The synthesis of bioplastics from renewable resources is essential for green living. PHBV (poly(3-hydroxybutyrate-co-3-hydroxyvalerate)) is a biodegradable and biocompatible material ideal for various industrial applications. The impact of levulinic (LA), valeric acids (VA), and sodium propionate (SPr) as co-substrates in biomass and the synthesis of 3-hydroxy valerate (3HV) and co-polymerization of PHBV by Burkholderia thailandensis E264 (BtE264) was assessed. Thermogravimetric, XRD, NMR, and mechanical characterization were performed on the homopolymer (PHB) and co-polymer (PHBV), and compared to the PHBV-STD. BtE264 produced the co-polymer PHBV when adding any of the three co-substrates. LA showed a higher positive effect on microbial growth (8.4 g∙L⁻¹) and PHBV production (3.91 g∙L⁻¹), representing 78 and 22 mol % of 3HB and 3HV, respectively. The PHBV obtained with LA had a melting temperature (Tm) lower than the PHB homopolymer and presented lower values for melting enthalpies (ΔHf); the degree of crystallization and TGA values indicated that PHBV had better thermal stability. Additionally, FTIR and NMR revealed that BtE264 synthesizes PHBV with an organization in monomeric units (3HB-3HV), suggesting differentiated incorporation of the monomers, improving 3.4 times the break elongation the co-polymer’s tensile properties. This study highlights the co-substrates’ relevance in PHBV synthesis using BtE264 for the first time.

El PHBV (poli(3-hidroxibutirato-co-3-hidroxivalerato)) es un biopolímero termoplástico que pertenece a la familia de los polihidroxialcanoatos (PHA´s). Este material ha despertado un gran interés d...