The objective of this study was to investigate the impacts of Bacillus subtilis (BS), ATCC 6051a strain, as a probiotic bacterium in broiler diets based of 2 protein sources (soybean meal [SBM] and cowpea seeds [CWP]), on growth performance (GP), carcass traits, bone mineralization, and microflora population (0 to 42 d age). The SBM and CWP starter, grower, and finisher diets were tested in the presence or absence of BS (5.0 × 1011 CFU spores g−1 feed) in a 2 × 2 factorial arrangement of treatments in a completely randomized design. Broilers were randomly assigned to 4 dietary treatments with 6 replicate pens per treatment (20 chicks per pen). The results showed that broilers fed CWP had comparable GP (body weight gain, feed intake, and feed conversion ratio) to the birds fed the SBM diet. Carcass, breast and legs' yield, organ size (i.e., gizzard, liver, pancreas, small intestine, cecum), and bone development were not affected by the protein source. The addition of BS in both types of diet improved BWG (P < 0.001) and feed efficiency, especially in the grower and finisher period (P = 0.047; P = 0.043, respectively). In addition, BS significantly decreased abdominal fat (P = 0.026) and cecum weight (P = 0.034) and increased tibia bone P concentration (P = 0.015). Furthermore, BS decrease cecal pH (P = 0.010) and reduced Escherichia coli and Staphylococcus spp. from cecum and excreta broilers (P < 0.001; P < 0.0001, respectively). It is concluded that the BS significantly improved the GP of broilers and can beneficially affect the gut and excreta bacterial community in both SBM and CWP diets.

Bombyx mori is an ideal lepidopteran species representative of many scientific studies, a model of studies for medicine and a significant insect from an ecological standpoint. This review was performed to summarize the fatty acids (FA) composition of silkworm pupae (SP) that are associated with other important compounds that could add value to SP, diversifying the ways of valorization. The proposal to complete plant-based feeds with insect-based feeds represents a viable option to beneficially impact human and animal health and the environment. The quality and quantity of fats consumed significantly impact the aetiology of certain diseases. The key compounds of fat named essential FA (EFA) substantially influence the prevention and treatment of several diseases through their nutraceutical functions. Due to its excellent profile in nutrients such as protein and fat, amino acids and fatty acids composition, SP has become an important alternative feed ingredient and source of EFA. SP is a by-product that was discarded in large quantities. Following the need to act to improve human health and reduce climate change impact, many researchers focused on studying SP applications in the medical and agricultural industries. Several authors noticed an improvement in the health markers by using SP. The feed cost for the animal was reduced with economic implications. Minimization of environmental impact was recorded. Few precautions were recommended regarding SP use, although they should not be ignored. The composition of SP and its potential for use in various industries provides us with persuasive arguments for continuing to develop the sericulture industry.

The main concerns and challenges of raising silkworms include economic value, mulberry management, biodiversity conservation of genetic resources, and developing highly productive breeds for genetic variety. This study investigated the relationship between the economic relevance of the products generated throughout the value chain, limitations, and opportunities to generate incomes for sericulture farmers, trends, and perspectives worldwide, particularly in Romania. Seventy-seven publications were considered from online databases. The diversification of products generated at each level of the value chain of silkworm rearing and their multipurpose applications impact social and economic life. Hence, silk is well known as a valuable biomaterial for industry, suitable for textile and medicine. There are several arguments to use silkworms in human food even though they are not yet authorized as edible insects at the European level. Thus, as a nutrient-rich by-product, silkworm pupae (extract, cakes, and oil) have medicinal properties and can be used for human and animal nutrition. Sericin, silk fibroin, and chitin are bioactive compounds in cocoons and pupae with pharmacological implications and drug composition, while biomass is suitable for biodiesel and excreta for compost. The farmers’ attitudes and mentality associated with political circumstances influence the perspectives for the sericulture field. Due to the high likelihood of using their products, small-medium-scale farmers might benefit sericulture by identifying new sales marketplaces and finding new beneficiaries for directing their multiple products. The funds allotted by government subventions for supporting this fascinating activity and opportunities for jobs may aid in encouraging to start of a new sericulture business or to contribute developing the existing one.

