Chalk, an undesirable grain quality trait in rice, is primarily formed due to high temperatures during the grain-filling process. Owing to the disordered starch granule structure, air spaces and low amylose content, chalky grains are easily breakable during milling thereby lowering head rice recovery and its market price. Availability of multiple QTLs associated with grain chalkiness and associated attributes, provided us an opportunity to perform a meta-analysis and identify candidate genes and their alleles contributing to enhanced grain quality. From the 403 previously reported QTLs, 64 Meta-QTLs encompassing 5262 non-redundant genes were identified. MQTL analysis reduced the genetic and physical intervals and nearly 73% meta-QTLs were narrower than 5cM and 2Mb, revealing the hotspot genomic regions. By investigating expression profiles of 5262 genes in previously published datasets, 49 candidate genes were shortlisted on the basis of their differential regulation in at least two of the datasets. We identified non-synonymous allelic variations and haplotypes in 39 candidate genes across the 3K rice genome panel. Further, we phenotyped a subset panel of 60 rice accessions by exposing them to high temperature stress under natural field conditions over two Rabi cropping seasons. Haplo-pheno analysis uncovered haplotype combinations of two starch synthesis genes, GBSSI and SSIIa, significantly contributing towards the formation of grain chalk in rice. We, therefore, report not only markers and pre-breeding material, but also propose superior haplotype combinations which can be introduced using either marker-assisted breeding or CRISPR-Cas based prime editing to generate elite rice varieties with low grain chalkiness and high HRY traits.

In the present study, a polarization rotation switch (PRS)-based all-optical ternary inverter circuit and ternary clocked SR flip-flop (TCSR) are proposed and discussed. The present scheme is designed by the polarization rotation of light in a waveguide coupled with a micro-ring resonator (MRR). The proposed scheme uses linear polarization-encoded light. Here, the ternary (radix = 3) logical states are expressed by the different polarized light. PRS-MRR explores the polarization-encoded methodology, which depends on polarization conversion from one state to another. All-optical ultrafast switching technology is employed to design the ternary NAND gate. We develop the ternary clocked SR flip-flop by employing the NAND gate; it produces a greater number of possible outputs as compared to the binary logic clocked SR flip-flop circuit. The performance of the proposed design is measured by the Jones parameter and Stokes parameter. The results of the polarization rotation-based ternary inverter and clocked SR flip-flop are realized using a pump–probe structure in the MRR. The numerical simulation results are confirmed by the well-known Jones vector (azimuth angle and ellipticity angle) and Stokes parameter (S₁, S₂, S₃) using Ansys Lumerical Interconnect simulation software.

An analytical, two-dimensional computer model has been developed for real time prediction of 'mountain wave due to Principal mountains over Kashmir valley. Simulation of the L² profile has been made with realistic, non-zero values at higher levels and exponentially decreasing values at lower levels. Unlike Doos (1961), present solution has no restriction on the value of wave number (k). Validity of the model has been tested with the satellite observed waves in seven cases and actual aircraft report in one case.