was shown, which depended on an increase in the pH of saliva after muscle work. A decrease in the integral bioluminescent indicator during low-intensity muscle work correlated with the content of total protein and glucose in the blood serum. An increase in the bioluminescent indicator at high intensity was due to a decrease in creatinine or an increase in aspartate aminotransferase and is interrelated with an increase in heart rate. It is shown for the first time that the inhibition of bioluminescent glow can be an indicator of the functional state of a horse in training, which can be used in sports horse breeding to prevent overloads.