Echeveria subrigida is native to Mexico and its methanol extract (ME) shows relevant biological activities for human health, including the α-glucosidase inhibitory (αGI) activity that suggests its antidiabetic potential. Fractionation of the ME based on the αGI activity (IC₅₀ in µg/mL) showed that quercetin-3-O-β-glucoside (131.1), isorhamnetin-3-O-β-glucoside (166.4), and dimers to heptamers proanthocyanidins (9.6) were among the main responsible of αGI activity in the ME. The purified compounds showed better activity than acarbose (IC₅₀ = 4426 µg/mL). Graphical Abstract

The agricultural drainage water from the Sinaloa valley (Mexico) is often reused in agriculture and aquaculture before reaching coastal water bodies. Discharged water must be of good quality to maintain the health of ecosystems and prevent damage to the organisms that compose them. This research determined the occurrence of coliforms in a drainage ditch known as La Michoacana (Sinaloa) that receives contaminated water from agricultural and urban sources. A section of 3.6 km was studied during 2013 and samples were obtained monthly from five equidistant sampling sites. Each water sample was analyzed for total coliform (TC) and fecal coliform (FC) content, pH, salinity, temperature, and dissolved oxygen percentage (%DO). The sampling sites with the highest bacterial contamination were associated with direct discharges of domestic wastewater and those with the lowest to the output drain. The performance of this agricultural ditch decreased the concentration of coliforms, with median and average values of 96% and 87% for TC, and 98% and 85% for FC, respectively. The most common bacteria were Escherichia coli (67.7%), Kluyvera cryocrescens (10.2%), and Enterobacter agglomerans (6.3%). Coliform concentration was positively correlated with temperature and negatively with %DO. The ditch improved the microbiological quality of the water, demonstrating remediation activities in the system. Proper management and maintenance of agricultural drainage ditches would favorably impact the health and biodiversity, as well as the intensive aquaculture and agricultural activities of the Sinaloa valley. El agua que conducen los canales de drenaje del valle agrícola sinaloense (México) es reutilizada con frecuencia en la agricultura y acuacultura antes de ser depositada en las aguas costeras. La calidad del agua descargada debe ser adecuada para mantener la salud de los ecosistemas y evitar daño a los organismos que los componen. Esta investigación determinó la presencia de coliformes en el canal de drenaje agrícola conocido como La Michoacana (Sinaloa), que recibe agua contaminada de fuentes agrícolas y urbanas. Se estudió una sección de 3.6 km del canal durante el año 2013 y se recolectaron mensualmente muestras de agua de cinco puntos de muestreo equidistantes. A cada muestra se le analizó el contenido de coliformes totales (CT) y coliformes fecales (CF), pH, salinidad, temperatura y porcentaje de saturación de oxígeno disuelto (%OD). Los puntos de muestreo con mayor contaminación bacteriana estuvieron asociados a las descargas directas de agua residual doméstica, y aquellos con menor contaminación estuvieron asociados a la salida del canal de drenaje. El funcionamiento del canal disminuyó la carga de coliformes, con medianas y valores promedio del 96% y 87% para CT, y 98% y 85% para CF, respectivamente. Las principales bacterias identificadas fueron Escherichia coli (67.7%), Kluyvera cryocrescens (10.2%) y Enterobacter agglomerans (6.3%). La concentración de coliformes presentó una correlación positiva con la temperatura y negativa con el %OD. El canal de drenaje La Michoacana mejora la calidad microbiológica del agua que conduce, lo cual demuestra funciones de remediación en el sistema. El mantenimiento y manejo adecuado de los canales de drenaje agrícola puede impactar favorablemente la salud y biodiversidad, así como las actividades acuícolas y agrícolas intensivas del valle sinaloense.

In this work, we synthesized a series of nine tetraalkylammonium salts derived from N ‐methyl morpholine and assessed their antibacterial efficacy against Methicillin‐Resistant Staphylococcus aureus (MRSA). The investigated morpholinium cations differ by the length of one linear alkyl chain, which ranges from 1 to 18 carbon atoms. We found that compounds with alkyl chains in the n ‐C ₁₂ H ₂₅ ‐( n ‐dodecyl) to C ₁₆ H ₃₃ ‐( n ‐hexadecyl) display the highest bactericidal effects, exhibiting Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values of 3.9 μg/mL – surpassing the effectiveness of the commercial drug Linezolid. Conversely, compounds with shorter chains (<5 carbon atoms) are inactive against MRSA, establishing a clear structure‐activity relationship. Assays on A. salina reveal that short‐alkyl‐chain morpholinium derivatives, inactive against MRSA), are moderately toxic, while the strongest bactericides of our set demonstrate high or extreme toxicity to A. salina . Our findings underscore the potential of morpholine derivatives as a means to control Methicillin‐Resistant Staphylococcus aureus and pinpoint the need to develop safe applications for these compounds given their potential toxicity.