Mingliang Jiang

Prof. Dr. Mingliang Jiang

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Academic Career Length

11Years (2016-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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28 October 2016 in Journal of Microbiology and Biotechnology
Verified
Journal Article
Closed access
FZFei-Liang Zhong
Fei-Liang Zhong
RMRui Ma
Rui Ma
MJMingliang Jiang
Mingliang Jiang
WDWei-Wei Dong
Wei-Wei Dong
JJJun Jiang
Jun Jiang
LQLin-Hu Quan
Lin-Hu Quan
The ginsenoside-hydrolyzing β-glucosidase gene (bgy2) was cloned from Lactobacillus brevis. We expressed this gene in Escherichia coli BL21(DE3), isolated the resulting protein, and then utilized the enzyme for the biotransformation of ginsenosides. The bgy2 gene contains 2,223 bp, and encodes a protein of 741 amino acids that is a member of glycosyl hydrolase family 3. β-Glucosidase (Bgy2) cleaved the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. Under optimal conditions (pH 7.0, 30°C), we used 0.1 mg/ml Bgy2 in 20 mM sodium phosphate buffer (PBS) for enzymatic studies. In these conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 were converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. In pharmaceutical and commercial industries, this recombinant Bgy2 would be suitable for producting ginsenoside Rd and compound K.
8 July 2022 in Frontiers in Plant Science
Verified
Journal Article
Open access
MJMingliang Jiang
Mingliang Jiang
XLXiaonan Li
Xiaonan Li
XDXiangshu Dong
Xiangshu Dong
YZYe Zu
Ye Zu
ZZZongxiang Zhan
Zongxiang Zhan
HLHong Lang
Hong Lang
Orphan genes (OGs) are defined as genes having no sequence similarity with genes present in other lineages. OGs have been regarded to play a key role in the development of lineage-specific adaptations and can also serve as a constant source of evolutionary novelty. These genes have often been found related to various stress responses, species-specific traits, special expression regulation, and also participate in primary substance metabolism. The advancement in sequencing tools and genome analysis methods has made the identification and characterization of OGs comparatively easier. In the study of OG functions in plants, significant progress has been made. We review recent advances in the fast evolving characteristics, expression modulation, and functional analysis of OGs with a focus on their role in plant biology. We also emphasize current challenges, adoptable strategies and discuss possible future directions of functional study of OGs.
1 August 2016 in Acta Physiologiae Plantarum
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Journal Article
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MJMingliang Jiang
Mingliang Jiang
JLJia Liu
Jia Liu
XQXueli Quan
Xueli Quan
LQLin-Hu Quan
Lin-Hu Quan
SWSongquan Wu
Songquan Wu
1 November 2020 in Horticulture Research
Verified
Journal Article
Open access
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MJMingliang Jiang
Mingliang Jiang
ZZZongxiang Zhan
Zongxiang Zhan
HLHaiyan Li
Haiyan Li
XDXiangshu Dong
Xiangshu Dong
FCFeng Cheng
Feng Cheng
ZPZhongyun Piao
Zhongyun Piao
Orphan genes (OGs), which are genes unique to a specific taxon, play a vital role in primary metabolism. However, little is known about the functional significance of Brassica rapa OGs (BrOGs) that were identified in our previous study. To study their biological functions, we developed a BrOG overexpression (BrOGOE) mutant library of 43 genes in Arabidopsis thaliana and assessed the phenotypic variation of the plants. We found that 19 of the 43 BrOGOE mutants displayed a mutant phenotype and 42 showed a variable soluble sugar content. One mutant, BrOG1OE, with significantly elevated fructose, glucose, and total sugar contents but a reduced sucrose content, was selected for in-depth analysis. BrOG1OE showed reduced expression and activity of the Arabidopsis sucrose synthase gene (AtSUS); however, the activity of invertase was unchanged. In contrast, silencing of two copies of BrOG1 in B. rapa, BraA08002322 (BrOG1A) and BraSca000221 (BrOG1B), by the use of an efficient CRISPR/Cas9 system of Chinese cabbage (B. rapa ssp. campestris) resulted in decreased fructose, glucose, and total soluble sugar contents because of the upregulation of BrSUS1b, BrSUS3, and, specifically, the BrSUS5 gene in the edited BrOG1 transgenic line. In addition, we observed increased sucrose content and SUS activity in the BrOG1 mutants, with the activity of invertase remaining unchanged. Thus, BrOG1 probably affected soluble sugar metabolism in a SUS-dependent manner. This is the first report investigating the function of BrOGs with respect to soluble sugar metabolism and reinforced the idea that OGs are a valuable resource for nutrient metabolism.
16 July 2018 in International Journal of Molecular Sciences
Verified
Journal Article
Open access
PDF file
MJMingliang Jiang
Mingliang Jiang
XDXiangshu Dong
Xiangshu Dong
HLHong Lang
Hong Lang
WPWenxing Pang
Wenxing Pang
ZZZongxiang Zhan
Zongxiang Zhan
ZPZhongyun Piao
Zhongyun Piao
Orphan genes, also called lineage-specific genes (LSGs), are important for responses to biotic and abiotic stresses, and are associated with lineage-specific structures and biological functions. To date, there have been no studies investigating gene number, gene features, or gene expression patterns of orphan genes in Brassica rapa. In this study, 1540 Brassica-specific genes (BSGs) and 1824 Cruciferae-specific genes (CSGs) were identified based on the genome of Brassica rapa. The genic features analysis indicated that BSGs and CSGs possessed a lower percentage of multi-exon genes, higher GC content, and shorter gene length than evolutionary-conserved genes (ECGs). In addition, five types of BSGs were obtained and 145 out of 529 real A subgenome-specific BSGs were verified by PCR in 51 species. In silico and semi-qPCR, gene expression analysis of BSGs suggested that BSGs are expressed in various tissue and can be induced by Plasmodiophora brassicae. Moreover, an A/C subgenome-specific BSG, BSGs1, was specifically expressed during the heading stage, indicating that the gene might be associated with leafy head formation. Our results provide valuable biological information for studying the molecular function of BSGs for Brassica-specific phenotypes and biotic stress in B. rapa.
13 March 2025 in Plant, Cell & Environment
Verified
Journal Article
Closed access
YZYuting Zhang
Yuting Zhang
MJMingliang Jiang
Mingliang Jiang
JMJunjie Ma
Junjie Ma
JCJingjing Chen
Jingjing Chen
LKLiyan Kong
Liyan Kong
ZPZhongyun Piao
Zhongyun Piao