This study shows the antioxidant effect of a dietary hemp seed diet rich in ω-6 polyunsaturated fatty acid (PUFA) on oxidative status in sows during late gestation and lactation and their offspring. Ten pregnant sows were divided into two groups and fed either a control diet (CD) or a hemp diet (HD) containing 2% hemp seed meal for a period of 10 days before farrowing and 5% throughout the lactation period (21 d). After farrowing, 16 of their resulting piglets were divided into two groups: control group CD (eight piglets derived from control sows) and HD group (eight piglets derived from HD sows), respectively. Blood collected from sows and piglets at day 1, 7 and 21 was used for the measurement of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), glutathione (GPx)), nitric oxide production (NO), lipid peroxidation (thiobarbituric acid reactive substances—TBARS), reactive oxygen species (ROS) generation and total antioxidant capacity (TAC) in plasma. The results showed a significant improvement in the oxidative status of sows fed HD throughout lactation compared with CD. Similarly, in piglets, HD positively influenced the activities of antioxidant enzymes, TAC and NO levels and significantly decreased lipid peroxidation in plasma until weaning, in comparison with the CD group. This study suggests the potential of hemp seed diet to improve the overall antioxidant status of the lactating sows and their progeny.

The study aimed to assess the effects of dietary extruded linseed (ELS) and Lactobacillus acidophilus (LA) on performance, carcass traits, immune organ weights, plasma lipoprotein response, and caecal bacteria populations in broiler chickens. A total of 648 one-day-old chicks were divided into six groups in a 3 × 2 factorial arrangement consisting of three ELS levels (0, 6 and 12%) without or with probiotic L. acidophilus (0 and 20 g ton⁻¹ feed). Each group had six replicates (18 birds/pen). Results showed no significant effects of ELS level nor LA supplementation on growth performance (body weight gain, feed intake, and feed conversion) of broilers during the overall period (1–42 days). The production efficiency factor increases (p = .045) with the LA addition. There were no effects of ELS level nor probiotic addition on carcase traits and immune organ weights, except for decreases in abdominal fat percentage (p = .027, respectively p = .035). The dietary ELS level significantly correlated with a decrease in the plasma total cholesterol (TC; p = .003), triglycerides, and very-low-density lipoprotein concentrations (p = .020), while the LA addition significantly correlated with a decrease (p = .015) in the plasma TC. The LA addition lowers the caecal pH (p = .007), Staphylococcus spp. and E. coli counts (p < .0001), and increase the caecal Lactobacillus spp. and lactobacilli: E. coli ratio (p < .0001). In conclusion, the use of ELS up to 12% supplemented with L. acidophilus in broiler chicks diet had a positive effect on health status, decreasing the abdominal fat deposition, plasma lipids and the caecal pathogen bacteria E. coli.

The silkworm Bombyx mori, the second most varied group of insects, is a fascinating insect that belongs to the Lepidoptera species. We aimed to deepen our knowledge about the composition and significance of amino acids (AA) from the sericulture chain to fish. AAs are the most prevalent molecules throughout the growth process of silkworms. We described AAs classification, occurrence, metabolism, and functions. Online datasets revealed that the essential AAs (EAA) level in fish meal and silkworm pupae (SWP) is comparable. SWP have a high content of methionine and lysine, which are the principal limiting AAs in fish diets, indicating that SWP have nutritional potential to be added to fish diets. Additionally, an overview of the data analyzed displays that SWP have a higher protein efficiency ratio than fish meal, the classical protein-rich source (>1.19 times), and compared to soybean meal, the second-most preferred source of protein in aquaculture (>2.08 times), indicating that SWP can be considered effective for animal feeding. In this study, we provide an overview of the current knowledge concerning AAs, paying special emphasis to EAAs and explaining, to some extent, certain mechanisms and functions of these compounds, from mulberry leaves to larvae–pupae and fish diets.