Objective: The aim of this study was to determine the effect of low-energy He-Ne laser treatment on wound healing dynamics (histological and biochemical) in diabetic rats. Background data: Low-energy laser photostimulation at certain wavelengths can enhance tissue repair by releasing growth factors from fibroblasts and can facilitate the healing process of diabetic wounds. Materials and methods: A circular 4 cm ² excisional wound was created on the dorsum of the experimentally (Alloxan)-induced diabetic rats. In the study group( N = 24) the wound was treated with He-Ne laser (632.8 nm wavelength) at a dose of 4.8 J/cm ² for 5 days a week until the wound healed completely. The control group ( N = 24) was sham-irradiated. The results were statistically analyzed by an independent t test for biochemical analysis and the nonparametric Mann-Whitney U test for histopathological parameters. Results: The analysis of the biochemical parameters and histopathological parameters of the wounds showed that the laser-treated group healed faster and better as compared to the control group ( p < 0.0001). The laser-treated group healed on average by the 18th day whereas, the control group healed on average by the 59th day. Conclusion: Laser photostimulation promotes the tissue repair process of diabetic wounds.