Species distribution models (SDMs) have proven valuable in filling gaps in our knowledge of species occurrences. However, despite their broad applicability, SDMs exhibit critical shortcomings due to limitations in species occurrence data. These limitations include, in particular, issues related to sample size, positional uncertainty, and sampling bias. In addition, it is widely recognised that the quality of SDMs as well as the approaches used to mitigate the impact of the aforementioned data limitations depend on species ecology. While numerous studies have evaluated the effects of these data limitations on SDM performance, a synthesis of their results is lacking. However, without a comprehensive understanding of their individual and combined effects, our ability to predict the influence of these issues on the quality of modelled species–environment associations remains largely uncertain, limiting the value of model outputs. In this paper, we review studies that have evaluated the effects of sample size, positional uncertainty, sampling bias, and species ecology on SDMs outputs. We build upon their findings to provide recommendations for the critical assessment of species data intended for use in SDMs.

• Monitoring forest biodiversity is crucial to prevent its decline. • The Height Variation Hypothesis (HVH) is a key tool for biodiversity monitoring. • Tree Height Heterogeneity assessed by GEDI data is a good proxy of species diversity. • CHM spatial resolution, heterogeneity indices and forest density affect the HVH. • Space-borne LiDAR GEDI derived CHMs can be used to assess forest biodiversity.

Filtering approaches on Global Ecosystem Dynamics Investigation (GEDI) data differ considerably across existing studies and it is yet unclear which method is the most effective. We conducted an in‐depth analysis of GEDI's vertical accuracy in mapping terrain and canopy heights across three study sites in temperate forests and grasslands in Spain, California, and New Zealand. We started with unfiltered data (2,081,108 footprints) and describe a workflow for data filtering using Level 2A parameters and for geolocation error mitigation. We found that retaining observations with at least one detected mode eliminates noise more effectively than sensitivity. The accuracy of terrain and canopy height observations depended considerably on the number of modes, beam sensitivity, landcover, and terrain slope. In dense forests, a minimum sensitivity of 0.9 was required, while in areas with sparse vegetation, sensitivity of 0.5 sufficed. Sensitivity greater than 0.9 resulted in an overestimation of canopy height in grasslands, especially on steep slopes, where high sensitivity led to the detection of multiple modes. We suggest excluding observations with more than five modes in grasslands. We found that the most effective strategy for filtering low‐quality observations was to combine the quality flag and difference from TanDEM‐X, striking an optimal balance between eliminating poor‐quality data and preserving a maximum number of high‐quality observations. Positional shifts improved the accuracy of GEDI terrain estimates but not of vegetation height estimates. Our findings guide users to an easy way of processing of GEDI footprints, enabling the use of the most accurate data and leading to more reliable applications.

Question: Which optical traits, retrieved from biophysical models applied to Sentinel‐2 images, enable an estimation of tree species diversity based on the Spectral Variation Hypothesis?Location: Coniferous mountain forest in the eastern Italian Alps.Methods: We analyzed the PROSPECT‐5 and Invertible Forest Reflectance Model (INFORM) biophysical parameters as retrieved from canopy reflectance data of different forest plots (using Sentinel‐2 images for the years 2017, 2018 and 2019) as optical trait indicators (OTIs). We successively tested the Spectral Variation Hypothesis (SVH) for each retrieved OTI using the Rao's Q as heterogeneity index, validating them against Shannon's H values calculated as a tree species diversity index derived from in‐situ collected data.Results: We demonstrated differences among OTIs in terms of how well their variations can be linked to species diversity. In particular the variations of brown pigments (Cbrown), carotenoids (Car) and chlorophyll content (Cab) can be considered the most relevant OTIs for the application of the SVH when using the Rao's Q as a proxy for tree species diversity in our study site.Conclusions: This research underlined that the OTIs contribute differently in the SVH to estimate tree species diversity, highlighting significant positive correlations between tree species diversity and the spatial heterogeneity of the estimated pigment content (Cab, Car, Cbrown).

Forest biodiversity is a key element to support ecosystem functions. Measuring biodiversity is a necessary step to identify critical issues and to choose interventions to be applied in order to protect it. Remote sensing provides consistent quality and standardized data, which can be used to estimate different aspects of biodiversity. The Height Variation Hypothesis (HVH) represents an indirect method for estimating species diversity in forest ecosystems from the LiDAR data, and it assumes that the higher the variation in tree height (height heterogeneity, HH), calculated through the ’Canopy Height Model’ (CHM) metric, the more complex the overall structure of the forest and the higher the tree species diversity. To date, the HVH has been tested exclusively with CHM data, assessing the HH only with a single heterogeneity index (the Rao’s Q index) without making use of any moving windows (MW) approach. In this study, the HVH has been tested in an alpine coniferous forest situated in the municipality of San Genesio/Jenesien (eastern Italian Alps) at 1100 m, characterized by the presence of 11 different tree species (mainly Pinus sylvestris, Larix decidua, Picea abies followed by Betula alba and Corylus avellana). The HH has been estimated through different heterogeneity measures described in the new R rasterdiv package using, besides the CHM, also other LiDAR metrics (as the percentile or the standard deviation of the height distribution) at various spatial resolutions and MWs (ALS LiDAR data with mean point cloud density of 2.9 point/m${}^2$). For each combination of parameters, and for all the considered plots, linear regressions between the Shannon’s H′ (used as tree species diversity index based on field data) and the HH have been derived. The results showed that the Rao’s Q index (singularly and through a multidimensional approach) performed generally better than the other heterogeneity indices in the assessment of the HH. The CHM and the LiDAR metrics related to the upper quantile point cloud distribution at fine resolution (2.5 m, 5 m) have shown the most important results for the assessment of the HH. The size of the used MW did not influence the general outcomes but instead, it increased when compared to the results found in the literature, where the HVH was tested without MW approach. The outcomes of this study underline that the HVH, calculated with certain heterogeneity indices and LiDAR data, can be considered a useful tool for assessing tree species diversity in considered forest ecosystems. The general results highlight the strength and importance of LiDAR data in assessing the height heterogeneity and the related biodiversity in forest ecosystems.