The experiment was to investigate the effect of different dietary lipid levels on the growth performance, nucleic acids, leptin and adiponectin as well as their receptor gene expression in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Six groups of the juveniles with 40 days of age in triplicate were fed for 90 days using 6 iso‐nitrogen (34% dietary protein) diets. The 6 diets were control diet contained 0.35% of lipid, without lipid supplementation, and the other diets were added different doses of fish oil to make the different dietary lipid levels of 3.35%, 6.35%, 9.35%, 12.35% and 15.35% based on per kg of dried feed. The samples of whole fish and major tissues/organs were randomly taken at 0 and 90 days for determination, respectively. The main results were as follows: compared with the control diet, the diets with different lipid supplementation improved significantly (p < .05) the specific growth rate (SGR), crude fat, nucleic acids, adiponectin receptors 1 and 2 (AdipoRs 1 & 2) gene expression and serum leptin level in juvenile GIFT tilapia. However, the diets with different lipid supplementation reduced significantly (p < .05) the feed conversion ratio (FCR), hepatosomatic index (HSI), crude protein, leptin receptor (LepR) gene expression and serum adiponectin level. There was no significant effect (p > .05) on the survival rate (SR) in the juveniles fed the diets with different lipid supplementation. In conclusion, the diets with different lipid supplementation could affect significantly the SGR, FCR, HIS, nucleic acids, crude fat and protein, leptin and adiponectin levels and their receptor gene expression of the juveniles. The optimal dietary lipid level was 10.52% for SGR and 10.61% for FCR based on the second‐order polynomial regression analysis between dietary lipid levels against the SGR and FCR of the juveniles, respectively, in the experiment.